Single Size Of Box
Mostrando 1-12 de 22 artigos, teses e dissertações.
-
1. Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation
A bioprocess was developed for extracellular MAP production from Streptomyces gedanensis by solid-state fermentation. Response surface methodology of Box Behken Design was performed to evaluate the interaction effects of most significant variables {inoculum size, (NH4)2SO4 concentration, MgSO4.7H2O and tryptone) on MAP production after the single parameter o
Braz. arch. biol. technol.. Publicado em: 2014-04
-
2. Metaheurística tabu aplicada ao problema de carregamento de contêiner com caixas idênticas
Intermodal transport, that is, the integration between different transport modes was facilitated by the use of containers. The stowage devices in the breakdown of the cargo is an important logistics activity, since the total volume actually used can affect significantly the cost of transporting the products. This approach addresses the Container Loading Prob
Publicado em: 2009
-
3. Effect of periodic box size on aqueous molecular dynamics simulation of a DNA dodecamer with particle-mesh Ewald method.
The particle-mesh Ewald (PME) method is considered to be both efficient and accurate for the evaluation of long-range electrostatic interactions in large macromolecular systems being studied by molecular dynamics simulations. This method assumes "infinite" periodic boundary conditions resembling the symmetry of a crystal environment. Can such a "solid-state"
-
4. The RNA polymerase I transcription factor xUBF contains 5 tandemly repeated HMG homology boxes.
The RNA polymerase I transcription factor UBF has been identified in human, mouse, rat and Xenopus and the primary structure of the human protein has been determined. Human UBF was shown to contain four tandem homologies to the folding domains of the HMG1 and 2 proteins and hence to belong to a previously unrecognised family of 'HMG-box' transcription factor
-
5. Isolation and characterization of maize cDNAs encoding a high mobility group protein displaying a HMG-box.
cDNAs encoding a nonhistone chromosomal high mobility group (HMG) protein corresponding to the animal HMG1 family were isolated from a maize cDNA library using an immunoscreening approach. The cDNAs revealed an open reading frame of 471 base pairs together with 413 base pairs of flanking region, in agreement with the size of mRNA detected by Northern analysi
-
6. In vitro accurate initiation of transcription on the adenovirus type 2 IVa2 gene which does not contain a TATA box.
In vitro initiation of transcription has been studied on the IVa2 gene of the adenovirus type 2 ( Ad2 ) which is expressed at intermediate time after infection. This gene does not contain a TATA box around 30 bp upstream from the cap site. By analyses of the in vitro runoff products by size and by single strand nuclease protection mapping, it was concluded t
-
7. Rearrangement of structured RNA via branch migration structures catalysed by the highly related DEAD-box proteins p68 and p72
RNA helicases, like their DNA-specific counterparts, can function as processive enzymes, unwinding RNA with a defined step size in a unidirectional fashion. Recombinant nuclear DEAD-box protein p68 and its close relative p72 are reported here to function in a similar fashion, though the processivity of both RNA helicases appears to be limited to only a
Oxford University Press.
-
8. U20, a novel small nucleolar RNA, is encoded in an intron of the nucleolin gene in mammals.
We have found that intron 11 of the nucleolin gene in humans and rodents encodes a previously unidentified small nucleolar RNA, termed U20. The single-copy U20 sequence is located on the same DNA strand as the nucleolin mRNA. U20 RNA, which does not possess a trimethyl cap, appears to result from intronic RNA processing and not from transcription of an indep
-
9. Rat glucokinase gene: structure and regulation by insulin.
The glucokinase gene is 15.5-kilobases long, appears to be present as a single copy, and contains 10 exons that range in size from 96 to 977 base pairs. The transcription start site was located 127 nucleotides upstream from the translation initiation codon. The 5' flanking DNA contains several regions similar to defined promoter elements. These include a pro
-
10. Molecular cloning and expression of the mouse translation initiation factor eIF-1A.
Prior to determining the molecular basis for the transient increase in expression of eIF-1A during the 2-cell stage of the pre-implantation mouse embryo, we determined the sequence of full-length cDNA and defined properties of the genomic organization of the mouse eIF-1A gene. Northern blot analysis distinguishes three transcripts in mouse liver of 2.8, 2.2
-
11. Characterization and purification of H1TF2, a novel CCAAT-binding protein that interacts with a histone H1 subtype-specific consensus element.
Definition of mechanisms regulating human histone H1 gene transcription during the cell cycle requires the isolation and biochemical characterization of protein factors which interact with specific promoter elements. Two distinct binding activities have been identified in nuclear extracts from HeLa cells and mapped within a 180-base-pair (bp) region of a cel
-
12. Structural and Biophysical Characterization of the Proteins Interacting with the Herpes Simplex Virus 1 Origin of Replication
The C terminus of the herpes simplex virus type 1 origin-binding protein, UL9ct, interacts directly with the viral single-stranded DNA-binding protein ICP8. We show that a 60-amino acid C-terminal deletion mutant of ICP8 (ICP8ΔC) also binds very strongly to UL9ct. Using small angle x-ray scattering, the low resolution solution structures of UL9ct alone, in
American Society for Biochemistry and Molecular Biology.