Suppressive Subtractive Hybridization
Mostrando 1-12 de 14 artigos, teses e dissertações.
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1. Expressão diferencial de genes induzidos por antracnose em feijoeiro em resposta à indução da resistência por silício / Differential expression of genes activated by anthracnose in response to silicon induced resistance
O feijão é importante fonte carboidratos, vitaminas, minerais e fibras. No Brasil, a produtividade desta leguminosa é baixa e um dos fatores é a ocorrência de doenças como a antracnose causada pelo Colletothrichum lindemuthianum, que gera perdas de até 100% da produção. Plantas possuem diversos mecanismos de defesa contra patógenos e relatos aponta
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 08/08/2012
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2. Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit
Soybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, w
Genetics and Molecular Biology. Publicado em: 2012
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3. Identificação de genes expressos durante a fase pré-simbiótica da associação ectomicorrízica entre Hydnangium sp. e Eucalyptus grandis e transformação de fungos ectomicorrízicos / Identification of genes expressed during the pre-symbiotic interaction in the Hydnangium sp.-Eucalyptus grandis ectomycorrhizal association and transformation of ectomycorrhizal fungi
Gene expression in the ectomycorrhizal fungus Hydnangium sp. during the pre-symbiotic interaction was evaluated using an in vitro mycorrhization technique aiming at constructing a suppressive subtractive cDNA library. The fungus was cultivated in the presence of Eucalyptus grandis roots, with no direct physical contact between both organisms. Genes that code
Publicado em: 2008
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4. Identification and characterization of genes differentially expressed during arbuscular mycorrhiza development in sugarcane / Identificação e caracterização de genes com expressão diferencial durante o desenvolvimento de micorrizas arbusculares em cana-de-açúcar
Arbuscular mycorrhizae (AM) are mutualistic symbiotic associations between fungi of the order Glomales and most of the terrestrial plants. The molecular mechanisms regulating AM development and efficiency are not well understood so that agricultural large scale application is so difficult. Although so many approaches are being used to the identification of d
Publicado em: 2008
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5. Identification of Bacillus anthracis specific chromosomal sequences by suppressive subtractive hybridization
BioMed Central.
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6. Subtractive Hybridization Reveals a Type I Polyketide Synthase Locus Specific to Mycobacterium ulcerans
Mycobacterium ulcerans causes Buruli ulcer, the third most prevalent mycobacterial infection of immunocompetent humans after tuberculosis and leprosy. Recent work has shown that the production by M. ulcerans of mycolactone, a novel polyketide, may partly explain the pathogenesis of Buruli ulcer. To search for the genetic basis of virulence in M. ulcerans, we
American Society for Microbiology.
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7. Suppressive Subtractive Hybridization Detects Extensive Genomic Diversity in Thermotoga maritima
Comparisons between genomes of closely related bacteria often show large variations in gene content, even between strains of the same species. Such studies have focused mainly on pathogens; here, we examined Thermotoga maritima, a free-living hyperthermophilic bacterium, by using suppressive subtractive hybridization. The genome sequence of T. maritima MSB8
American Society for Microbiology.
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8. The DNA/RNA-binding protein MSY2 marks specific transcripts for cytoplasmic storage in mouse male germ cells
During spermatogenesis, male germ cells temporally synthesize many proteins as they differentiate through meiosis and become spermatozoa. The germ cell Y-box protein, MSY2, constituting ≈0.7% of total protein in male germ cells, binds to a consensus promoter element, and shows a general lack of RNA-binding specificity. Combining immunoprecipitation and sup
National Academy of Sciences.
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9. Molecular Characterization of Brucella abortus Chromosome II Recombination
Large-scale genomic rearrangements including inversions, deletions, and duplications are significant in bacterial evolution. The recently completed Brucella melitensis 16M and Brucella suis 1330 genomes have facilitated the investigation of such events in the Brucella spp. Suppressive subtractive hybridization (SSH) was employed in identifying genomic differ
American Society for Microbiology.
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10. Pseudomonas fluorescens and Glomus mosseae Trigger DMI3-Dependent Activation of Genes Related to a Signal Transduction Pathway in Roots of Medicago truncatula1
Plant genes induced during early root colonization of Medicago truncatula Gaertn. J5 by a growth-promoting strain of Pseudomonas fluorescens (C7R12) have been identified by suppressive subtractive hybridization. Ten M. truncatula genes, coding proteins associated with a putative signal transduction pathway, showed an early and transient activation during ini
American Society of Plant Biologists.
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11. Survey of Transcript Expression in Rainbow Trout Leukocytes Reveals a Major Contribution of Interferon-Responsive Genes in the Early Response to a Rhabdovirus Infection
Virus infections induce changes in the expression of host cell genes. A global knowledge of these modifications should help to better understand the virus/host cell interactions. To obtain a more comprehensive view of the rainbow trout response to a viral infection, we used the subtractive suppressive hybridization methodology in the viral hemorrhagic septic
American Society for Microbiology.
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12. Embryonic diapause highlighted by differential expression of mRNAs for ecdysteroidogenesis, transcription and lipid sparing in the cricket Allonemobius socius
Embryos of the ground cricket, Allonemobius socius, enter diapause 4–5 days post-oviposition and overwinter in this dormant state that is characterized by developmental arrest. Suppressive subtractive hybridization and quantitative real-time PCR reveal eight candidate genes in pre-diapause embryos that show promise as regulators of diapause entry, when
Company of Biologists.