Serine Proteinases
Mostrando 1-12 de 103 artigos, teses e dissertações.
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1. Subproteome of Lachesis muta rhombeata venom and preliminary studies on LmrSP-4, a novel snake venom serine proteinase
Abstract Background: Lachesis muta rhombeata is one of the venomous snakes of medical importance in Brazil whose envenoming is characterized by local and systemic effects which may produce even shock and death. Its venom is mainly comprised of serine and metalloproteinases, phospholipases A2 and bradykinin-potentiating peptides. Based on a previously report
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 15/04/2019
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2. Kazal-type serine proteinase inhibitors in the midgut of Phlebotomus papatasi
Sandflies (Diptera: Psychodidae) are important disease vectors of parasites of the genus Leishmania, as well as bacteria and viruses. Following studies of the midgut transcriptome of Phlebotomus papatasi, the principal vector of Leishmania major, two non-classical Kazal-type serine proteinase inhibitors were identified (PpKzl1 and PpKzl2). Analyses of expres
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-09
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3. Isolated biomolecules of pharmacological interest in hemostasis from Cerastes cerastes venom
Biomolecules from Cerastes cerastes venom have been purified and characterized. Two phospholipases isolated from Cerastes cerastes venom share 51% of homology. CC2-PLA2 exhibits antiplatelet activity that blocks coagulation. CCSV-MPase, a non-hemorrhagic Zn2+-metalloproteinase, significantly reduced the plasmatic fibrinogen level and hydrolyzes only its Bβ
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 01/05/2013
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4. In vivo evaluation of homeostatic effects of Echis carinatus snake venom in Iran
Background The venom of the family Viperidae, including the saw-scaled viper, is rich in serine proteinases and metalloproteinases, which affect the nervous system, complementary system, blood coagulation, platelet aggregation and blood pressure. One of the most prominent effects of the snake venom of Echis carinatus (Ec) is its coagulation activity, used f
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 27/02/2013
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5. A pipeline to study structural interactions among Spodoptera frugiperda serine proteinases and plant proteinase inhibitors.
We propose here a computational biology pipeline to identify and analyze possible structural determinants that could explain some level of insensitivity by S. frugiperda serine proteinases (SPs) against plant PIs observed in a real time PCR experiment.
CONGRESSO BRASILEIRO DE GENÉTICA. Publicado em: 2011
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6. Caracterização estrutural da interação de serino proteinases de Spodoptera frugiperda (Lepidoptera: Noctuidae) e inibidores de proteinases de plantas / Structural characterisation of Spodoptera frugiperda (Lepidoptera: Noctuidae) serine proteinase interactions with plant proteinase inhibitors.
As plantas desenvolveram diferentes mecanismos para reduzir o ataque de insetos, incluindo compostos protéicos de defesa, como os inibidores de proteinases (IPs). Os insetos, ao longo da evolução, desenvolveram estratégias para superar as barreiras defensivas das plantas, permitindo a sua alimentação e desenvolvimento, como a super expressão de genes
Publicado em: 2011
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7. Anti-oxidative and proteolytic activities and protein profile of laticifer cells of Cryptostegia grandiflora, Plumeria rubra and Euphorbia tirucalli
In this study, proteins extracted from laticifer cells of three plants were examined by electrophoresis, mass spectrometry (MALDI-TOF) and characterized in respect of proteolytic, chitinolytic and anti-oxidative activities by means of zymography and colorimetric assays. Acidic proteins with molecular masses between 12.5 and 74.5 kDa predominated in laticifer
Brazilian Journal of Plant Physiology. Publicado em: 2010
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8. Potential of laticifer fluids for inhibiting Aedes aegypti larval development: evidence for the involvement of proteolytic activity
It has been shown previously that the laticifer fluid of Calotropis procera (Ait.) R.Br. is highly toxic to the egg hatching and larval development of Aedes aegypti L. In the present study, the larvicidal potential of other laticifer fluids obtained from Cryptostegia grandiflora R.Br., Plumeria rubra L. and Euphorbia tirucalli L. was evaluated. We attempted
Memórias do Instituto Oswaldo Cruz. Publicado em: 2009-09
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9. Avaliação da atividade de metaloproteinases e serinoproteinases na saliva de pacientes com doença pulmonar obstrutiva crônica. / Evaluation of the activity of metaloproteinases and serinoproteinases in the saliva of patients with pulmonary illness obstrutiva chronicle. / Evaluation of the activity of metaloproteinases and serinoproteinases in the saliva of patients with pulmonary illness obstrutiva chronicle. / Avaliação da atividade de metaloproteinases e serinoproteinases na saliva de pacientes com doença pulmonar obstrutiva crônica.
The present study was conducted to evaluate the activity of enzymatic components of saliva and possible correlations with clinical parameters in patients with COPD. The sample consisted of 30 individuals, 20 individuals with COPD and 10 controls. The clinical parameters of each individual were obtained by spirometry and 6 minute walk distance. Enzyme activit
Publicado em: 2009
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10. Purification and characterization of the aprotinin from porcine lung. / Purificação e caracterização da aprotinina obtida de pulmão suíno.
Aprotinin, an acid stable serine proteinase inhibitor with a molecular mass of 7 kDa, is used as a reagent or drug. The purification of the aprotinin from porcine lungs was the main objective of this work. Three procedures were used. The first one utilized the trypsin-agarose column. The tangential ultra filtration and trypsin-Sepharose column were used in t
Publicado em: 2008
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11. Estudo da ação do inibidor de proteinase de Adenanthera pavonina sobre o desenvolvimento e atividade das proteinases intestinais de lagartas de Diatraea suchharalis (FABR., 1794) / Study of proteinase inhibitor action from Adenanthera pavonina on the development and midgut proteinase activities of the Diatraea sachharalis larvae (FABR., 1794)
Phytophatogous insects and other animals use digestive enzymes, such as amylases and proteinases to process the nutrients obtained from the plants necessary for their development. The sugarcane borer Diatraea saccharalis is the major pest of sugarcane in Brazil and other South American countries. Plants synthesize a variety of molecules, including proteinase
Publicado em: 2008
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12. Efeitos de inibidores de proteinases de soja em organismos não-alvo associados à cultura da cana-de-açúcar / Effects of soybean proteases inhibitors on non-target organisms associated to sugarcane
Genes of plants expressing insect proteinase inhibitors have been introduced into plants for pest control. Proteases inhibitors are present in plant tissues, mainly in seeds, and act in response to predators and pathogens. The Bowman-Birk type and Kunitz type of serine proteases inhibitors (PI) from soybean seeds are been used to increase resistance of sugar
Publicado em: 2008