Biosynthesis of selenium nanoparticles using Klebsiella pneumoniae and their recovery by a simple sterilization process

The use of biologically derived metal nanoparticles for various proposes is going to be an issue of considerable importance; thus, appropriate methods should be developed and tested for the biological synthesis and recovery of these nanoparticles from bacterial cells. In this research study, a strain of Klebsiella pneumoniae was tested for its ability to synthesize elemental selenium nanoparticles from selenium chloride. A broth of Klebsiella pneumoniae culture containing selenium nanoparticles was subjected to sterilization at 121ºC and 17 psi for 20 minutes. Released selenium nanoparticles ...