T4 Lysozyme
Mostrando 1-12 de 132 artigos, teses e dissertações.
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1. Estudos da dinâmica estrutural da proteína ligante de cálcio S100A12 humana e da lisozima T4 / Structural dynamics studies of human calcium binding protein S100A12 and T4 lysozyme
The work presented here was conceived with two main objectives. The first one, more general, involved the implementation of a new methodology for the study of conformational changes in proteins, i.e., its structural dynamics. The technique of Site-directed Spin Labeling combined with Electronic Paramagnetic Resonance (SDSL-EPR) are the pillars of this new me
Publicado em: 2011
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2. Avaliação do papel da galectina-3 sobre funções de neutrófilos durante a infecção por cepa virulenta RH de Toxoplasma gondii em camundongos C57BL/6
Galectins are beta-galactoside-binding animal lectins involved in several biological processes, and Gal-3 has been related to modulation of immune reactions and inflammatory responses. The present study aimed to evaluate the influence of Gal-3 in neutrophil viability, early apoptosis and cell death in parallel with its biological functions after in vivo and
Publicado em: 2009
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3. Stabilization of serum antibody responses triggered by initial mucosal contact with the antigen independently of oral tolerance induction
Initial contacts with a T-dependent antigen by mucosal routes may result in oral tolerance, defined as the inhibition of specific antibody formation after subsequent parenteral immunizations with the same antigen. We describe here an additional and permanent consequence of these initial contacts, namely, the blockade of secondary-type responsiveness to subse
Brazilian Journal of Medical and Biological Research. Publicado em: 2001-02
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4. BACTERIOPHAGE T4 DNA-DEPENDENT in vitro SYNTHESIS OF LYSOZYME*
A cell-free system derived from uninfected Escherichia coli previously was shown to synthesize β-glucosyl transferase in response to T4 DNA. This same in vitro system, when incubated at slightly higher magnesium concentrations, also synthesized enzymatically active lysozyme. The lysozyme activity that appeared was judged to be T4-specific since antibodies p
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5. Increased Killing of Bacillus subtilis on the Hair Roots of Transgenic T4 Lysozyme-Producing Potatoes
Transgenic potato plants expressing the phage T4 lysozyme gene which are resistant to the plant-pathogenic enterobacterium Erwinia carotovora subsp. carotovora have been constructed. The agricultural growth of these potatoes might have harmful effects on soil microbiota as a result of T4 lysozyme release into the rhizosphere. To assess the bactericidal effec
American Society for Microbiology.
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6. Isolation and characterization of the bacteriophage T4 tail-associated lysozyme.
Direct evidence has been obtained that the tail-associated lysozyme of bacteriophage T4 (tail-lysozyme) is gp5, which is a protein component of the hub of the baseplate. Tails were treated with 3 M guanidine hydrochloride containing 1% Triton X-100, and the tail-lysozyme was separated from other tail components by preparative isoelectric focusing electrophor
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7. Bacteriophage Lysozyme Synthesis in Escherichia coli K-12(λ) Infected with rII Mutant of Bacteriophage T4
When Escherichia coli K-12(λ) was infected with a T4rII bacteriophage, synthesis of lysozyme appeared at the normal time but stopped 15 min after infection. The lysozyme produced was 1% of the normal level.
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8. The Chain Growth Rate of T4 Lysozyme in vitro*
The average interval between attachment of a 30S ribosomal subunit to bacteriophage T4 lysozyme mRNA and the completion of synthesis of lysozyme protein has been measured in vitro. The measured completion times yield rates of polypeptide chain propagation of 1.6 and 3.0 amino acids per second at 25°C and 31°C, respectively.
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9. Effects of T4 Lysozyme Release from Transgenic Potato Roots on Bacterial Rhizosphere Communities Are Negligible Relative to Natural Factors
Rhizosphere bacterial communities of two transgenic potato lines which produce T4 lysozyme for protection against bacterial infections were analyzed in comparison to communities of wild-type plants and transgenic controls not harboring the lysozyme gene. Rhizosphere samples were taken from young, flowering, and senescent plants at two field sites in three co
American Society for Microbiology.
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10. Mutations in an upstream regulatory sequence that increase expression of the bacteriophage T4 lysozyme gene.
A P22 hybrid phage bearing the bacteriophage T4 lysozyme gene (e), as well as T4 sequences upstream from the lysozyme gene, was constructed. Amber mutations were introduced into gene e in the hybrid phage, and the resulting mutant phages were tested for the ability to form plaques on amber suppressor strains. Revertant phages that were able to form plaques o
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11. Inactivation of Gram-Negative Bacteria by Lysozyme, Denatured Lysozyme, and Lysozyme-Derived Peptides under High Hydrostatic Pressure
We have studied the inactivation of six gram-negative bacteria (Escherichia coli, Pseudomonas fluorescens, Salmonella enterica serovar Typhimurium, Salmonella enteritidis, Shigella sonnei, and Shigella flexneri) by high hydrostatic pressure treatment in the presence of hen egg-white lysozyme, partially or completely denatured lysozyme, or a synthetic cationi
American Society for Microbiology.
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12. Structural basis of the conversion of T4 lysozyme into a transglycosidase by reengineering the active site
In contrast to hen egg-white lysozyme, which retains the β-configuration of the substrate in the product, T4 lysozyme (T4L) is an inverting glycosidase. The substitution Thr-26 → His, however, converts T4L from an inverting to a retaining enzyme. It is shown here that the Thr-26 → His mutant is also a transglycosidase. Indeed, the transglycosylation rea
The National Academy of Sciences.