APPLICATION OF RESPONSE SURFACE METHODOLOGY FOR LIPASE CATALYZED SYNTHESIS OF 2-ETHYLHEXYL PALMITATE IN A SOLVENT FREE SYSTEM USING ULTRASOUND

Abstract This study aims at clean production of 2-ethylhexyl palmitate catalyzed by lipase Fermase CALB 10000 in a solvent-free system using ultrasound technology. The central composite design (CCD) of response surface methodology with 4 factors at 3 levels consisting of acid to alcohol molar ratio (1:3-1:7), enzyme loading (3 %-7% (w/w)), ultrasound power (60 - 100 W) and duty cycle (30%-70 %) was used for optimization of the reaction. All the reactions were carried out at a fixed temperature of 60°C, 200 rpm and reaction time (120 min). The statistical software, Design-Expert 9.0, was used ...