Semiquantitative Pcr
Mostrando 13-24 de 193 artigos, teses e dissertações.
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13. Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semi-quantitative analysis
A sulfated fucan from Laminaria abyssalis marine alga prevented the interaction of HTLV-1 particles, purified from the MT-2 cell line, with HeLa cells. The infection obtained using a concentrated virus suspension was detected only by amplification of the newly synthesized HTLV-1 proviral cDNA by the nested-polymerase chain reaction (PCR). The sulfated polysa
Revista Brasileira de Farmacognosia. Publicado em: 01/04/2011
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14. The effect of down-regulation of Smad3 by RNAi on hepatic stellate cells and a carbon tetrachloride-induced rat model of hepatic fibrosis
Searching for effective Smad3 gene-based gene therapies for hepatic fibrosis, we constructed siRNA expression plasmids targeting the rat Smad3 gene and then delivered these plasmids into hepatic stellate cells (HSCs). The effect of siRNAs on the mRNA levels of Smad2, Smad3, Smad4, and collagens I-α1, III-α1 and IV-α1 (Colα1, Col3α1, Col4α1, respectivel
Brazilian Journal of Medical and Biological Research. Publicado em: 2011-02
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15. Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny.
Abstract ? The objective of this work was to distinguish the parental source of alleles in heterozygous progeny using semiquantitative polymerase chain reaction (PCR) in maize endosperm. Endosperms derived from direct and reciprocal single-cross hybrids between maize inbred lines L3 and L1113-01 were genotyped by semiquantitative PCR methodology (SQ-PCR) usi
Pesquisa Agropecuária Brasileira. Publicado em: 2011
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16. Análise da expressao gênica do vegf (vascular Endothelial growth factor) em placentas bovinas de animais clonados, produzidos in vitro e in vivo, Pelo método de rt-pcr semiquantitativo / ANALYSIS OF VEGF GENE EXPRESSION IN PLACENTAS OF CLONED BOVINES, PRODUCED IN VITRO AND IN VIVO, BY SEMIQUANTITATIVE RT-PCR)
O sucesso da produção de embriões por fecundação in vitro ou transferência nuclear depende de uma variedade de fatores, dos quais a formação de uma incompleta vascularização placentária representa uma das principais causas de perda gestacional precoce. Neste trabalho, avaliou-se a expressão relativa de Fator de Crescimento Endotélio-vascular (VE
ARS VETERINARIA. Publicado em: 2011
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17. Characterization of the dry bean polygalacturonase-inhibiting protein (PGIP) gene family during Sclerotinia sclerotiorum (Sclerotiniaceae) infection.
Polygalacturonase-inhibiting proteins are leucine-rich repeat proteins that inhibit fungal endopolygalacturonases. The interaction of polygalacturonase-inhibiting protein with endopolygalacturonases limits the destructive potential of endopolygalacturonases and may trigger plant defense responses induced by oligogalacturonides. We examined the expression of
Genetics and Molecular Research. Publicado em: 2011
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18. cDNA-AFLP analysis of gene expression differences between the flower bud and sprout-shoot apical meristem of Angelica sinensis (Oliv.) Diels
Angelica sinensis (Oliv.) Diels (Umbelliferae) is a well-known medicinal plant mainly distributed in Gansu Province of China. Its local and global demand is significant because of its food and medicinal applications. However, the early bolting rate of Angelica sinensis (Oliv.) Diels reaches 20%-60%, which seriously affects its food and medicinal qualities. T
Genetics and Molecular Biology. Publicado em: 2011
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19. Serum and Urinary C-Reactive Protein Concentrations in Dogs with Leptospirosis
Background: Leptospirosis is a bacterial disease caused by pathogenic strains of Leptospira, which may affect human beings and a wide range of both domestic and wild animals. The disease in dogs is still a challenge for clinicians, since definitive diagnosis may be reached only few days after overt clinical signs. Besides that, dogs with leptospiruria have z
Publicado em: 2011
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20. Transcriptional analysis of the human PAX9 promoter
OBJECTIVES: PAX9 belongs to the Pax family of transcriptional factor genes. This gene is expressed in embryonic tissues such as somites, pharyngeal pouch endoderm, distal limb buds and neural crest-derived mesenchyme. Polymorphisms in the upstream promoter region of the human PAX9 have been associated with human non-syndromic tooth agenesis. In the present s
Journal of Applied Oral Science. Publicado em: 2010-10
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21. Isolation and characterization of genes functionally involved in ovarian development of the giant tiger shrimp Penaeus monodon by suppression subtractive hybridization (SSH)
Suppression subtractive hybridization (SSH) libraries between cDNA in stages I (previtellogenic) and III (cortical rod) ovaries of the giant tiger shrimp (Penaeus monodon) were established. In all, 452 ESTs were unidirectionally sequenced. Sequence assembly generated 28 contigs and 201 singletons, 109 of which (48.0%) corresponding to known sequences previou
Genetics and Molecular Biology. Publicado em: 2010
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22. Clinical and laboratory status of patients with chronic Chagas disease living in a vector-controlled area in Minas Gerais, Brazil, before and nine years after aetiological treatment
Twenty-eight Chagas disease patients (CD), 22 with the indeterminate clinical form (IND) and six with the cardiac or digestive form (CARD/DIG), were treated with benznidazole and underwent clinical and laboratorial analysis before (IND and CARD/DIG) and nine years after [patients after treatment (CDt), patients with the indeterminate clinical form at treatme
Memórias do Instituto Oswaldo Cruz. Publicado em: 2009-12
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23. The performance of semi-quantitative differential PCR is similar to that of real-time PCR for the detection of the MYCN gene in neuroblastomas
Amplification of the MYCN gene in neuroblastomas is a potent biological marker of highly aggressive tumors, which are invariably fatal unless sound clinical management is applied. To determine the usefulness of semi-quantitative differential PCR (SQ-PCR) for accurate quantification of MYCN gene copy number, we evaluated the analytical performance of this met
Brazilian Journal of Medical and Biological Research. Publicado em: 2009-09
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24. Expression of Msx1 and Dlx1 during Dumbo rat head development: correlation with morphological features
The Dumbo rat possesses some characteristics that evoke several human syndromes, such as Treacher-Collins: shortness of the maxillary, zygomatic and mandibular bones, and low position of the ears. Knowing that many homeobox genes are candidates in craniofacial development, we investigated the involvement of the Msx1 and Dlx1 genes in the Dumbo phenotype with
Genetics and Molecular Biology. Publicado em: 01/05/2009