Technical Reproducibility
Mostrando 25-36 de 39 artigos, teses e dissertações.
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25. Attachment of Mycoplasma pneumoniae to hamster tracheal organ cultures, tracheal outgrowth monolayers, human erythrocytes, and WiDr human tissue culture cells.
Virulent strains of Mycoplasma pneumoniae, PI-1428 and M129, were radiolabeled wtih [3H]palmitic acid or [3H]thymidine and examined for attachment to hamster tracheal organ cultures, tracheal outgrowth monolayers, human O-positive erythrocytes, and human WiDr carcinoma cell cultures. Although attachment to each cell substrate was readily detected, the WiDr c
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26. Multicenter evaluation of arbitrarily primed PCR for typing of Staphylococcus aureus strains.
Fifty-nine isolates of Staphylococcus aureus and a single strain of Staphylococcus intermedius were typed by arbitrarily primed PCR (AP-PCR). To study reproducibility and discriminatory abilities, AP-PCR was carried out in seven laboratories with a standardized amplification protocol, template DNA isolated in a single institution, and a common set of three p
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27. Comparison of two methods for bacteriocin typing of Serratia marcescens.
Two methods of bacteriocin susceptibility typing for Serratia marcescens were compared. A total of 80 epidemiologically unrelated isolates from patients in a single hospital were typed by the cross-streaking method and the mitomycin C-induced (spotting) method. The cross-streaking method was found to be more discriminatory than the spotting method. Using the
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28. HLA-B27 associated cross-reactive marker on the cells of New Zealand patients with ankylosing spondylitis.
We have previously shown that antibodies raised in rabbits to certain enteric bacteria will specifically lyse, in a 51Cr release assay, the peripheral blood lymphocytes (PBL) of 80% of HLA-B27 positive patients with ankylosing spondylitis (B27+ AS+) but not the PBL of HLA-B27 positive normal controls (B27+ AS-). Other laboratories have been unable to reprodu
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29. Hepatitis C Virus (HCV) Core Antigen Assay To Detect Ongoing HCV Infection in Thai Injection Drug Users
We evaluated a quantitative enzyme immunoassay (trak-C) for hepatitis C virus core antigen (HCV core Ag) by testing serum specimens from 820 injection drug users in Thailand with anti-HCV antibodies. The HCV genotypes in this population include genotypes 3 and 6, which have not been extensively tested with this assay. Among these specimens, 629 (76.7%) yield
American Society for Microbiology.
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30. Assessment of Resolution and Intercenter Reproducibility of Results of Genotyping Staphylococcus aureus by Pulsed-Field Gel Electrophoresis of SmaI Macrorestriction Fragments: a Multicenter Study
Twenty well-characterized isolates of methicillin-resistant Staphylococcus aureus were used to study the optimal resolution and interlaboratory reproducibility of pulsed-field gel electrophoresis (PFGE) of DNA macrorestriction fragments. Five identical isolates (one PFGE type), 5 isolates that produced related PFGE subtypes, and 10 isolates with unique PFGE
American Society for Microbiology.
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31. Molecular Genotyping of Hydatidiform Moles : Analytic Validation of a Multiplex Short Tandem Repeat Assay
Distinction of hydatidiform moles from non-molar (NM) specimens, as well as their subclassification as complete (CHM) versus partial hydatidiform moles (PHM), is important for clinical management and accurate risk assessment for persistent gestational trophoblastic disease. Because diagnosis of hydatidiform moles based solely on morphology suffers from poor
American Society for Investigative Pathology.
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32. Development of an internal quality assessment scheme in a clinical bacteriology laboratory.
AIM--To develop an internal quality assessment (IQA) scheme in a clinical bacteriology laboratory. METHODS--Over 24 months, 1230 diagnostic specimens, representing 0.42% of laboratory workload, were anonymised and resubmitted for analysis. Six hundred and twenty one (48.7%) of these gave positive culture results; 44 fecal and upper respiratory specimens were
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33. Failure of ST segment elevation to predict severity of acute myocardial infarction.
Praecordial ST segment elevation was measured at 35 electrode positions in each of 40 patients admitted to a coronary care unit after acute transmural anterior myocardial infarction. Serial praecordial electrocardiographic maps were recorded to determine (a) the time course as well as reproducibility of measurements of ST segment alterations, and (b) the deg
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34. Comprehensive comparison of six microarray technologies
Microarray technology is extensively used in biological research. The applied technologies vary greatly between laboratories, and outstanding questions remain regarding the degree of correlation among approaches. Recently, there has been a drive toward ensuring high-quality microarray data by the implementation of MIAME (Minimal Information About a Microarra
Oxford University Press.
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35. Profiling of Arabidopsis Secondary Metabolites by Capillary Liquid Chromatography Coupled to Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry1
Large-scale metabolic profiling is expected to develop into an integral part of functional genomics and systems biology. The metabolome of a cell or an organism is chemically highly complex. Therefore, comprehensive biochemical phenotyping requires a multitude of analytical techniques. Here, we describe a profiling approach that combines separation by capill
The American Society for Plant Biologists.
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36. Real-Time PCR-Based System for Simultaneous Quantification of Human Papillomavirus Types Associated with High Risk of Cervical Cancer
We have previously shown that women with a high titer of human papillomavirus type 16 (HPV16) in cervical epithelial cells have an increased risk of developing cervical carcinoma in situ. In order to study the relationship between viral DNA amount and risk of cervical carcinoma for the HPV types most commonly found in cervical tumors, we developed a real-tim
American Society for Microbiology.