Suppression Subtractive Hybridization
Mostrando 1-12 de 57 artigos, teses e dissertações.
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1. Down-regulation of single-stranded DNA-binding protein 1 expression induced by HCMV infection promotes lipid accumulation in cells
The objective of this study was to observe the infection of human cytomegalovirus (HCMV) to human umbilical vein endothelial cells, and its effect on the expression of single-stranded DNA-binding protein (SSBP1) and on lipid metabolism in endothelial cells. We screened the differential expression of mRNAs after HCMV infection by suppression subtractive hybri
Braz J Med Biol Res. Publicado em: 12/09/2017
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2. Expression of stress-related genes in zebrawood (Astronium fraxinifolium, Anacardiaceae) seedlings following germination in microgravity
Seeds of a tropical tree species from Brazil, Astronium fraxinifolium, or zebrawood, were germinated, for the first time in microgravity, aboard the International Space Station for nine days. Following three days of subsequent growth under normal terrestrial gravitational conditions, greater root length and numbers of secondary roots was observed in the micr
Genet. Mol. Biol.. Publicado em: 2014
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3. Differential gene expression, induced by salicylic acid and Fusarium oxysporum f. sp. lycopersici infection, in tomato.
The objective of this work was to determine the transcript profile of tomato plants (Lycopersicon esculentum Mill.), during Fusarium oxysporum f. sp. lycopersici infection and after foliar application of salicylic acid. The suppression subtractive hybridization (SSH) technique was used to generate a cDNA library enriched for transcripts differentially expr
Pesquisa Agropecuária Brasileira. Publicado em: 2011
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4. Isolation and characterization of genes functionally involved in ovarian development of the giant tiger shrimp Penaeus monodon by suppression subtractive hybridization (SSH)
Suppression subtractive hybridization (SSH) libraries between cDNA in stages I (previtellogenic) and III (cortical rod) ovaries of the giant tiger shrimp (Penaeus monodon) were established. In all, 452 ESTs were unidirectionally sequenced. Sequence assembly generated 28 contigs and 201 singletons, 109 of which (48.0%) corresponding to known sequences previou
Genetics and Molecular Biology. Publicado em: 2010
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5. Differentially expressed genes in a flock of Chinese local-breed chickens infected with a subgroup J avian leukosis virus using suppression subtractive hybridization
Avian leukosis virus subgroup J (ALV-J) is a new type of virus that mainly induces myeloid leukosis (ML) in chickens. To further elucidate the pathogenesis of ALV-J infection and tumor development, expression profiles from the bone marrow tissue of 15 infected and 18 non-infected birds from a local-breed poultry-farm under naturally infected conditions, were
Genetics and Molecular Biology. Publicado em: 11/12/2009
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6. Respostas à deficiência de fósforo e suas relações com a tolerância ao alumínio em trigo / Phosphorus deficiency responses and its relationship to aluminum tolerance in wheat
A inibição do crescimento das culturas causada na condição de solos ácidos, devido principalmente pela deficiência de fósforo (P) e toxidez de alumínio (Al), é um sério problema para a produção agrícola. Em trigo, a cv. Toropi foi identificada como altamente tolerante ao Al e, também apresenta tolerância à presença de baixo P, quando compara
Publicado em: 2009
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7. Differential expression of metallothionein type-2 homologues in leaves and roots of Black pepper (Piper nigrum L)
Black pepper (Piper nigrum L.), member of the family Piperaceae is indigenous to India and is one of the most widely used spices in the world. In this paper we report the results of our attempts to identify a set of genes differentially expressed in the leaves of Piper nigrum, which could facilitate targeted engineering of this valuable crop. A PCR-based Sup
Genetics and Molecular Biology. Publicado em: 2008
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8. Análise da expressão gênica no dermatófito Trichophyton rubrum mimetizando a infecção in vitro: pH e diferentes fontes de carbono regulando genes / Analysis of gene expression in the dermatophyte Trichophyton rubrum during the mimetic infection in vitro: pH and carboun sources are regulating genes
Dermatophytes are a group of fungi filamentous that have the ability to invade keratinized substrates, causing dermatophytosis in humans and animals and only penetrate deeper if the host is immunocompromised. Trichophyton rubrum is an anthropophilic and cosmopolitan fungi, the most common agent of superficial mycoses, which uses cell components such as prote
Publicado em: 2008
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9. Gene expression analysis in sweet orange in response to Xanthomonas axonopodis pv. citri and Xanthomonas axonopodis pv. aurantifolii / Expressão diferencial de genes de laranja doce em resposta a infecção por Xanthomonas axonopodis pv. citri e axonopodis pv. aurantifolii
The most aggressive form of the citrus canker disease is caused by the bacteria Xanthomonas axonopodis pv. citri (Xac), which can infect all commercial varieties or species of citrus. In addition to Xac, Xanthomonas axonopodis pv. aurantifolii (Xaa) causes a weaker form of the disease, known as cancrose C, which is restricted to Mexican Lime (Citrus aurantif
Publicado em: 2008
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10. Identificação de genes diferencialmente expressos em tomateiro induzidos por ácido salicílico e por Fusarium oxysporum f. sp. lycopersici.
To identify tomato plant (Lycopersicon esculentum Mill), cv. BRH, genes which answer to plant pathogen Fusarium oxysporum f. sp. lycopersici and salicylic acid, the carrier molecule for activation of responses of plant defense, it was used the suppression subtractive hybridization (SSH) technique, from leaf cDNAs, 24h after salicylic acid, library denominate
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 14/06/2007
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11. Expressão genica diferencial em reticulocitos de pacientes com doença da hemoglobina H / Differential gene expression in reticulocytes of Hemoglobin H disease patients
Hb H disease results from the inactivation of three of the four alfa -globin genes normally present on diploid genome and it is characterized by a moderate to severe chronic hemolytic anemia. Patients usually present microcytosis, hypochromia and poikilocytosis, with 25 to 30% of Hb Bart?s (?4) at birth and 5 to 30% of Hb H (?4) in adult life. Although the m
Publicado em: 2007
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12. Molecular characterization of preferentially expressed genes in the yeast pathogenic phase of Paracoccidioides brasiliensis through the techniques of Macroarray and SSH (Suppression Subtraction Hybridization) / Caracterização molecular de genes preferencialmente expressos na fase leveduriforme patogênica de ´Paracoccidioides brasiliensis´ através das técnicas de ´Macroarray´ e de SSH (Suppression Substractive Hybridization)
Paracoccidioides brasiliensis, um fungo termodimórfico, é o agente causador da paracoccidioidomicose (PCM), a micose sistêmica prevalente da América Latina. A patogenicidade aparenta estar intimamente relacionada com a transição dimórfica da forma de micélio para a de levedura, que é induzida pela mudança da temperatura do ambiente pela temperatura
Publicado em: 2005