Target Vectors
Mostrando 13-24 de 432 artigos, teses e dissertações.
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13. 3D image registration of the human brain / Registro de imagens 3D do cerebro humano
Image Registration is the process that aligns two or more images in a common reference system of spacial coordinates [31]. It is an important problem with several applications in Medical Imaging, enabling, for instance, the analysis of changes in anatomy along time by the registration of images from the same modality, and the study of combined anatomic and p
Publicado em: 2009
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14. Padronização da expressão heterologa e de modelo de ensaio de atividade para a proteina quinase humana S6K / Standardization of the heterologous expression and of a model assay of activity for the human protein kinase S6K
The 70kDa ribosomal S6 protein kinase 1 (S6K1) is a phosphoprotein involved in the regulation of genes related to translational control in mammals. S6K1 shows distinct nuclear (a1) and cytoplasmic (a2) forms. Phosphorylation of the S6K1 best characterized target, the protein of the small ribosomal subunit (RPS6), has been generally associated to the selectiv
Publicado em: 2009
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15. Development and evaluation of immunization tools based on the adenovirus fiber knob modified with the C4 domain of HIV gp120 glycoprotein. / Desenvolvimento e avaliação de ferramentas de imunização baseadas na região globular da fibra adenoviral modificada com o domínio C4 da glicoproteína gp120 do HIV.
HIV glycoprotein gp120 has conserved domains, one of them being the C4 domain. This region is involved in the recognition of the CD4 marker in target cells and antibodies that recognize this domain can block HIV infection. Previously, the C4 domain was introduced in the adenovirus fiber knob. As the adenovirus fiber stimulates de immune system, we decided to
Publicado em: 2008
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16. Construction of auxotrophic marker in Mycobacterium bovis BCG, knockout strain for the DPPD and proteomic study of tuberculin / Construção de marcador auxotrófico em Mycobacterium bovis BCG, de uma cepa knockout para DPPD e estudo proteômico da tuberculina
Mycobacterium bovis BCG has the potential to be an effective live vector for multivalent vaccines. However, there are two problems regarding the utilization of recombinant BCG as vaccine. The first one is that most mycobacterial cloning vectors rely on antibiotic resistance gene as selectable marker, which is used for genetic transformation. The second one i
Publicado em: 2008
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17. A construção de um Indice de Sustentabilidade Ambiental Agricola (ISA) : uma proposta metodologica / The construction of the agricultural environmental sustainability index (ISAGRI) : a methodological proposal
This study presents the methodology of construction of the Agricultural Environmental Sustainability Index (ISAGRI). This Index was evaluated in the watersheds of the rivers Oriçanga and Araras, both belonging to the Mogi Guaçu river basin. This is a representative area of different situations found in the State of São Paulo. The region presents practical
Publicado em: 2007
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18. City Marketing, Empresarialismo TurÃstico e Desenvolvimento Local SustentÃvel no MunicÃpio de TamandarÃ-PE
The main purpose of this dissertation work is to build reflections on the importance of the role of city marketing and tourist enterprising in the promotion of local sustainable development, aiming at Tamandarà city, in the state of Pernambuco, as a possible target to public power for implementing alternatives to dynamise tourist activity. Based on a readin
Publicado em: 2007
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19. Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element
The technique to generate transgenic mosquitoes requires adaptation for each target species because of aspects related to species biology, sensitivity to manipulation and rearing conditions. Here we tested different parameters on the microinjection procedure in order to obtain a transgenic Neotropical mosquito species. By using a transposon-based strategy we
Memórias do Instituto Oswaldo Cruz. Publicado em: 2006-11
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20. Production and characterization of gum operon mutants of Xylella fastidiosa cvc strain. / Produção e caracterização de mutantes do operon gum de Xylella fastidiosa.
Xylella fastidiosa is a fastidious, xylem restricted, gram.negative bacteria, that causes several economically important diseases as Pierce s disease of grapevine in USA and the Citrus Variegated Chlorosis (CVC) in Brasil. CVC affects severely the São Paulo State citriculture jeopardizing thousands of jobs and millions of dollars of incomes. The genome sequ
Publicado em: 2003
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21. Characterization of constitutive and putative differentially expressed mRNAs by means of expressed sequence tags, differential display reverse transcriptase-PCR and randomly amplified polymorphic DNA-PCR from the sand fly vector Lutzomyia longipalpis
Molecular studies of insect disease vectors are of paramount importance for understanding parasite-vector relationship. Advances in this area have led to important findings regarding changes in vectors' physiology upon blood feeding and parasite infection. Mechanisms for interfering with the vectorial capacity of insects responsible for the transmission of d
Memórias do Instituto Oswaldo Cruz. Publicado em: 2001-01
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22. Cellular immune response to adenoviral vector infected cells does not require de novo viral gene expression: Implications for gene therapy
Replication-defective adenoviral (RDAd) vectors can be generated at high titers and infect both dividing and nondividing cells. Long term expression in the transduced tissue, however, has been a problem because of the cellular immune responses against the infected cells. We demonstrate that mice injected with RDAd vectors containing mouse leptin gene reduce
The National Academy of Sciences.
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23. Reexamination of gene targeting frequency as a function of the extent of homology between the targeting vector and the target locus.
Mutations were targeted to the Hprt locus of mouse embryo-derived stem cells by using 22 different sequence replacement and sequence insertion vectors. The targeting frequency was examined at two sites within the Hprt locus as a function of the extent of homology between the targeting vector and the target locus. The targeting frequency was also compared by
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24. Altering the insertional specificity of a Drosophila transposable element.
Vectors derived from the Drosophila P element transposon are widely used to make transgenic Drosophila. Insertion of most P-element-derived vectors is nonrandom, but they exhibit a broad specificity of target sites. During experiments to identify cis-acting regulatory elements of the Drosophila segmentation gene engrailed, we identified a fragment of engrail