Strain Radial
Mostrando 37-48 de 72 artigos, teses e dissertações.
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37. Rescue of a paralyzed-flagella mutant of Chlamydomonas by transformation.
The biflagellate alga Chlamydomonas has been used extensively in the genetic and biochemical analysis of flagellar assembly and motility. We have restored motility to a paralyzed-flagella mutant of Chlamydomonas by transforming with the corresponding wild-type gene. A nitrate reductase-deficient paralyzed-flagella strain, nit1-305 pf-14, carrying mutations i
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38. Susceptibilities of Bordetella pertussis strains to antimicrobial peptides.
We examined the susceptibilities of Bordetella pertussis strains to several antimicrobial peptides by determining the concentration required to inhibit or kill 50% of the bacterial population. The peptides are ranked in decreasing potency as follows: cecropin B > cecropin A >> melittin > cecropin P1 > (ala8,13,18)-magainin II amide > mastoparan = defensin HN
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39. Further insights into the structural principles governing the function of articular cartilage.
A new experimental technique involving the observation of an artificial notch propagating through articular cartilage has been used to examine the biomechanical properties of this tissue. By predetermining both the orientation of the notch and its location with respect to the primary functional zones a more rigorous description of the structure/function rela
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40. Automated interpretation of disk diffusion antibiotic susceptibility tests with the radial profile analysis algorithm.
An original algorithm referred to as the radial profile analysis algorithm was implemented on a Macintosh Quadra 700 computer to provide an automatic determination of the inhibition zone diameters of antibiotic susceptibility tests performed with the disk diffusion method. After digitization of the petri plate image, each antibiotic disk is recognized and la
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41. Infection with an Extended-Spectrum β-Lactamase-Producing Strain of Serratia marcescens following Tongue Reconstruction
We report a case of postsurgical wound infection of polymicrobial etiology caused by Serratia marcescens and Pseudomonas aeruginosa following the use of a radial forearm free flap for oncological tongue reconstruction. S. marcescens was a producer of SHV-12 extended-spectrum β-lactamase (ESBL). This is the first report from India of this ESBL. S. marcescens
American Society for Microbiology.
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42. Evaluation of a radial immunodiffusion test with polysaccharide B antigen for diagnosis of bovine brucellosis.
A radial immunodiffusion (RID) test employing a polysaccharide antigen (poly B) was compared with tests currently used in the diagnosis of bovine brucellosis. Over 1,000 sera from vaccinated and infected cattle, all of which had been examined bacteriologically, were used to determine the sensitivity and specificity of the RID, card, Rivanol, and complement f
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43. Evaluation of the Single Radial Hemolysis Test for Measuring Hemagglutinin- and Neuraminidase-Specific Antibodies to H3N2 Influenza Strains and Antibodies to Influenza B
Antibodies to the H3 hemagglutinin of influenza A virus could be specifically measured by single radial hemolysis (SRH) when test antigens were recombinant viruses containing the relevant H3 hemagglutinin antigen and irrelevant Neq1 neuraminidase of A/equine/Prague/1/56 virus. Antibodies to influenza B virus could also be measured by the SRH technique. Antib
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44. Contribution of Proteus mirabilis urease to persistence, urolithiasis, and acute pyelonephritis in a mouse model of ascending urinary tract infection.
Proteus mirabilis, a significant cause of bacteriuria and acute pyelonephritis in humans, produces urease. This high-molecular-weight, multimeric, cytoplasmic enzyme hydrolyzes urea to ammonia and carbon dioxide. To assess the role of urease in colonization, urolithiasis, and acute pyelonephritis in an animal model of ascending urinary tract infection, we co
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45. Interaction of Mycoplasma hyopneumoniae with the porcine respiratory epithelium as observed by electron microscopy.
An in vivo-passaged strain of Mycoplasma hyopneumoniae attained viability titers of 10(6) to 10(8) color-changing units per mg of tissue in pig lungs and caused gross and histological pulmonic lesions. Mycoplasmas were readily located in the lumina of the respiratory tract by electron microscopy. In sections of tissue fixed in glutaraldehyde-osmium, the orga
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46. Susceptibility of Neisseria gonorrhoeae to protegrins.
We developed a sensitive and quantitative radial diffusion method to ascertain the susceptibility of six strains of Neisseria gonorrhoeae to antimicrobial peptides derived from mammalian leukocytes. The test organisms included the well-characterized serum-resistant FA19 and serum-sensitive F62 strains plus four antibiotic-resistant clinical isolates. Althoug
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47. Haemophilus ducreyi Is Susceptible to Protegrin
Protegrins, potent antimicrobial peptides found in porcine leukocytes, have activity against the sexually transmitted pathogens Neisseria gonorrhoeae, Chlamydia trachomatis, and human immunodeficiency virus type 1. We tested synthetic protegrin 1 (PG-1) for activity against nine isolates of Haemophilus ducreyi, the etiologic agent of chancroid. The test orga
American Society for Microbiology.
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48. Flagellar mutants of Chlamydomonas: Studies of radial spoke-defective strains by dikaryon and revertant analysis
The motility mutant of Chlamydomonas reinhardtii pf14 lacks radial spoke structures in its flagellar axonemes, and 12 proteins present in wild type are missing from a two-dimensional map (isoelectrofocusing/sodium dodecyl sulfate electrophoresis) of its 35S-labeled flagellar proteins. Six of these same proteins are missing in pf1, which lacks spoke-heads. To