Identification of Antifungal Compounds from the Root Bark of Cordia anisophylla J.S. Mill.

The dichloromethane extract of the root bark of the Panamanian plant Cordia anisophylla J.S. Mill. (Boraginaceae) presented antifungal activity against a susceptible strain of Candida albicans in a bioautography primary screening. The susceptible strain was used to detect minor active compounds that would not have been detected using a classical approach. In order to identify the antimicrobial compounds, the active extract was fractionated by semi-preparative high-performance liquid chromatography and the fractions were submitted to the antifungal bioassay. This procedure enabled a precise loc...