Silk Fibroin
Mostrando 1-12 de 35 artigos, teses e dissertações.
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1. IMPACT OF TEMPERATURE ON THE STABILITY OF SILKWORM COCOON FIBERS
In this study raw and processed silkworm (Bombyx mori) cocoons were investigated using confocal Raman spectroscopy in conjunction with differential scanning calorimetry (DSC) in order to examine the effect of temperature on the molecular conformation of silk fiber structures. Using DSC, a broad endothermic curve was observed at moderate temperatures, sheddin
Quím. Nova. Publicado em: 05/12/2019
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2. On air blowing direction in the blown bubble-spinning
This paper studies the effect of airflow direction on morphology of the nanofibers obtained by the blown bubble-spinning, a kind of Bubbfil spinning processes. Regenerated silk fibroin is used in the experiment, and the results indicate that nanofibers with random array structure can be obtained when the blowing air direction is perpendicular to the directio
Matéria (Rio J.). Publicado em: 2014-12
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3. Fabrication and characterization of nanofibrous scaffold developed by electrospinning
Electrospinning has been recognized as an efficient technique for the forming of polymer nanofibers. Silk fibroin (SF) nanofibers were electrospun from SF solution using trifluoroacetic acid solution as a solvent. In the present work, we have systematically evaluated the effects of instrument parameters, including applied voltage, tip-target distance, soluti
Materials Research. Publicado em: 23/09/2011
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4. Preparação, avaliação físico-química e biológica in vitro de pericárdio bovino conjugado com fibroína de seda/quitosana via liofilização e irradiação / Preparation, physico-chemical and biological in vitro evaluation of bovine pericardium combined with silk fibroin/chitosan via freeze-drying and irradiation
Neste trabalho, a irradiação por feixe de elétrons foi estudada como uma ferramenta para a reticulação do tecido de pericárdio bovino (PB). A conjugação de quitosana e fibroína de seda no tecido de PB liofilizado também foi objeto de estudo deste trabalho. Para isto, amostras de PB foram liofilizadas e irradiadas em um acelerador de elétrons utili
Publicado em: 2010
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5. Obtenção e caracterização de blendas e compósitos poliméricos de fibroína de seda e alginato de sódio / Production and characterization of polymeric blends and composites of silk fibroin and sodium alginate
A utilização de polímeros de fontes renováveis vem sendo amplamente estudada tanto no campo biomédico quanto nas indústrias de embalagens. Polímeros naturais são, em geral, biodegradáveis, biocompatíveis e podem ser obtidos a custos relativamente baixos. Biopolímeros como o alginato de sódio e a fibroína de seda vêm se destacando como potenciai
Publicado em: 2010
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6. Effect of freezing methods on the properties of lyophilized porous silk fibroin membranes
Silk fibroin is a fibrous protein that has been extensively studied for application in the biomedical field, and has been used as a scaffold for bone tissue engineering. Biomaterials made of proteins are prone to physical and chemical degradation during storage; lyophilization, a drying method that consists of freezing and drying steps, is known to promote m
Materials Research. Publicado em: 2009-06
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7. Estudo da esterilização e da calcificação in vitro de membranas de fibroina de seda para a aplicação como biomaterial / Study of sterization and in vitro calcification of silk fibroin membranes for biomaterial application
Silk fibroin has been widely explored for many biomedical applications, due to its biocompatibility, biodegradability and minimal inflammatory reactions. Sterilization is a fundamental step in biomaterials processing, and it must not alter in a negative way the functionality of medical devices. On the applicability of fibroin as a biomaterial, recent studies
Publicado em: 2009
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8. Hidrogeis e filmes de fibroina de seda para fabricação ou recobrimento de biomateriais / Silk fibroin hydrogels and films for biomaterials production or coating
Hidrogéis e filmes de fibroína de seda foram preparados e caracterizados com o objetivo de avaliar sua potencial aplicação no campo de biomateriais. Hidrogéis foram obtidos durante a etapa de diálise da solução de fibroína de seda e suas propriedades físicas, químicas, citotoxicidade e potencial de calcificação in vitro foram determinados. Esses
Publicado em: 2009
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9. Preparation and characterization of silk fibroin membranes to use as biomaterial / Obtenção e caracterização de membranas de fibroina de seda para aplicação como biomaterial
Fibroin is a fibrous protein, also found in silk. Recently, several researchers have investigated silk fibroin as a promising resource for biotechnology and biomedical materiaIs due to its mechanical resistance, biocompatibility and minimal inflammatory reaction. Based on these characteristics, other applications have been proposed, as wound protection, subs
Publicado em: 2005
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10. 13C NMR of Nephila clavipes major ampullate silk gland.
The major ampullate glands of the spider Nephila clavipes contain approximately 0.2 microliter each of a highly concentrated (approximately 50%) solution of silk fibroin. Therefore, the reservoir of silk in these glands presents an ideal opportunity to observe prefolded conformations of a protein in its native state. To this end, the structure and conformati
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11. Discontinuous translation of silk fibroin in a reticulocyte cell-free system and in intact silk gland cells.
Silk fibroin mRNA was translated in a rabbit reticulocyte cell-free system. Addition of tRNA from silk glands was essential for complete translation of the fibroin polypeptide. (Mr approximately 400,000). Synthesis of full-sized product took at least 85 min. In addition to full-size product, a large number of smaller polypeptides were observed upon analysis
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12. Natural fibroin genes purified without using cloning procedures from fibroin-producing and -nonproducing tissues reveal indistinguishable structure and function.
Natural fibroin genes were purified from total DNA extracted from the fibroin-producer cells (posterior silk gland) and -nonproducer cells (middle silk gland or pupa) by two cycles of CsCl/actinomycin D centrifugation followed by sucrose density gradient centrifugation. Purity of the final samples was greater than 14%. DNA sequences of these natural genes be