Substrates Af
Mostrando 1-12 de 19 artigos, teses e dissertações.
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1. Propriedades magnéticas de nanopartículas de ferro em substratos antiferromagnéticos
O efeito de tamanho finito nas propriedades magnéticas de sistemas de partículas ferromagnéticos é um tema recorrente. Um dos aspectos largamente investigados é o limite superparamagnético em que a temperatura desestabiliza a ordem magnética de partículas ferromagnéticas pequenas. Acima da temperatura de bloqueio o valor médio térmico do mom
Publicado em: 2007
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2. Estudo das bases moleculares da especificidade pelo substrato de uma beta-glicosidase (AF052729) de Spodoptera frugiperda / Study of the molecular basis of substrate specificity in a Spodoptera frugiperda beta-glycosidase (AF052729)
The Mr 50,000 Spodoptera frugiperda beta-glycosidase (Sfgli50) has active site amino acid residues that interact non-covalently with the substrate. These non-covalent interactions determine the enzymes specificity towards its substrates. This work aims to study the role of these amino acids and its interactions with the substrate in the specificity of Sfgl
Publicado em: 2006
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3. Alterações do perfil de lipoproteinas plasmaticas induzidas pela testosterona são moduladas pela expressão de proteina de transferencia de colesteril ester : estudos em camundongos transgenicos e controles
Sex honnones modify the plasma lipoprotein profile and hence the risk of atherosclerosis. ln this study we have investigated the effects of testosterone on plasma lipid and lipoprotein levels and associated enzymes. Transgenic mice (Tg) 1hat express a human cholesteryl ester transfer protein (CETP) minigene with natural flanking sequences and non transgernc
Publicado em: 2001
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4. Deposição e caracterização de filmes finos de We WSi e estudo da estabilidade termica do contato Schottky sobre GaAs
This dissertation presents a study of the physical and electrical characteristics of W and WSi x thin films as a function of the deposition parameters, done RF magnetron sputtering. Thin films of about 200 nm thickness were deposited on GaAs and SiO/2Si substrates. The depositions were done after a base-pressure of about 6.10-7 Torr was achieved. The deposit
Publicado em: 1992
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5. pBR322 plasmid DNA modified with 2-acetylaminofluorene derivatives: transforming activity and in vitro strand cleavage by the Escherichia coli uvrABC endonuclease.
Covalently closed circular plasmid DNA was treated with three reactive derivatives of 2-acetylaminofluorene: N-acetoxy-N-2-acetylaminofluorene (N-Aco-AAF), its 7-iodo derivative (N-Aco- AAIF ) and N-hydroxy-N-2-aminofluorene (N-OH-AF), and tested as substrates for the Escherichia coli uvrABC endonuclease and for transformation frequencies on wild-type, uvrA,
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6. Characterization of a U2AF-Independent Commitment Complex (E′) in the Mammalian Spliceosome Assembly Pathway
Early recognition of pre-mRNA during spliceosome assembly in mammals proceeds through the association of U1 small nuclear ribonucleoprotein particle (snRNP) with the 5′ splice site as well as the interactions of the branch binding protein SF1 with the branch region and the U2 snRNP auxiliary factor U2AF with the polypyrimidine tract and 3′ splice site. T
American Society for Microbiology.
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7. Near-precise interchromosomal recombination and functional DNA topoisomerase II cleavage sites at MLL and AF-4 genomic breakpoints in treatment-related acute lymphoblastic leukemia with t(4;11) translocation
We analyzed the der(11) and der(4) genomic breakpoint junctions of a t(4;11) in the leukemia of a patient previously administered etoposide and dactinomycin by molecular and biochemical approaches to gain insights about the translocation mechanism and the relevant drug exposure. The genomic breakpoint junctions were amplified by PCR. Cleavage of DNA sub
National Academy of Sciences.
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8. A Role for the Hsp40 Ydj1 in Repression of Basal Steroid Receptor Activity in Yeast
In addition to its roles in translocation of preproteins across membranes, Ydj1 facilitates the maturation of Hsp90 substrates, including mammalian steroid receptors, which activate transcription in yeast in a hormone-dependent manner. To better understand Ydj1's function, we have constructed and analyzed an array of Ydj1 mutants in vivo. Both the glucocorti
American Society for Microbiology.
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9. Pre-mRNA splicing in the absence of an SR protein RS domain
SR proteins are essential pre-mRNA splicing factors that act at the earliest stages of splice-site recognition and spliceosome assembly, as well as later in the splicing pathway. SR proteins consist of one or two RNA-recognition motifs and a characteristic arginine/serine-rich C-terminal RS domain. The RS domain, which is extensively phosphorylated, mediates
Cold Spring Harbor Laboratory Press.
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10. Degradation of 14C-Labeled Lignins and 14C-Labeled Aromatic Acids by Fusarium solani
Abilities of isolate AF-W1 of Fusarium solani to degrade the side chain and the ring structure of synthetic dehydrogenative polymerizates, aromatic acids, or lignin in sound wood were investigated under several conditions of growth substrate or basal medium and pH. Significant transformations of lignins occurred in 50 days in both unextracted and extracted s
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11. Combination of Two Activating Mutations in One HOG1 Gene Forms Hyperactive Enzymes That Induce Growth Arrest
Mitogen-activated protein kinases (MAPKs) play key roles in differentiation, growth, proliferation, and apoptosis. Although MAPKs have been extensively studied, the precise function, specific substrates, and target genes of each MAPK are not known. These issues could be addressed by sole activation of a given MAPK, e.g., through the use of constitutively act
American Society for Microbiology.
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12. Regulation of product chain length by isoprenyl diphosphate synthases
An analysis of the x-ray structure of homodimeric avian farnesyl diphosphate synthase (geranyltransferase, EC 2.5.1.10) coupled with information about conserved amino acids obtained from a sequence alignment of 35 isoprenyl diphosphate synthases that synthesize farnesyl (C15), geranylgeranyl (C20), and higher chain length isoprenoid diphosphates suggest
The National Academy of Sciences of the USA.