Short Tandem Repeats Str
Mostrando 13-24 de 24 artigos, teses e dissertações.
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13. Aplicação de microssatélites (STR) de bovinos em animais de raças zebuínas
O gado zebu (Bos indicus) é predominante em todo o mundo. Atualmente, no Brasil, o rebanho de bovinos e zebuínos é composto por aproximadamente 170 milhões de indivíduos, no qual, 80% são zebuínos ou animais de raças cruzadas com zebu. O principal objetivo deste estudo foi determinar a possibilidade da utilização de marcadores moleculares de bovino
Publicado em: 2007
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14. Extinction of canid populations by inbreeding depression under stochastic environments in Southwestern Goiás State: a simulation study
A frequently addressed question in conservation biology is what is the chance of survival for a population for a given number of years under certain conditions of habitat loss and human activities. This can be estimated through an integrated analysis of genetic, demographic and landscape processes, which allows the prediction of more realistic and precise mo
Genetics and Molecular Biology. Publicado em: 2007
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15. The minissatelite frequency in a Brazilian population and its usefulness for the human identification / A frequencia de regiões de minissatelites em uma população brasileira e sua utilidade para a identificação humana
A detecção do polimorfismo do DNA analisando microssatélites (STR - Short Tandem Repeats) ou minissatélites (VNTR - Variable Numbers of Tandem Repeats) pelas técnicas de PCR (Polymerase Chain Reaction) ou RFLP (Restriction Fragment Lenght Polymorphism) causou um grande impacto e revolucionou a ciência forense. Hoje se pode, com grande precisão, exclui
Publicado em: 2007
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16. Tipagem de DNA extraído de lâmina de esfregaço vaginal em casos suspeitos de estupro
Na suspeita de casos de estupro, é necessária a realização de exames para constatar a prática do abuso sexual com penetração. Com esse objetivo, nos serviços de medicina legal, é realizada, entre outros exames, a feitura de esfregaços vaginais com a transferência do material biológico para lâmina. Neste trabalho, são descritos dois casos forens
Sao Paulo Medical Journal. Publicado em: 2004-04
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17. Estrutura genÃtica da populaÃÃo pernambucana pela determinaÃÃo do polimorfismo de 5 locos de STRs
154 indivÃduos nÃo relacionados da populaÃÃo do Estado de Pernambuco (Nordeste do Brasil) foram estudados quanto ao polimorfismo de cinco locos de short tandem repeats (STR). A metodologia usada foi PCR-PAGE seguida de coloraÃÃo com nitrato de prata. Os alelos mais freqÃentes foram: 15 (0.3403), 11 (0.3264), 13 (0.2718), 15 (0.1753) e 30 (0.2247), par
Publicado em: 2003
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18. Characterization of Human Cytomegalovirus Strains by Analysis of Short Tandem Repeat Polymorphisms
Human cytomegalovirus (HCMV) strains display genetic polymorphisms, and these polymorphisms can be analyzed to study viral transmission and pathogenesis. Recently, short tandem repeat (STR) length polymorphisms have been identified in the HCMV genome. We assessed the utility of STRs in characterizing HCMV strains and found that a multiplexed PCR assay using
American Society for Microbiology.
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19. Rapid and accurate characterisation of short tandem repeats by MALDI-TOF analysis of endonuclease cleaved RNA transcripts
We describe the application of matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) for the characterisation of short tandem repeat (STR) sequences by the analysis of endonuclease cleaved RNA transcripts. Several simple bovine STR loci as well as interrupted and compound microsatellites were chosen as model loci to eval
Oxford University Press.
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20. Rapid, high fidelity analysis of simple sequence repeats on an electronically active DNA microchip
We describe a method for the discrimination of short tandem repeat (STR) alleles based on active microarray hybridization. An essential factor in this method is electronic hybridization of the target DNA, at high stringency, in <5 min. High stringency is critical to avoid slippage of hybrids along repeat tracts at allele-specific test sites in the array. The
Oxford University Press.
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21. Analysis of short tandem repeat polymorphisms by electrospray ion trap mass spectrometry
The application of electrospray ionization (ESI) ion trap mass spectrometry (MS) to the analysis of short tandem repeats (STRs or microsatellites) is described. Several equine dinucleotide STR loci were chosen as a model system to evaluate ESI ion trap as a routine instrument for rapid and reliable genoytping. With the use of specific primers STR loci were a
Oxford University Press.
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22. DNA typing in thirty seconds with a microfabricated device
We report the development of a practical ultrafast allelic profiling assay for the analysis of short tandem repeats (STRs) by using a highly optimized microfluidic electrophoresis device. We have achieved baseline-resolved electrophoretic separations of single-locus STR samples in 30 sec. Analyses of PCR samples containing the four loci CSF1PO, TPOX, THO1, a
The National Academy of Sciences of the USA.
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23. Vntr Allele Frequency Distributions under the Stepwise Mutation Model: A Computer Simulation Approach
Variable numbers of tandem repeats (VNTRs) are a class of highly informative and widely dispersed genetic markers. Despite their wide application in biological science, little is known about their mutational mechanisms or population dynamics. The objective of this work was to investigate four summary measures of VNTR allele frequency distributions: number of
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24. Multiple Polymorphic Loci for Molecular Typing of Strains of Mycobacterium leprae
The need for molecular tools for the differentiation of isolates of Mycobacterium leprae, the organism that causes leprosy, is urgent in view of the continuing high levels of new case detection, despite years of aggressive chemotherapy and the consequent reduction in the prevalence of leprosy. The slow onset of leprosy and the reliance on physical examinatio
American Society for Microbiology.