Sensory Spectrum
Mostrando 13-24 de 35 artigos, teses e dissertações.
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13. Removal of the transducer protein from sensory rhodopsin I exposes sites of proton release and uptake during the receptor photocycle.
The phototaxis receptor sensory rhodopsin-I (SR-I) was genetically truncated in the COOH terminus which leads to overexpression in Halobacterium salinarium and was expressed in the presence and absence of its transducer, HtrI. Pyranine (8-hydroxyl-1,3,6-pyrene-trisulfonate) was used as a pH probe to show that proton release to the bulk phase results from the
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14. Close correspondence between the action spectra for the blue light responses of the guard cell and coleoptile chloroplasts, and the spectra for blue light-dependent stomatal opening and coleoptile phototropism.
Fluorescence spectroscopy was used to characterize blue light responses from chloroplasts of adaxial guard cells from Pima cotton (Gossypium barbadense) and coleoptile tips from corn (Zea mays). The chloroplast response to blue light was quantified by measurements of the blue light-induced enhancement of a red light-stimulated quenching of chlorophyll a fluo
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15. Primary reactions of sensory rhodopsins
The first steps in the photocycles of the archaeal photoreceptor proteins sensory rhodopsin (SR) I and II from Halobacterium salinarum and SRII from Natronobacterium pharaonis have been studied by ultrafast pump/probe spectroscopy and steady-state fluorescence spectroscopy. The data for both species of the blue-light receptor SRII suggests that their p
The National Academy of Sciences.
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16. Excitatory amino acid receptors and synaptic transmission in the rat ventrobasal thalamus.
1. Extracellular single-neurone recordings were made in the ventrobasal thalamus (v.b.t.) of urethane-anaesthetized rats with multi-barrel ionophoretic electrodes in order to test the hypothesis that excitatory amino acid receptors are involved in the responses of these neurones to stimulation of sensory afferents. 2. Responses of neurones to either physiolo
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17. Future affective technology for autism and emotion communication
People on the autism spectrum often experience states of emotional or cognitive overload that pose challenges to their interests in learning and communicating. Measurements taken from home and school environments show that extreme overload experienced internally, measured as autonomic nervous system (ANS) activation, may not be visible externally: a person c
The Royal Society.
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18. Synthesis of a gene for sensory rhodopsin I and its functional expression in Halobacterium halobium.
We have designed, synthesized, and expressed in Halobacterium halobium a gene encoding sensory rhodopsin I (SR-I). The gene has been optimized for cassette mutagenesis by incorporating 30 unique restriction sites with uniform spacing throughout the 720-bp coding region. For expression, the coding region was placed downstream of the promoter and translation i
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19. Action Spectrum for Interaction between Visible and Far-Red Light on Face Chloroplast Orientation in Mougeotia1
The orientation of chloroplasts from profile to face position in Mougeotia can be controlled in two ways: by a typical phytochrome-mediated system or by continuous, simultaneous irradiation with far-red and visible light. In experiments with dichromatic irradiation of Mougeotia, the light conditions applied prevented the formation of a far-red-absorbing form
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20. Noise shaping in populations of coupled model neurons
Biological information-processing systems, such as populations of sensory and motor neurons, may use correlations between the firings of individual elements to obtain lower noise levels and a systemwide performance improvement in the dynamic range or the signal-to-noise ratio. Here, we implement such correlations in networks of coupled integrate-and-fire neu
The National Academy of Sciences.
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21. An ionophoretic study of the responses of rat caudal trigeminal nucleus neurones to non-noxious mechanical sensory stimuli.
1. Extracellular recordings of the responses of single caudal trigeminal nucleus neurones to non-noxious and noxious facial stimuli and to ionophoretically applied L-glutamate, L-aspartate and acetylcholine were made in urethane anaesthetized rats. 2. Neurones excited by non-noxious mechanical stimuli were located primarily in the magnocellular part of nucle
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22. Removal of transducer HtrI allows electrogenic proton translocation by sensory rhodopsin I.
Sensory rhodopsin I (sR-I) is a phototaxis receptor in halobacteria, which is closely related to the light-driven proton pump bacteriorhodopsin and the chloride pump halorhodopsin found in the same organisms. The three pigments undergo similar cyclic photoreactions, in spite of their different functions. In intact cells or isolated membranes sR-I is complexe
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23. Identification of a third rhodopsin-like pigment in phototactic Halobacterium halobium.
Mutant Halobacterium halobium strains deficient in all previously reported rhodopsin-like pigments show phototaxis responses comparable to those of wild-type strains. Spectroscopic analysis reveals the presence of a third retinal-containing pigment in the cells and their membrane fractions. It undergoes a photoreaction cycle with a half-time of approximately
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24. The eubacterium Ectothiorhodospira halophila is negatively phototactic, with a wavelength dependence that fits the absorption spectrum of the photoactive yellow protein.
The motile, alkalophilic, and extremely halophilic purple sulfur bacterium Ectothiorhodospira halophila is positively photophobotactic. This response results in the accumulation of bacteria in light spots (E. Hustede, M. Liebergesell, and H. G. Schlegel, Photochem. Photobiol. 50:809-815, 1989; D. E. McRee, J. A. Tainer, T. E. Meyer, J. Van Beeumen, M. A. Cus