20/07/2020

CHARACTERIZATION OF INTERACTION BETWEEN BUDDLEOSIDE AND BOVINE SERUM ALBUMIN

The primary objective of this study was to investigate the binding of buddleoside to bovine serum albumin (BSA) by different analytical techniques. The analytical principles of affinity capillary electrophoresis (ACE) and fluorescence spectroscopy used in the experiments were different. Mobility (M) that is independent of viscosity of the operating buffer and voltage was chosen to eliminate the effects of electroosmotic flow (EOF) in ACE and was applied to estimate the binding constant Ka. The quenching constant Ksv, binding site number n and binding constant Kb were provided by the fluorescen...

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