Supported Cultivation
Mostrando 13-24 de 43 artigos, teses e dissertações.
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13. Discursos do Progresso : Sorocaba e o fim das Feiras de Muares (1850-1900)
The mainly purpose of this research is to analyse the causes that have been taken Sorocaba mules fairs extinguishment, during the second half of nineteenth century. The castle trade had propiciated Sorocaba s development. During the months of january and may the annual castle fairs attracted several "men that taken care of the clattle" - tropeiros - who sold
Publicado em: 2003
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14. Cultivation of mycoplasmas in a modified tissue culture medium.
A new medium, which contained a chemically defined tissue culture base ("medium 199"), was developed for the cultivation of mycoplasmas. When supplemented with albumin, glucose, serum, and yeast extract, the new medium adequately supported the growth of Mycoplasma and Acholeplasma species.
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15. Determination of optimal liquid medium for enzyme expression by Helicobacter pylori.
AIMS: To determine the best medium for the growth and enzyme expression of Helicobacter pylori. METHODS: Twelve H pylori isolates from histologically confirmed infected patients were cultured on Brucella broth (BB), Brucella broth minus bisulphite (BLBB), and brain-heart infusion broth (BHIB), all supplemented with 5% (v/v) fetal calf serum. Growth rates and
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16. CULTIVATION OF LEPTOSPIRAE I. : Nutrition of Leptospira Canicola
Stalheim, O. H. V. (University of Wisconsin, Madison), and J. B. Wilson. Cultivation of leptospirae. I. Nutrition of Leptospira canicola. J. Bacteriol. 88:48–54. 1964.—The nutrition of Leptospira canicola was investigated by use of synthetic media of suitable ionic strength. At an incubation temperature of 30 C, the minimal components were calcium, iron,
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17. Growth of Campylobacter pylori in liquid media.
Until recently, broth cultivation techniques for Campylobacter pylori were unavailable. We developed a method to cultivate bacterial cells within 24 h in liquid media. Cultivation in broth depended on the adequate dispersion of appropriate gases. A static broth at 37 degrees C in a GasPak jar (BBL Microbiology Systems, Cockeysville, Md.) with a CampyPak (BBL
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18. Characteristics of a Hydrated, Alginate-Based Delivery System for Cultivation of the Button Mushroom
The production of the button mushroom Agaricus bisporus with mycelium-colonized alginate pellets as an inoculant of the growing medium was investigated. Pellets having an irregular surface and porous internal structure were prepared by complexing a mixture of 1% sodium alginate, 2 to 6% vermiculite, 2% hygramer, and various concentrations of Nutrisoy (soy pr
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19. Large-Scale Cultivation of Acidophilic Hyperthermophiles for Recovery of Secreted Proteins
An electric water heater was modified for large-scale cultivation of aerobic acidophilic hyperthermophiles to enable recovery of secreted proteins. Critical changes included thermostat replacement, redesign of the temperature control circuit, and removal of the cathodic anticorrosion system. These alterations provided accurate temperature and pH control. The
American Society for Microbiology.
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20. Chemically Defined Medium for Cultivation of Several Epiphytic and Phytopathogenic Spiroplasmas
A chemically defined medium, LD82, was formulated for in vitro cultivation of spiroplasmas. Medium LD82 supported good growth for four epiphytic and insect-pathogenic spiroplasmas, Spiroplasma floricola 23-6T, Spiroplasma sp. strain SR3, Spiroplasma sp. strain brevi, and Spiroplasma sp. strain AS576, and of the phytopathogenic spiroplasmas Spiroplasma citri
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21. Continuous Cultivation for Apparent Optimization of Defined Media for Cellulomonas sp. and Bacillus cereus
Steady-state continuous culture was used to optimize lean chemically defined media for a Cellulomonas sp. and Bacillus cereus strain T. Both organisms were extremely sensitive to variations in trace-metal concentrations. However, medium optimization by this technique proved rapid, and multifactor screening was easily conducted by using a minimum of instrumen
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22. Improved enrichment broth for cultivation of fastidious organisms.
An enrichment broth developed in our laboratory, fastidious broth (FB), was compared with two commercially available broth media, supplemented thioglycolate broth and enriched eugonic broth. FB supported the growth of a number of organisms that were not cultivatable in either of the other two media, including Corynebacterium jeikeium, Haemophilus influenzae,
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23. Molting, Ecdysis, and Reproduction of Trichinella spiralis Are Supported In Vitro by Intestinal Epithelial Cells
Trichinella spiralis is an obligate parasite of animals that has an unusual intracellular life cycle. Investigation of parasitism at the cellular and molecular levels has been challenging because of a shortage of tools for in vitro cultivation of T. spiralis. We have found that T. spiralis larvae molt, ecdyse, develop to adulthood, and reproduce when they ar
American Society for Microbiology.
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24. Cultivation of Pneumocystis carinii with WI-38 cells.
Pneumocystis carinii has been successfully cultured with WI-38 human embryonic lung fibroblasts. Inoculum was obtained from infected lungs of cortisone-treated Sprague-Dawley rats. Trophozoites reached peak numbers between days 4 and 8 and grew in two subcultures, but then proliferation ceased. If primary cultures were harvested and new medium were added, a