Anatomical and histochemical studies of the somatic embryogenesis of Syagrus oleracea from immature inflorescences

Abstract Anatomical and histochemical studies were carried out during the somatic embryogenesis of Syagrus oleracea (Mart.) Becc. from immature inflorescences. Immature rachillas were inoculated on Murashige and Skoog medium with 4.52 μM 4-amino-3,5,6-trichloropicolinic acid (Picloram) and 2,4-diclorophenoxyacetic acid. Auxin concentrations were gradually reduced for the formation of somatic embryos, while for plantlet regeneration, embryos were inoculated on a medium without growth regulator. After 270 days, two types of embryogenic calluses were observed in explants under the effect of Picl...