Stained Glass
Mostrando 25-36 de 56 artigos, teses e dissertações.
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25. Different quantal responses within single frog neuromuscular junctions.
At frog neuromuscular junctions spontaneous miniature end-plate potentials (m.e.p.p.s) were recorded from several isolated spots within single synapses. This was done by consecutively placing an extracellular glass micro-electrode (focal electrode) at different recording sites, while the intracellular electrode remained in one place. After each set of record
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26. Evidence for Separate Adhesion Mechanisms for Hydrophilic and Hydrophobic Surfaces in Vibrio proteolytica
The proteolytic enzymes pronase, trypsin, and chymotrypsin and the surfactant Triton X-100 inhibited attachment of Vibrio proteolytica to the hydrophobic substratum polystyrene by >97%. These treatments had no effect on attachment to hydrophilic substrata such as glass or tissue culture dishes. Both pronase and Triton X-100 effected the removal of previously
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27. Confirmation of Legionella pneumophila cultures with a fluorescein-labeled monoclonal antibody.
We compared a fluorescein-labeled monoclonal antibody directed against an outer membrane protein of Legionella pneumophila (Genetic Systems Corp. [GSC], Seattle, Wash.) with a similarly labeled polyclonal reagent (L. pneumophila serogroups 1 to 6, poly; BioDx, Inc., Denville, N.J.) for the confirmation of L. pneumophila isolates grown in culture. Duplicate s
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28. Blood-borne tissue factor: Another view of thrombosis
Arterial thrombosis is considered to arise from the interaction of tissue factor (TF) in the vascular wall with platelets and coagulation factors in circulating blood. According to this paradigm, coagulation is initiated after a vessel is damaged and blood is exposed to vessel-wall TF. We have examined thrombus formation on pig arterial media (which contains
The National Academy of Sciences.
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29. Sedimentation Counting and Morphology of Mycoplasma
Clark, Harold W. (The George Washington University School of Medicine, Washington, D.C.). Sedimentation counting and morphology of Mycoplasma. J. Bacteriol. 90:1373–1386. 1965.—The sedimentation technique for counting viral particles was applied to the quantitation and morphological identification of Mycoplasma in broth cultures. Mycoplasma, apparently i
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30. Sensitive PCR-Restriction Fragment Length Polymorphism Assay for Detection and Genotyping of Giardia duodenalis in Human Feces
An assay that uses heminested PCR-restriction fragment length polymorphism analysis for the detection and genotyping of Giardia duodenalis on the basis of polymorphism in the triose phosphate isomerase (tpi) gene was developed. This assay was evaluated with DNA extracted from purified parasite material, bacterial cultures, whole human feces containing G. duo
American Society for Microbiology.
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31. Orcein staining of hepatitis B antigen in paraffin sections of liver biopsies.
Liver biopsies from 97 hepatitis B antigen (HBsAG)-positive patients were stained by a modified orcein method described by Shikata et al (1974) in order to detect the antigen in liver tissue. The results were consitently negative in acute hepatitis, but positive in nearly two-thirds of biopsies from 53 patients with chronic liver disease. The distribution of
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32. Fibrillar Array in the Cell Wall of a Gliding Filamentous Cyanobacterium
The cell walls of a number of filamentous, gliding cyanobacteria of the genus Oscillatoria were examined by transmission electron microscopy of ultrathin sections, of freeze-etched replicas, and of whole cells crushed between glass slides and negatively stained. All three techniques revealed the presence of a highly ordered array of parallel fibrils, seen in
American Society for Microbiology.
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33. Mesenchymal precursor cells in the blood of normal individuals
Mesenchymal precursor cells found in the blood (BMPCs) of normal persons adhere to plastic and glass and proliferate logarithmically in DMEM-20% fetal calf serum (FCS) without growth factors. They form cells with fibroblast-like and stromal morphology, which is not affected by eliminating CD34, CD3, or CD14 cells. Osteogenic supplements (dexamethasone, ascor
BioMed Central.
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34. Precapsular film on the aging human lens: precursor of pseudoexfoliation?
In many older patients we observed a layer of subtle opacification on the anterior lens capsule, appearing as a ground glass film biomicroscopically. This precapsular film (PCF) could be uniform but often had radial grey lines in the mid zone, holes in the paracentral region, and was occasionally rolled up in strings. Lens capsular material obtained at catar
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35. Surface morphology and function of human pulmonary alveolar macrophages from smokers and non-smokers.
Pulmonary alveolar macrophages were obtained by saline lavage from 23 healthy male volunteers--10 non-smokers and 13 cigarette smokers. Lavage produced three times as many alveolar macrophages in smokers than in non-smokers. When macrophages from smokers and from non-smokers were incubated in vitro, more cells from smokers adhered to glass, spread out, and s
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36. Cytoskeletal Effects Induced by Pet, the Serine Protease Enterotoxin of Enteroaggregative Escherichia coli
We have previously described enteroaggregative Escherichia coli (EAEC) strains that induce cytotoxic effects on T84 cells, ligated rat ileal loops, and human intestine in culture. Such strains secrete a 104-kDa protein termed Pet (for plasmid-encoded toxin). We have also shown previously that the Pet toxin induces rises in short-circuit current and decreases
American Society for Microbiology.