Characterization of Phytophthora nicotianae isolates from tobacco plants (Nicotiana tabacum) in Colombia

ABSTRACT The black shank disease caused by Phytophthora nicotianae causes losses in tobacco crops up 100%. In Colombia, P. nicotianae populations are poorly known causing wrong diagnostics and erratic management. Amplification of the Ypt1 gene and morphological characteristics of colonies, sporangia, chlamydospores and hyphae were used to identify P. nicotianae isolates. Races were identified according to the reaction induced by each isolate on the differential tobacco varieties Hicks, L8, KY 14 x L8 and NC 1071. As results, 71 isolates of P. nicotianae were identified and classified by races....

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