Aphelenchoides besseyi Christie (Nematoda: Aphelenchoididae), agente causal del amachamiento del frijol común

Identification of the causal agent of the disease "amachamiento" of common bean was carried out using four tests: analysis of double strand RNA (dsRNA) of viral origin, DAS-ELISA technique, applying Koch's postulates and the pathogenicity test. The analysis to detect dsRNA brought about two bands of approximately 1100 and 3200 bp, a similar pattern of bands for a member of genus Bromovirus. However, those bands were present only in 55.5% of the diseased plant samples analyzed; consequently, it was not possible to establish an association of a virus with the disease. The DAS-ELISA test did not ...