Site Oriented
Mostrando 37-48 de 235 artigos, teses e dissertações.
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37. Structure-Function Relationships in Lactate Dehydrogenase
The binding of coenzyme and substrate are considered in relation to the known primary and tertiary structure of lactate dehydrogenase (EC 1.1.1.27). The adenine binds in a hydrophobic crevice, and the two coenzyme phosphates are oriented by interactions with the protein. The positively charged guanidinium group of arginine 101 then folds over the negatively
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38. Orientation of functional activating regions in the Escherichia coli CRP protein during transcription activation at class II promoters.
At class II CRP-dependent promoters the DNA site for CRP overlaps the DNA site for RNA polymerase, covering the -35 region. Transcription activation at class II CRP- dependent promoters requires a contact between an activating region in the upstream subunit of the bound CRP dimer and a contact site in the C-terminal domain of the alpha-subunit of RNA polymer
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39. Molecular basis of cooperative DNA bending and oriented heterodimer binding in the NFAT1—Fos–Jun—ARRE2 complex
Cooperative DNA binding by transcription factors that bind to separate recognition sites is likely to require bending of intervening sequences and the appropriate orientation of transcription factor binding. We investigated DNA bending in complexes formed by the basic region–leucine zipper domains of Fos and Jun with the DNA binding region of nuclear facto
The National Academy of Sciences.
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40. RNA polymerase as a repressor of transcription in the hut(P) region of mutant Klebsiella aerogenes histidine utilization operons.
Mutants of Klebsiella aerogenes able to express the hutUH operon in the absence of positive effectors were isolated and characterized. These mutations improve the hutUH promoter (PUH) by changing the -10 region to match the consensus sequence more closely. These mutations also affect another, oppositely oriented promoter in this region, PC. Although the muta
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41. Catalytic residues of gamma delta resolvase act in cis.
The resolvase protein of the gamma delta transposon is a site-specific recombinase that acts by a concerted break-and-join mechanism. To analyse the role of individual resolvase subunits in DNA strand cleavage, we have directed the binding of catalytic mutants to specific recombination crossover sites or half-sites. Our results demonstrate that the resolvase
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42. The active site architecture of Pisum sativum β-carbonic anhydrase is a mirror image of that of α-carbonic anhydrases
We have determined the structure of the β–carbonic anhydrase from the dicotyledonous plant Pisum sativum at 1.93 Å resolution, using a combination of multiple anomalous scattering off the active site zinc ion and non-crystallographic symmetry averaging. The mol– ecule assembles as an octamer with a novel dimer of dimers of dimers arrangement. Two dist
Oxford University Press.
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43. Recognition of DNA by ω protein from the broad-host range Streptococcus pyogenes plasmid pSM19035: analysis of binding to operator DNA with one to four heptad repeats
pSM19035-encoded ω protein forms a dimer (ω2) that binds to a set of 7-bp repeats with sequence 5′-NATCACN-3′. Upon binding to its cognate sites, ω2 regulates transcription of genes required for copy number control and stable inheritance of plasmids, and promotes accurate plasmid segregation. Protein ω2 binds poorly to one heptad but the affinity to
Oxford University Press.
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44. Nucleotide sequence and expression of the gene for the site-specific integration protein from bacteriophage HP1 of Haemophilus influenzae.
The nucleotide sequence of the leftmost 2,363 base pairs of the HP1 genome, which includes the attachment site (attP) and the integration region, was determined. This sequence contained an open reading frame encoding a 337-residue polypeptide, which is a member of the integrase family of site-specific recombination proteins as judged by sequence comparison.
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45. Rapid Site-Directed Mutagenesis Using Two-PCR-Generated DNA Fragments Reproducing the Plasmid Template
We describe a new rapid and efficient polymerase chain reaction (PCR)-based site-directed mutagenesis method. This procedure is effective with any plasmid and it employs four oligonucleotide primers. One primer contains the desired mutation, the second is oriented in the opposite direction (one of these two primers should be phosphorylated), and the third an
Hindawi Publishing Corporation.
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46. Generation of Food-Grade Recombinant Lactic Acid Bacterium Strains by Site-Specific Recombination
The construction of a delivery and clearing system for the generation of food-grade recombinant lactic acid bacterium strains, based on the use of an integrase (Int) and a resolvo-invertase (β-recombinase) and their respective target sites (attP-attB and six, respectively) is reported. The delivery system contains a heterologous replication origin and antib
American Society for Microbiology.
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47. Activation of Chi, a recombinator, by the action of an endonuclease at a distant site.
Chi is an Escherichia coli recombinator specific to the recBC pathway. In phage lambda, its activity is dependent on its orientation with respect to the orientation of the chromosome packaging origin, cos [Kobayashi, I., Murialdo, H., Crasemann, J.M., Stahl, M. M. & Stahl, F. W. (1982) Proc. Natl. Acad. Sci. USA 79, 5981-5985]. Chi in an inactive state is ac
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48. The adenovirus E3 14.5-kilodalton protein, which is required for down-regulation of the epidermal growth factor receptor and prevention of tumor necrosis factor cytolysis, is an integral membrane protein oriented with its C terminus in the cytoplasm.
We previously reported that the adenovirus type 5 E3 14.5-kilodalton protein (14.5K) forms a complex with E3 10.4K and that both proteins are required to down-regulate the epidermal growth factor receptor in adenovirus-infected human cells. Both proteins are also required to prevent cytolysis by tumor necrosis factor of most mouse cell lines infected by aden