Species Specific Primer
Mostrando 1-12 de 311 artigos, teses e dissertações.
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1. A Rapid and Reliable Method for Molecular Detection of Fusarium guttiforme, the Etiological Agent of Pineapple Fusariosis
Abstract Pineapple (Ananas comosus var. comosus) fusariosis is an economically important fungal disease affecting the plant and its fruit. A rapid and reliable diagnosis is the base of integrated disease management practices. Fusariosis has resulted in quarantines for pineapple products in Central America, Africa and Asia. Difficulties diagnosing and correc
Braz. arch. biol. technol.. Publicado em: 25/11/2019
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2. Phenotypic and molecular characterization of apple (Malus × domestica Borkh) genetic resources in Greece
ABSTRACT: The phenotypic and genetic analysis of apple (Malus × domestica Borkh) genotypes is essential for breeding species. Information on the morphology and genetic structure of apple offers significant help for germplasm maintenance and selection of suitable material to breed superior cultivars. This study shows the results of an investigation on the mo
Sci. agric. (Piracicaba, Braz.). Publicado em: 2018-12
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3. Improved reverse transcription-polymerase chain reaction assay for the detection of flaviviruses with semi-nested primers for discrimination between dengue virus serotypes and Zika virus
BACKGROUND The genus Flavivirus includes a variety of medically important viruses, including dengue virus (DENV) and Zika virus (ZIKV), which are most prevalent in Brazil. Because the clinical profile of patients affected by different DENV serotypes or ZIKV may be similar, the development of new methods that facilitate a rapid and accurate diagnosis is cru
Mem. Inst. Oswaldo Cruz. Publicado em: 26/02/2018
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4. Molecular diagnosis of cryptococcal meningitis in cerebrospinal fluid: comparison of primer sets for Cryptococcus neoformans and Cryptococcus gattii species complex
Aim:This study evaluated the use of polymerase chain reaction for cryptococcal meningitis diagnosis in clinical samples.Materials and methods:The sensitivity and specificity of the methodology were evaluated using eight Cryptococcus neoformans/C. gattiispecies complex reference strains and 165 cere- brospinal fluid samples from patients with neurological d
Braz J Infect Dis. Publicado em: 2015-02
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5. Rapid PCR-based assay for Sclerotinia sclerotiorum detection on soybean seeds
Caused by Sclerotinia sclerotiorum, white mold is an important seed-transmitted disease of soybean (Glycine max). Incubation-based methods available for the detection and quantification of seed-borne inoculum such as the blotter test, paper roll and Neon-S assay are time-consuming, laborious, and not always sensitive. In this study, we developed and evaluate
Sci. agric. (Piracicaba, Braz.). Publicado em: 2015-02
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6. Molecular characterization of lactobacilli isolated from fermented idli batter
Lactic acid bacteria are non pathogenic organism widely distributed in nature typically involved in a large number of spontaneous food fermentation. The purpose of this study was to characterize the bacteriocinogenic lactobacilli from fermented idli batter which can find application in biopreservation and biomedicine. Eight most promising lactobacilli were c
Braz. J. Microbiol.. Publicado em: 2013-12
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7. Molecular identification of Lactobacillus spp. associated with puba, a Brazilian fermented cassava food
Puba or carimã is a Brazilian staple food obtained by spontaneous submerged fermentation of cassava roots. A total of 116 lactobacilli and three cocci isolates from 20 commercial puba samples were recovered on de Man, Rogosa and Sharpe agar (MRS); they were characterized for their antagonistic activity against foodborne pathogens and identified taxonomicall
Braz. J. Microbiol.. Publicado em: 19/03/2013
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8. Toxigenic profile and AFLP variability of Alternaria alternata and Alternaria infectoria occurring on wheat
The objectives of this study were to evaluate the ability to produce alternariol (AOH), alternariol monomethyl ether (AME) and tenuazonic acid (TA) by A. alternata and A. infectoria strains recovered from wheat kernels obtained from one of the main production area in Argentina; to confirm using AFLPs molecular markers the identify of the isolates up to speci
Braz. J. Microbiol.. Publicado em: 2013
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9. Variation in extragenic repetitive DNA sequences in Pseudomonas syringae and potential use of modified REP primers in the identification of closely related isolates
In this study, Pseudomonas syringe pathovars isolated from olive, tomato and bean were identified by species-specific PCR and their genetic diversity was assessed by repetitive extragenic palindromic (REP)-PCR. Reverse universal primers for REP-PCR were designed by using the bases of A, T, G or C at the positions of 1, 4 and 11 to identify additional polymor
Genetics and Molecular Biology. Publicado em: 05/07/2012
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10. The relationship between genetic variability and the susceptibility of Biomphalaria alexandrina snails to Schistosoma mansoni infection
In the present study, Biomphalaria snails collected from five Egyptian governorates (Giza, Fayoum, Kafr El-Sheikh, Ismailia and Damietta), as well as reference control Biomphalaria alexandrina snails from the Schistosome Biological Supply Center (SBSC) (Theodor Bilharz Research Institute, Egypt), were subjected to species-specific polymerase chain reaction (
Memórias do Instituto Oswaldo Cruz. Publicado em: 2012-05
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11. Caracterización y variabilidad genética de Colletotrichum gloeosporioides sensu lato en plantaciones de palma aceitera (Elaeis guineensis Jacq.) en Venezuela
Characterization was performed on the causal agent of anthracnose affecting leaves, rachis and fruits of oil palms in the southern region of Maracaibo Lake (Venezuela). Morphological tests included colony growth rate, diameter, color and type of mycelium, as well as the shape and size of conidia. Molecular characterization included specific PCR tests, ITS rD
Tropical Plant Pathology. Publicado em: 2012-04
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12. Natural infection of several Coffea species and hybrids and Psilanthus ebracteolatus by the coffee ringspot virus (CoRSV)
Coffee ringspot is a minor coffee disease caused by the nuclear type of Brevipalpus mite-transmitted virus, Coffee ringspot virus (CoRSV). Recently outbreaks of the disease in some growing regions of the state of Minas Gerais, Brazil, were registered with qualitative and quantitative yield losses. Coffea arabica was the only species registered as natural hos
Scientia Agricola. Publicado em: 2011-08