Coupling DLLME-CE for the Stereoselective Analysis of Venlafaxine and Its Main Metabolites after Biotransformation by Fungi

Fungal biotransformations have become very important in the study of chiral drugs because the reactions performed by these microorganisms may be enantioselective. However, analyses of analytes present in liquid culture medium have proved to be very difficult due to the complexity of this matrix. The aim of this work was to couple dispersive liquid-liquid microextraction (DLLME) with capillary electrophoresis to evaluate the biotransformation of the antidepressant drug venlafaxine (Vx) into its chiral metabolites, N-desmethylvenlafaxine (NDV) and O-desmethylvenlafaxine (ODV) by fungi. The chira...