Subsite Specificity
Mostrando 1-12 de 26 artigos, teses e dissertações.
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1. Molecular bases of the specificity substrate of a β-glicodase / Bases moleculares da especificidade pelo substrato em β-glicosidases
The β-glycosidases of family 1 of the glycoside hydrolases (GH 1) are one of the most important groups of enzymes. These enzymes are involved in a high diversity of physiological functions. The main objective of this study was the analysis of the molecular bases of the specificity for substrate of a β-glycosidase from the larvae of Spodoptera frugi
Publicado em: 2009
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2. Purification, kinetics and physical-chemistry characterization of periplaneta americana, Tenebrio molitor and Diatraca saccharalis digestive chymotrypsin / Purificação, caracterização físico-químico e cinética das quimotripsinas digestivas de Periplaneta americana, Tenebrio molitor e Diatraea saccharalis.
Chymotrypsins (EC 3.4.21.1) are serine proteases with 245 amino acids disposed in two double ?-barrel domains, being the active site locate between these two domains. The specificity study of endopeptidases was facilitated by the substrate binding site concept that is the active site of these enzymes. However there is not much information about the subsites
Publicado em: 2006
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3. Insect serine-endopeptidases and plant-insect interactions / Serina endopeptidases de insetos e a interação inseto-planta
Serina endopeptidases de insetos, principalmente tripsinas e quimotripsinas, estão envolvidas na digestão inicial de proteínas. Genes codificadores para estas enzimas estão organizados em famílias multigênicas tendo expressão diferencial de acordo com a dieta do inseto, estando envolvidos no desenvolvimento de resistência a diferentes metabólitos se
Publicado em: 2004
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4. Caracterização das tripsinas de insetos / Characterization of insect trypsins
Tripsinas são enzimas comuns à maioria dos insetos e de fundamental importância para a digestão inicial de proteínas. Desta forma, tornam-se alvos importantes para orientar a construção de plantas transgênicas resistentes a insetos. As tripsinas são serina endopeptidases que clivam cadeias protéicas na porção carboxílica de resíduos de aminoác
Publicado em: 1999
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5. Origins of the specificity of tissue-type plasminogen activator.
The role of subsite interactions in defining the stringent substrate specificity of tissue-type plasminogen activator (t-PA) has been examined by using an fd phage library that displayed random hexapeptide sequences and contained 2 x 10(8) independent recombinants. Forty-four individual hexapeptides were isolated and identified as improved substrates for t-P
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6. Structural Basis for Broad Substrate Specificity in Higher Plant β-d-Glucan Glucohydrolases
Family 3 β-d-glucan glucohydrolases are distributed widely in higher plants. The enzymes catalyze the hydrolytic removal of β-d-glucosyl residues from nonreducing termini of a range of β-d-glucans and β-d-oligoglucosides. Their broad specificity can be explained by x-ray crystallographic data obtained from a barley β-d-glucan glucohydrolase in complex w
American Society of Plant Biologists.
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7. Portion-mixing peptide libraries of quenched fluorogenic substrates for complete subsite mapping of endoprotease specificity.
A solid-phase assay for the complete subsite mapping of the active site of endoproteases has been developed. A library of resin-bound protease substrates was synthesized both on kieselguhr-supported polyamide resin and on a polyethylene glycol-poly-(N,N-dimethylacrylamide) copolymer type of resin that allows proteases to diffuse into the interior and perform
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8. Structural Determinants of Autoproteolysis of the Haemophilus influenzae Hap Autotransporter▿
Haemophilus influenzae is a gram-negative bacterium that initiates infection by colonizing the upper respiratory tract. The H. influenzae Hap autotransporter protein mediates adherence, invasion, and microcolony formation in assays with respiratory epithelial cells and presumably facilitates colonization. The serine protease activity of Hap is associated wit
American Society for Microbiology (ASM).
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9. Synergy between adjacent zinc fingers in sequence-specific DNA recognition
Zif268-like zinc fingers are generally regarded as independent DNA-binding modules that each specify three base pairs in adjacent, but discrete, subsites. However, crystallographic evidence suggests that a contact also can occur from the second helical position of one finger to the subsite of the preceding finger. Here we show for the three-finger DNA-bindin
The National Academy of Sciences of the USA.
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10. Altered Substrate Specificity of Drug-Resistant Human Immunodeficiency Virus Type 1 Protease
Resistance to human immunodeficiency virus type 1 protease (HIV PR) inhibitors results primarily from the selection of multiple mutations in the protease region. Because many of these mutations are selected for the ability to decrease inhibitor binding in the active site, they also affect substrate binding and potentially substrate specificity. This work inv
American Society for Microbiology.
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11. Use of a zinc-finger consensus sequence framework and specificity rules to design specific DNA binding proteins.
We have designed three zinc-finger proteins with different DNA binding specificities. The design strategy combines a consensus zinc-finger framework sequence with previously characterized recognition regions such that the specificity of each protein is predictable. The first protein consists of three identical zinc fingers, each of which was expected to reco
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12. Probability model for molecular recognition in biological receptor repertoires: significance to the olfactory system.
A generalized phenomenological model is presented for stereospecific recognition between biological receptors and their ligands. We ask what is the distribution of binding constants psi(K) between an arbitrary ligand and members of a large receptor repertoire, such as immunoglobulins or olfactory receptors. For binding surfaces with B potential subsite and S