An eco-friendly method for extraction and quantification of flavonoids in Dysphania ambrosioides

ABSTRACT Dysphania ambrosioides (L.) Mosyakin and Clemants (Syn: Chenopodium ambrosioides L.), Amaranthaceae, is a plant with antibacterial, antifungal, antioxidant, antiparasitic and antitumor properties that is commonly used in Brazilian folk medicine. In this work we performed the optimization of ultrasound-assisted extraction of flavonoids in the aerial parts of D. ambrosioides. The flavonoid concentrations, as rutin equivalents, were quantified with the aid of a validated spectrophotometric method. The Box–Behnken (33) design with response surface methodology, for the independent variab...