Tail Pcr
Mostrando 1-12 de 90 artigos, teses e dissertações.
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1. Exosomes secreted from miRNA-29b-modified mesenchymal stem cells repaired spinal cord injury in rats
Exosomes, a kind of extracellular vesicle, are promising therapeutic agents for spinal cord injury (SCI). This article aimed to investigate effects of exosomes secreted from miRNA-29b-modified bone marrow mesenchymal stem cells (BMSCs) on SCI. Exosomes were extracted from BMSCs transfected with miRNA-29b or negative control (miR NC). SCI rats were injected i
Braz J Med Biol Res. Publicado em: 05/12/2019
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2. Assesment of the Genotoxic Effect of the Diazinon on Root Cells of Allium cepa (L.)
ABSTRACT Organophosphorous pesticides (OPs) posses a great potential of acute toxicity for exposed animals and men. To evaluate the toxic potential of the organophosphate diazinon on root meristematic cells of Allium cepa L., was created two groups: In group 1 (control group), was not given any chemical. In group 2 (diazinon-treatment group), different doses
Braz. arch. biol. technol.. Publicado em: 06/08/2018
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3. Total flavonoids from Ampelopsis megalophylla suppress proliferation of vascular smooth muscle cells in vivo and in vitro
ABSTRACT Various benefits of flavonoids for ameliorating cardiovascular diseases have been demonstrated. However, the lowering effects on blood pressure caused by antiproliferative potentials of flavonoids in vascular smooth muscle cells are rare. In this study, the antihypertensive effects of total flavonoids from Ampelopsis megalophylla were investigated.
Braz. J. Pharm. Sci.. Publicado em: 01/02/2018
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4. Molecular analysis of CIB4 gene and protein in Kermani sheep
The human calcium- and integrin-binding protein (CIB) family is composed of CIB1, CIB2, CIB3, and CIB4 proteins and the CIB4 gene affects fertility. Kermani sheep is one of the most important breeds of Iranian sheep breeds. The aim of this study was to analyze for the first time molecular characteristics of the CIB4 gene and protein in Kermani sheep. Differe
Braz J Med Biol Res. Publicado em: 12/09/2017
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5. Characterization of cry2-type genes of Bacillus thuringiensis strains from soil-isolated of Sichuan basin, China
Sichuan basin, situated in the west of China, is the fourth biggest basin in China. In order to describe a systematic study of the cry2-type genes resources from Bacillus thuringiensis strains of Sichuan basin, a total of 791 Bacillus thuringiensis strains have been screened from 2650 soil samples in different ecological regions. The method of PCR-restrictio
Brazilian Journal of Microbiology. Publicado em: 2011-03
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6. Alternative PCR protocol using a single primer set for assessing DNA quality in several tissues from a large variety of mammalian species living in areas endemic for leishmaniasis
The aim of this work was to establish a modified pre-diagnostic polymerase chain reaction (PCR) protocol using a single primer set that enables successful amplification of a highly conserved mammalian sequence in order to determine overall sample DNA quality for multiple mammalian species that inhabit areas endemic for leishmaniasis. The gene encoding interp
Memórias do Instituto Oswaldo Cruz. Publicado em: 2010-11
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7. Non genomic actions of triiodothyronine (T3) on the expression polyadenylation and distribution of TSH granules in thyrotrophs of hypothyroid rats / Ações não genômicas da triiodotironina (T3) sobre a expressão, poliadenilação e distribuição dos grânulos de TSH nos tireotrofos de ratos hipotireiodeos
The thyroid-stimulating hormone (TSH) is the main regulator of the synthesis and secretion of thyroid hormones (TH), which exert a negative feedback mechanism in the pituitary by reducing the synthesis of β and α (CGA - Glycoprotein hormones alpha chain) chains through mechanisms that involve changes in the tra
Publicado em: 2010
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8. Clonagem e estudo funcional do promotor do gene DREB da mamoneira (Ricinus communis L.)
Um dos principais estresses que restringem a capacidade e a eficiência da planta realizar os processos bioquímicos importantes é a falta de água. As conseqüências para o vegetal vão desde alterações no volume celular à desnaturação de importantes proteínas, e dependendo da intensidade do estresse, poderá ocasionar a morte da planta. Com isso, a
Publicado em: 2008
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9. Connexins in the early development of the African clawed frog Xenopus laevis (Amphibia): The role of the connexin43 carboxyl terminal tail in the establishment of the dorso-ventral axis
Connexins are a family of related proteins identified in vertebrate forming gap junctions, which mediate cell-to-cell communication in early embryos, with an important role in establishing embryonic asymmetry and ‘communication compartments’. By in situ hybridization, immunocytochemistry, reverse transcriptase PCR (RT-PCR) and western blotting we show th
Genetics and Molecular Biology. Publicado em: 2007-03
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10. Application of a double-enrichment procedure for microsatellite isolation and the use of tailed primers for high throughput genotyping
The number of microsatellite loci and their allelic diversity contribute to increase accuracy and informativity of genetic estimates, however, the isolation of microsatellite loci is not only laborious but also quite expensive. We used (GATA)n and (GACA)n tetranucleotide probes and single- and double-enrichment hybridization to construct and screen a genomic
Genetics and Molecular Biology. Publicado em: 2007-03
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11. Estudo de proteases degradadoras de cutícula produzidas por Beauveria bassiana
The entomopathogenic fungus Beauveria bassiana is considered a promising agent of microbial control of insects considered pests of agriculture, such as, the grasshopper Rhammatocerus schistocercoides. This grasshopper attacks economically important plantations in the center-west Brazil. B. bassiana is capable to penetrate through cuticle of the host by apres
Publicado em: 2007
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12. Toxinas Cry : perspectivas para a obtenção de algodão transgênico brasileiro
Toxinas Cry provenientes de Bacillus thuringiensis, também denominadas de δ- endotoxinas, possuem ampla atividade inseticida e são específicas para insetos de diferentes ordens, não sendo nocivas a mamíferos, aves, anfíbios ou répteis. Devido a esta característica, seus genes vêm sendo utilizados no desenvolvimento de plantas transgênicas, visando
Publicado em: 2007