AVALIAÇÃO DE ADITIVOS DE FASE MÓVEL E TEMPERATURA NA VIDA ÚTIL DE COLUNAS PARA CROMATOGRAFIA LÍQUIDA DE ALTA EFICIÊNCIA

Buffered solutions, additives, and column temperature are widely used in high-performance liquid chromatography to promote adequate retention of compounds and to improve the chromatographic separation. However, these factors can dramatically reduce the column lifetime. In this work, the effect of column temperature, pH and the salt type and concentration in buffered mobile phases were evaluated on the stability of a typical commercial C18 stationary phase. A method for rapid evaluation of stationary phase degradation caused by these variables was employed to avoid the generation of large volum...