Standard Lymphocyte Proliferation
Mostrando 1-12 de 17 artigos, teses e dissertações.
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1. A novel noninvasive method to detect rejection after heart transplantation
Prompt and accurate detection of rejection prior to pathological changes after organ transplantation is vital for monitoring rejections. Although biopsy remains the current gold standard for rejection diagnosis, it is an invasive method and cannot be repeated daily. Thus, noninvasive monitoring methods are needed. In this study, by introducing an IL-2 neutra
Braz J Med Biol Res. Publicado em: 2012-12
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2. Cytokine detection in lymphocyte cultures of chromium allergic patients / Detecção de citocinas em culturas de linfócitos em pacientes alérgicos ao cromo
A dermatite de contato alérgica decorre de uma reação imunológica, mediada por células T, contra um contactante em pessoas previamente sensibilizadas. O exame padrão ouro para o diagnóstico da DCA é o teste de contato (TC). Infelizmente, o TC demanda tempo, não é inócuo e tem algumas limitações. O teste de proliferação linfocitária (TPL) conv
Publicado em: 2010
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3. Suppression of in vitro lymphocyte proliferation in C57BL/10 ScN mice vaccinated with phase I Coxiella burnetii.
The effect of inactivated phase I and phase II Coxiella burnetii whole cell vaccine (WCV) on the response of murine spleen cells to mitogenic and antigenic stimuli was evaluated in C57BL/10 ScN endotoxin nonresponder mice with an in vitro lymphocyte proliferation assay. Intraperitoneal injection of phase I WCV into mice resulted in marked and persistent supp
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4. Comparison of Human Immunodeficiency Virus Antigens as Stimulants for Lymphocyte Proliferation Assays
CD4 proliferative responses to the human immunodeficiency virus (HIV) type 1 (HIV-1) p24 (gag) antigen inversely correlate with the plasma viral load in HIV-infected subjects who control viral replication without antiretroviral therapy. Use of a single HIV-1 protein to assess CD4 proliferative responses may not reflect the global response to this pathogen. W
American Society for Microbiology.
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5. Defective interfering influenza virus inhibits immunopathological effects of infectious virus in the mouse.
Mice inoculated intranasally with a lethal dose of standard influenza virus die with an immune-mediated pneumonia but are protected by coinoculation with defective interfering (DI) virus. Here we show that recruitment of immune cells into the infected lung is halved by treatment with DI virus although the CD4+/CD8+ cell ratio is not affected. Responsiveness
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6. Combined proliferating cell nuclear antigen and morphometric analysis in the diagnosis of cutaneous lymphoid infiltrates.
AIMS: To evaluate the use of morphometry in the diagnosis of benign and malignant cutaneous lymphoid infiltrates; and to determine whether the sensitivity of detection of cutaneous T cell lymphoma (CTCL) could be improved by selectively measuring cells expressing proliferating cell nuclear antigen (PCNA). METHODS: 44 archival biopsy specimens were studied. T
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7. Antibody-targeted interleukin 2 stimulates T-cell killing of autologous tumor cells.
A genetically engineered fusion protein consisting of a chimeric anti-ganglioside GD2 antibody (ch14.18) and interleukin 2 (IL2) was tested for its ability to enhance the killing of autologous GD2-expressing melanoma target cells by a tumor-infiltrating lymphocyte line (660 TIL). The fusion of IL2 to the carboxyl terminus of the immunoglobulin heavy chain di
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8. Targeted Disruption of the Inosine 5′-Monophosphate Dehydrogenase Type I Gene in Mice
Inosine 5′-monophosphate dehydrogenase (IMPDH) is the critical, rate-limiting enzyme in the de novo biosynthesis pathway for guanine nucleotides. Two separate isoenzymes, designated IMPDH types I and II, contribute to IMPDH activity. An additional pathway salvages guanine through the activity of hypoxanthine-guanine phosphoribosyltransferase (HPRT) to supp
American Society for Microbiology.
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9. A high-efficiency cloning system for single hapten-specific B lymphocytes that is suitable for assay of putative growth and differentiation factors.
Fluorescein (FLU)-specific murine splenic B lymphocytes from nonimmunized adult mice were prepared by the hapten-gelatin fractionation technique and cultured singly or in very small numbers in 10-microliters culture wells. Growth and differentiation to antibody-secreting status were promoted by polymeric FLU-conjugated antigens with or without added T-lympho
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10. Transport of recombinant human CD4-immunoglobulin G across the human placenta: pharmacokinetics and safety in six mother-infant pairs in AIDS clinical trial group protocol 146.
Recombinant CD4-immunoglobulin G (rCD4-IgG) is a 98-kDa human immunoglobulin-like protein that is produced by fusing the gp120 binding domain of CD4 to the Fc portion of the human IgG1 heavy chain. This hybrid molecule was given to human immunodeficiency virus (HIV)-infected pregnant women at the onset of labor by intravenous bolus at 1 mg/kg of body weight
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11. Induction of immunity and oral tolerance with polymorphic class II major histocompatibility complex allopeptides in the rat.
We studied the immunogenicity and tolerogenicity of class II major histocompatibility complex (MHC) allopeptides in the rat. Inbred LEW (RT1l) rats, used as responders, were immunized in the foot pad with a mixture of eight synthetic class II MHC allopeptides emulsified in complete Freund's adjuvant. These sequences represent the full-length second domain of
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12. Amplification of Migratory Inhibition Factor Production During the First 48 Hours of Exposure to Antigen
When lymphocyte-macrophage suspensions from sensitized animals are preincubated with specific antigen for 24 or more h, the following results are observed. (i) In a standard capillary macrophage migration test, there is complete inhibition of migration. (ii) When the preincubated cell suspension is mixed in varying proportions with a similar suspension from