Spinner Flasks
Mostrando 1-8 de 8 artigos, teses e dissertações.
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1. Desenvolvimento de um bioprocesso para expansão de células mesenquimais estromais multipotentes em microcarregadores / Bioprocess development for expansion of mesenchymal stem cells on microcarriers.
As células mesenquimais estromais multipotentes (CMM) são na atualidade uma fonte atrativa para aplicações na engenharia de tecidos e na terapia celular. Devido à baixa disponibilidade nos tecidos (0,01%-0,0005%) e às elevadas doses necessárias para uma infusão (aproximadamente 106 células/Kg paciente) tornou-se necessário o desenvolvimento de tecn
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 04/05/2012
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2. Successful scale-up of human embryonic stem cell production in a stirred microcarrier culture system
Future clinical applications of human embryonic stem (hES) cells will require high-yield culture protocols. Currently, hES cells are mainly cultured in static tissue plates, which offer a limited surface and require repeated sub-culturing. Here we describe a stirred system with commercial dextran-based microcarriers coated with denatured collagen to scale-up
Brazilian Journal of Medical and Biological Research. Publicado em: 2009-06
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3. Caracterização metabólica e cinética do cultivo de três hibridomas para produção de imunoglobulinas com especificidade e antígenos eritrocitários para uso hemoterápico.
In the last two decades mammalian cell cultures have been broadly used for the industrial production of pharmaceutical, proteins, in particular the monoclonal antibodies by cultures of murine hybrid cells (hybridoma). The development of bioprocess for industrial scale of monoclonal antibodys (MAbs) depends on the determination and optimization of several phy
Publicado em: 2007
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4. Proliferation of rat Pneumocystis carinii on cells sheeted on microcarrier beads in spinner flasks.
A method of growing rat Pneumocystis carinii with human embryonic lung fibroblasts (HEL-299 cells) sheeted onto microcarrier beads has been developed. This method allows production of large quantities of P. carinii organisms with very little contamination of host cells. A fivefold increase in the numbers of organisms was achieved, as determined by organism c
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5. Production of high levels of human leukocyte interferon from a continuous human myeloblast cell culture.
A myeloblast cell line has proved to be an excellent source of human leukocyte interferon. These cells, primed with interferon and induced with Sendai virus, produced optimal levels of human leukocyte interferon. The cells grew readily in spinner flasks and in medium containing horse serum. Interferon production over several months yielded an average titer o
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6. Growth and Metabolism of L Cells in a Chemically Defined Medium in a Controlled Environment Culture System: I. Effects of O2 Tension on L-Cell Cultures
Six water-jacketed 500-ml Bellco spinner flasks were equipped to monitor and control environmental variables to study their effects on the growth and metabolism of mammalian cells. Studies with automated control of pO2 levels of l-cell cultures, grown at pH 6.9 ± 0.1, showed that dissolved O2 tensions of ca. 9% were optimal for cell growth. At pO2 values of
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7. Pneumocystis carinii pneumonia in scid mice induced by viable organisms propagated in vitro.
Pneumocystis carinii pneumonia remains a major cause of morbidity and mortality in human immunodeficiency virus-infected individuals, despite the widespread use of prophylaxis and the development of new chemotherapeutic agents. The study of P. carinii and of pulmonary host defenses directed against it has been limited by lack of reliable, reproducible method
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8. Characterization of human hybridomas secreting antibody to tetanus toxoid.
We have selected a thioguanine-resistant lymphoblastoid cell line (LTR228) that forms human-human hybrids with high efficiency. Fusions with peripheral B cells consistently yield one colony per 10(5) cells plated. To produce antitetanus monoclonal antibodies, we withdrew blood from persons who had recently received booster injections of tetanus toxoid. T cel