Single Domain Particles
Mostrando 1-12 de 91 artigos, teses e dissertações.
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1. Evaluation of the anisotropy field for fine-particle systems from low-field thermomagnetic curves
A method for evaluating the temperaturedependent magnetic anisotropy field from thermomagnetic curves for Stoner–Wohlfarth-like systems is proposed. It allows the anisotropy parameters of a sample with unknown spontaneous magnetization to be obtained by using magnetic fields considerably lower than the anisotropy field.
Publicado em: 2011
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2. Interaction fields evaluation in fine particle systems
A computer simulation model for estimation of the interaction fields in fine particle systems has been developed. The method uses the experimental 8Mα(H) plot, constructed from the Fourier description of the initial magnetization curve and the hysteresis loop, and the 8M(H) plot, obtained from the remanence curves. The dependencies of the interaction fields
Publicado em: 2011
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3. Thermodynamic properties of small magnetic particles
We investigate the equilibrium magnetic properties of a simple cubic small ferromagnetic particle under an external magnetic field. Although the particle is small, it can not be considered as a single-domain unit. The magnetic moments are represented by unitary spin vectors and we consider ferromagnetic interactions between nearest-neighbor spins. The coupli
Brazilian Journal of Physics. Publicado em: 2006-09
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4. Murine Leukemia Virus Nucleocapsid Mutant Particles Lacking Viral RNA Encapsidate Ribosomes
A single retroviral protein, termed Gag, is sufficient for assembly of retrovirus-like particles in mammalian cells. Gag normally selects the genomic RNA of the virus with high specificity; the nucleocapsid (NC) domain of Gag plays a crucial role in this selection process. However, encapsidation of the viral RNA is completely unnecessary for particle assembl
American Society for Microbiology.
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5. Polyploid measles virus with hexameric genome length
Particles of most virus species accurately package a single genome, but there are indications that the pleomorphic particles of parainfluenza viruses incorporate multiple genomes. We characterized a stable measles virus mutant that efficiently packages at least two genomes. The first genome is recombinant and codes for a defective attachment protein with an
Oxford University Press.
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6. Coronavirus Particle Assembly: Primary Structure Requirements of the Membrane Protein
Coronavirus-like particles morphologically similar to normal virions are assembled when genes encoding the viral membrane proteins M and E are coexpressed in eukaryotic cells. Using this envelope assembly assay, we have studied the primary sequence requirements for particle formation of the mouse hepatitis virus (MHV) M protein, the major protein of the coro
American Society for Microbiology.
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7. In vitro assembly of virus-like particles with Rous sarcoma virus Gag deletion mutants: identification of the p10 domain as a morphological determinant in the formation of spherical particles.
Retroviruses are unusual in that expression of a single protein, Gag, leads to budding of virus-like particles into the extracellular space. We have developed conditions under which virus-like particles are formed spontaneously in vitro from fragments of Rous sarcoma virus (RSV) Gag protein purified after expression in Escherichia coli. The CA-NC fragment of
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8. Receptor activity of rotavirus nonstructural glycoprotein NS28.
Rotavirus morphogenesis involves the budding of subviral particles through the rough endoplasmic reticulum (RER) membrane of infected cells. During this process, particles acquire the outer capsid proteins and a transient envelope. Previous immunocytochemical and biochemical studies have suggested that a rotavirus nonstructural glycoprotein, NS28, encoded by
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9. Mutations in the Carboxyl-Terminal Domain of the Small Hepatitis B Virus Envelope Protein Impair the Assembly of Hepatitis Delta Virus Particles
The carboxyl-terminal domain of the small (S) envelope protein of hepatitis B virus was subjected to mutagenesis to identify sequences important for the envelopment of the nucleocapsid during morphogenesis of hepatitis delta virus (HDV) virions. The mutations consisted of carboxyl-terminal truncations of 4 to 64 amino acid residues and small combined deletio
American Society for Microbiology.
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10. Virus-Like Particles of a Fish Nodavirus Display a Capsid Subunit Domain Organization Different from That of Insect Nodaviruses
The structure of recombinant virus-like particles of malabaricus grouper nervous necrosis virus (MGNNV), a fish nodavirus isolated from the grouper Epinephelus malabaricus, was determined by electron cryomicroscopy (cryoEM) and three-dimensional reconstruction at 23-Å resolution. The cryoEM structure, sequence comparison, and protein fold recognition analys
American Society for Microbiology.
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11. A Single-Amino-Acid Substitution of a Tyrosine Residue in the Rubella Virus E1 Cytoplasmic Domain Blocks Virus Release
Rubella virus particles, consisting of a nucleocapsid surrounded by a lipid envelope in which two virus-encoded glycoproteins E1 and E2 are embedded, assemble on intracellular membranes and are secreted from cells, possibly via the cellular secretory pathway. We have recently demonstrated that the cytoplasmic domain of E1 (residues 469 to 481, KCLYYLRGAIAPR)
American Society for Microbiology.
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12. PPPYEPTAP Motif Is the Late Domain of Human T-Cell Leukemia Virus Type 1 Gag and Mediates Its Functional Interaction with Cellular Proteins Nedd4 and Tsg101
The human T-cell leukemia virus type 1 (HTLV-1) Gag polyprotein contains two adjacent proline-rich motifs (sequence PPPYEPTAP) in the C terminus of the matrix domain. Proline-to-alanine mutations were introduced into either or both motifs of HTLV-1 to determine the effect on the release of HTLV-1 virus-like particles from 293T cells. The release of both sing
American Society for Microbiology.