Site Directed Mutagenesis
Mostrando 1-12 de 2316 artigos, teses e dissertações.
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1. Genetic engineering of Theileria parva lactate dehydrogenase gene: a new anti-theilerial target
ABSTRACT: Theileria parva is the causative agent of East Coast Fever (ECF), a tick borne disease, which results in major economic losses in cattle. Major problems in dealing with this illness are the high cost of drugs, development of resistance, and absence of effective vaccines. Thus, exploiting new targets for cost effective and higher therapeutic value d
Pesq. Vet. Bras.. Publicado em: 2018-05
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2. Modulation of the prostaglandin-endoperoxide synthase 2 gene expression by variant haplotypes: influence of the 3′-untranslated region
The inducible inflammatory enzyme cycloxigenase-2 is up-regulated in cancer, and favors tumor progression. Cycloxigenase-2 is encoded by the prostaglandin-endoperoxide synthase 2 (PTGS2) gene, which presents sequence variations in the promoter region (PR) and in the 3′-untranslated region (3′-UTR). Different PR (rs689465, rs689466, rs20417) and 3′-UTR
Braz J Med Biol Res. Publicado em: 30/11/2017
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3. Stress-related hormone norepinephrine induces interleukin-6 expression in GES-1 cells
In the current literature, there is evidence that psychological factors can affect the incidence and progression of some cancers. Interleukin 6 (IL-6) is known to be elevated in individuals experiencing chronic stress and is also involved in oncogenesis and cancer progression. However, the precise mechanism of IL-6 induction by the stress-related hormone nor
Braz J Med Biol Res. Publicado em: 2014-02
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4. Estudo da atividade biológica do motivo ECD do domínio tipo-desintegrina da botropasina / Biological activity study of ecd motif of disintegrinlike domain of bothropasin
As integrinas são receptores de superfície celular capazes de mediar a adesão celular e a agregação plaquetária, que embora façam parte de importantes processos fisiológicos no organismo também estão relacionados a patologias como tumorogênese, metástase e trombose. Deste modo as integrinas constituem importantes alvos no desenvolvimento de droga
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 10/05/2011
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5. Mutants of common bean alpha-amylase inhibitor-2 as an approach to investigate binding specificity to alpha-amylases.
Despite the presence of a family of defense proteins, Phaseolus vulgaris can be attacked by bruchid insects resulting in serious damage to stored grains. The two distinct active forms of a-amylase inhibitors, a-AI1 and a-AI2, in P. vulgaris show different specificity toward a-amylases. Zabrotes subfasciatus a-amylase is inhibited by a-AI2 but not by a-AI1. I
Pesquisa Agropecuária Brasileira. Publicado em: 2011
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6. Mapeamento de potenciais interações envolvidas na agregação e na formação de fibrilas amilóides em apomioglobina / Mapping of potential interactions involved in apomyoglobin aggregation and amyloid fibrils formation
Proteínas enoveladas incorretamente, com freqüência levam à formação de agregados fibrilares contendo extensas estruturas em folha-β, comumente denominadas de fibrilas amilóides. A hipótese acerca da capacidade de formar fibrilas amilóides com estruturas idênticas e ricas em estruturas beta, ser uma propriedade genérica de toda proteína, apo
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 04/05/2010
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7. Characterization of the interdependency between residues that bind the substrate in a β-glycosidase
The manner by which effects of simultaneous mutations combine to change enzymatic activity is not easily predictable because these effects are not always additive in a linear manner. Hence, the characterization of the effects of simultaneous mutations of amino acid residues that bind the substrate can make a significant contribution to the understanding of t
Brazilian Journal of Medical and Biological Research. Publicado em: 2010-01
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8. Influence of the polymorphisms of the α-major regulatory element HS-40 on in vitro gene expression
The α-MRE is the major regulatory element responsible for the expression of human α-like globin genes. It is genetically polymorphic, and six different haplotypes, named A to F, have been identified in some population groups from Europe, Africa and Asia and in native Indians from two Brazilian Indian tribes. Most of the mutations that constitute the α-MRE
Brazilian Journal of Medical and Biological Research. Publicado em: 31/07/2009
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9. Molecular bases of the specificity substrate of a β-glicodase / Bases moleculares da especificidade pelo substrato em β-glicosidases
The β-glycosidases of family 1 of the glycoside hydrolases (GH 1) are one of the most important groups of enzymes. These enzymes are involved in a high diversity of physiological functions. The main objective of this study was the analysis of the molecular bases of the specificity for substrate of a β-glycosidase from the larvae of Spodoptera frugi
Publicado em: 2009
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10. Bases moleculares do efeito do pH na atividas catalítica de duas lisozimas digestivas de Musca domestica (Diptera) / Molecular basis of the pH effect on the catalytic activity of two digestive lysozymes from Musca domestica (Diptera)
Lysozymes are enzymes that are part of the defence mechanism against bacteria, however lysozymes with digestive function are also found in the digestive tract of vertebrates and in the insect midgut. The digestive lysozymes from insects are c type, so they share similar structural and mechanistic characteristics with hen egg-white lysozyme (HEWL). However, t
Publicado em: 2008
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11. MAPEAMENTO E DELEÇÃO DE EPÍTOPOS LINEARES DE LINFÓCITOS B EM PROTEÍNAS DO VÍRUS DA SÍNDROME RESPIRATÓRIA E REPRODUTIVA DOS SUÍNOS PARA A PRODUÇÃO DE UMA VACINA DIFERENCIAL / MAPPING AND DELETION OF B-CELL LINEAR EPITOPES IN PROTEINS OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS FOR THE PRODUCTION OF A DIFFERENTIAL VACCINE
Porcine reproductive and respiratory syndrome virus (PRRSV) was isolated for the first time in 1991 and since then it has been associated with significant economic losses to the pig industry worldwide. Although vaccination against PRRSV is widely used, an important advance would be the development of marker vaccines allowing serologic discrimination between
Publicado em: 2008
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12. Production and characterization of Mycoplasma hyopneumoniae recombinant proteins with potential for use in immunodiagnosis and vaccination / Produção e caracterização de proteínas recombinantes de Mycoplasma hyopneumoniae com potencial para uso em imunodiagnóstico e vacinação
Mycoplasma hyopneumoniae é o agente etiológico da pneumonia enzoótica suína (PES), uma doença respiratória responsável por significativas perdas econômicas. Vacinas comerciais são mundialmente utilizadas no controle desta doença, porém proporcionam apenas proteção parcial. Além disso, são caras devido ao crescimento fastidioso do M. hyopneumon
Publicado em: 2008