Solid Phase Peptide Synthesis
Mostrando 13-24 de 72 artigos, teses e dissertações.
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13. DNA Display III. Solid-Phase Organic Synthesis on Unprotected DNA
DNA-directed synthesis represents a powerful new tool for molecular discovery. Its ultimate utility, however, hinges upon the diversity of chemical reactions that can be executed in the presence of unprotected DNA. We present a solid-phase reaction format that makes possible the use of standard organic reaction conditions and common reagents to facilitate ch
Public Library of Science.
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14. Chemoselective one-step purification method for peptides synthesized by the solid-phase technique.
The specific reaction between SH and iodoacetamide groups has been explored as the basis of an affinity-type purification procedure for peptides synthesized by the solid-phase technique. For this affinity-type purification procedure, we synthesized an SH precursor reagent bearing an acid-labile S-protecting group, pMB-SCH2CONHCH2CH2-SO2CH2CH2OCO2pNP (compoun
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15. Mechanisms and prevention of trifluoroacetylation in solid-phase peptide synthesis
A novel mechanism for trifluoroacetylation in solid-phase peptide synthesis, independent of the coupling step, has been elucidated. It involves the presence of trifluoroacetoxymethyl groups on the resin support, which react with resin-bound amines by an intersite nucleophilic reaction. The trifluoroacetoxymethyl groups are generated from preexisting hydroxym
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16. Conformational influences of glycosylation of a peptide: A possible model for the effect of glycosylation on the rate of protein folding
Improved strategies for synthesis make it possible to expand the range of glycopeptides available for detailed conformational studies. The glycopeptide 1 was synthesized using a new solid phase synthesis of carbohydrates and a convergent coupling to peptide followed by deprotection. Its conformational properties were subjected to NMR analysis and compar
The National Academy of Sciences of the USA.
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17. Synthetic peptide vaccine design: synthesis and properties of a high-density multiple antigenic peptide system.
A convenient and versatile approach to the direct synthesis of a peptide-antigen matrix by the solid-phase method is described. The approach is called the multiple antigen peptide system (MAP) and it utilizes a simple scaffolding of a low number of sequential levels (n) of a trifunctional amino acid as the core matrix and 2n peptide antigens to form a macrom
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18. The synthesis of polyamide-oligonucleotide conjugate molecules.
We have developed methods for the synthesis of peptide-oligodeoxyribonucleotide conjugate molecules in particular, and polyamide-oligonucleotide conjugates in general. Synthesis is carried out by a solid-phase procedure and involves the assembly of a polyamide on the solid support, conversion of the terminal amino group to a protected primary aliphatic hydro
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19. SOLID PHASE SYNTHESIS OF A 42-RESIDUE FRAGMENT OF STAPHYLOCOCCAL NUCLEASE: PROPERTIES OF A SEMISYNTHETIC ENZYME
The polypeptide corresponding to the amino acid sequence from residue 6 through 47 in staphylococcal nuclease has been synthesized by the solid-phase method. The synthetic product closely resembles the corresponding native polypeptide in both physical and chemical properties. The synthetic peptide may be recombined with the complimentary native peptide compr
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20. Synthesis of a 19-residue peptide with alamethicin-like activity.
This paper describes the chemical synthesis of a compound with voltage-gating characteristics similar to those observed in nerve membranes. For alamethicin (ALA), a natural antibiotic that induces such properties in lipid bilayer membranes, there are two proposed structures, one a cyclic and the other an open chain peptide. The open chain sequence (ALA-o) pr
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21. Solid-phase synthesis of cecropin A and related peptides.
Cecropin A, a 37-residue antibacterial peptide amide, was synthesized by the solid-phase method. It was shown to be homogeneous and totally indistinguishable from natural cecropin A by chemical and physical criteria, as well as by its antibacterial activity against several Gram-positive and Gram-negative organisms. The synthetic material was also used to est
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22. Rapid synthesis of oligodeoxyribonucleotides. II. Machine-aided solid-phase syntheses of two nonanucleotides and an octanucleotide.
Preparation of the two nonanucleotides, d(pG-G-A-G-G-A-G-A-A) and d(pT-T-C-T-G-T-T-G-A), and the octanucleotide, d(pT-G-G-C-C-C-A-T) is described by a rapid solid-phase method on a polyamide support. The syntheses were carried out using a modified solid-phase peptide synthesiser and required one day for each nucleotide addition. A microparticulate anion-exch
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23. Liquid-phase combinatorial synthesis.
A concept termed liquid-phase combinatorial synthesis (LPCS) is described. The central feature of this methodology is that it combines the advantages that classic organic synthesis in solution offers with those that solid-phase synthesis can provide, through the application of a linear homogeneous polymer. To validate this concept two libraries were prepared
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24. Solid-phase synthesis of a nucleopeptide from the linking site of adenovirus-2 nucleoprotein, -Ser(p5'CATCAT)-Gly-Asp-. Convergent versus stepwise strategy.
The synthesis of a nucleopeptide with the sequence -Ser(p5'CATCAT)-Gly-Asp- has been undertaken by either convergent or stepwise solid-phase strategies, both of which use base-labile permanent protecting groups. The coupling of phosphitylated protected peptides onto oligonucleotide-resins did not afford the desired nucleopeptide, which was nevertheless obtai