In vitro induction and regeneration of tetraploids and mixoploids of two cassava cultivars

Abstract We report the in vitro induction of polyploids from two cassava cultivars by colchicine treatment. Shoot nodal segments, collected from in vitro cultivated plants of Porquinho and Vassourinha cultivars, were used as explants. Explants were treated in liquid media without and with colchicine (0.05, 0.10 or 0.15%), at 90 rpm, in the dark, for 48 or 96 h. Normal-like and putative-polyploid phenotypes were recovered from in vitro culture, for both cultivars. All plants from the putative-polyploid group were confirmed as tetraploids by flow cytometry and root tip chromosome counting, where...