Sodium Pyruvate
Mostrando 13-24 de 136 artigos, teses e dissertações.
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13. Growth Properties of Rhodospirillum rubrum Mutants and Fermentation of Pyruvate in Anaerobic, Dark Conditions1
Mutant C and G1 were obtained earlier from Rhodospirillum rubrum S1 during growth in the dark under strict anaerobic conditions in medium containing sodium pyruvate. Mutant C and mutant G1 grew in the dark with generation times of 5.8 h and 4.6 h, respectively. Mutant C cells grew equally well when switched between anaerobic (dark or light) or aerobic, dark
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14. Effect of ferrous sulfate, sodium metabisulfite, and sodium pyruvate on survival of Campylobacter jejuni.
A combination of ferrous sulfate, sodium metabisulfite and sodium pyruvate, incorporated in solid medium, maintained the characteristic morphology, motility, and viability of six isolates of Campylobacter jejuni stored at room temperature and 4 degrees C for up to 20 and 30 days, respectively, under normal atmospheric conditions.
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15. Reduction of nicotinamide adenine dinucleotide by pyruvate:lipoate oxidoreductase in anaerobic, dark-grown Rhodospirillum rubrum mutant C.
Cell extracts from fermentatively grown Rhodospirillum rubrum reduced about 80 nmol of nicotinamide adenine dinucleotide (NAD) per mg of protein per min under anaerobic conditions with sodium pyruvate. The reaction was specific for pyruvate and NAD; NAD phosphate was not reduced. Results indicated that pyruvate-linked NAD reduction occurred via pyruvate:lipo
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16. Growth Supplements for Helicobacter pylori
The growth response of Helicobacter pylori in broth was determined in the presence of ferrous sulfate, sodium pyruvate, and mucin (porcine stomach). The addition of either ferrous sulfate and sodium pyruvate or mucin to brain heart infusion broth with 7% horse serum (BHI-HS) enhanced the growth of H. pylori. The best growth of strain NB2-1, which was the slo
American Society for Microbiology.
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17. Enhanced recovery of injured Escherichia coli by compounds that degrade hydrogen peroxide or block its formation.
Escherichia coli LSUFS was injured either by freezing at -10 degrees C or by heating at 57 degrees C for 12 min. Surviving cells were recovered on nonselective tryptone-glucose extract agar and selective violet red bile agar supplemented with compounds that degrade hydrogen peroxide or block its formation. Various concentrations of the following compounds we
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18. Carbon dioxide fixation and mixotrophic metabolism by strain DCB-1, a dehalogenating anaerobic bacterium.
Fixation by strain DCB-1 of CO2 carbon into cell material and organic acids occurred during growth on pyruvate both with and without thiosulfate. By using sodium [14C]bicarbonate and sodium [2-14C]pyruvate, the isotopic composition of products and cells was investigated. Up to 70% of cell carbon was derived from CO2. CO2 carbon was also incorporated into suc
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19. Cultivation of Parasitic Leptospires: Effect of Pyruvate
Sodium pyruvate (100 μg/ml) is a useful addition to the Tween 80-albumin medium for the cultivation of parasitic serotypes. It is most effective in promoting growth from small inocula and growth of the nutritionally fastidious serotypes.
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20. Role of Pyruvate and S-Adenosylmethionine in Activating the Pyruvate Formate-Lyase of Escherichia coli1
The pyruvate formate-lyase activity of extracts of Escherichia coli is stimulated and the dilution effect is abolished by the addition of pyruvate to the extract. The activity can be purified fourfold from pyruvate-supplemented extracts by isoelectric precipitation under anaerobic conditions. The activity of extracts not supplemented with pyruvate has been s
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21. Subunit stoichiometry and molecular weight of the pyruvate dehydrogenase multienzyme complex from Escherichia coli.
The molar ratio of the component enzymes of the pyruvate dehydrogenase multienzyme complex from Escherichia coli was found to be 1.8:1.7:1[pyruvate decarboxylase (E1):dihydrolipoyl transacetylase (E2):dihydrolipoyl dehydrogenase (E3)]. This ratio was determined by measuring the Coomassie blue staining of the constituent enzymes after sodium dodecyl sulfate/p
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22. Effect of Mixed Culture on Nitrosomonas europaea Simulated by Uptake and Utilization of Pyruvate
Clark, Connie (University of Minnesota, Minneapolis), and E. L. Schmidt. Effect of mixed culture on Nitrosomonas europaea simulated by uptake and utilization of pyruvate. J. Bacteriol. 91:367–373. 1966.—Contamination of a 5-liter culture of Nitrosomonas europaea resulted in enhanced nitrite formation as compared with pure cultures. The dominant contamina
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23. Characterization of the specific pyruvate transport system in Escherichia coli K-12.
A mutant of Escherichia coli K-12 lacking pyruvate dehydrogenase and phosphoenolpyruvate synthase was used to study the transport of pyruvate by whole cells. Uptake of pyruvate was maximal in mid-log phase cells, with a Michaelis constant for transport of 20 microM. Pretreatment of the cells with respiratory chain poisons or uncouplers, except for arsenate,
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24. THE CENTRAL ROLE OF PYRUVATE IN THE STIMULATION OF SODIUM TRANSPORT BY ALDOSTERONE*