Saint Louis Encephalitis
Mostrando 13-23 de 23 artigos, teses e dissertações.
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13. Ecologia de Culex quinquefasciatus e de Culex nigripalpus no Parque Ecológico do Tietê, São Paulo, Brasil / Ecology of Culex quinquefasciatus and Culex nigripalpus at the Parque Ecológico do Tietê, São Paulo, Brasil.
Introduction - Culex quinquefasciatus has high synanthropy, infest human dwellings and is vector of nematoids and arbovirus from endemic areas, respectively, in Brazilian coast and in Central or North America. Culex nigripalpus has average synanthropy and can disperse through the anthropic environment carrying Saint Louis Virus and Equine Encephalitis Virus,
Publicado em: 2007
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14. Brazilian Flavivirus phylogeny based on NS5
In this work, a comprehensive phylogenetic study based on 600 base pair nucleotide and on putative 200 amino acid sequences of NS5 was carried out in order to establish genetic relationships among 15 strains of 10 Brazilian flaviviruses: Bussuquara, Cacipacore, dengue type 1, 2 and 4, Iguape, Ilheus, Rocio, Saint Louis encephalitis (SLE), and yellow fever. P
Memórias do Instituto Oswaldo Cruz. Publicado em: 2003-04
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15. Group B Arbovirus Structural and Nonstructural Antigens I. Serological Identification of Saint Louis Encephalitis Virus Soluble Antigens
Saint Louis encephalitis virus-infected cells were solubilized with Brij-58 nonionic detergent, and the clarified supernatant fluid was analyzed by diethyl-aminoethyl-cellulose chromatography. Three serologically distinguishable antigens were identified in viral protein peaks which eluted from diethylaminoethyl-cellulose at 0.05, 0.075, 0.125 and 0.2 m KCl.
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16. Group B Arbovirus Structural and Nonstructural Antigens II. Purification of Saint Louis Encephalitis Virus Intracellular Antigens
Three serologically distinct antigens were identified in homogenates of Saint Louis encephalitis (SLE) virus-infected cells after Brij-58 solubilization and diethylaminoethyl (DEAE)-cellulose chromatography. These antigens were designated as antigen I, II, and III. Immunodiffusion analyses showed that all antigen peaks which eluted from the DEAE-cellulose co
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17. Saint Louis Encephalitis Viral Ribonucleic Acid Replication Complex
Pulse-labeled Saint Louis encephalitis viral ribonucleic acid (RNA) is found in the cytoplasm of infected cells associated with a membranous structure which sediments with an average value of 250S. The integrity of the complex is destroyed by detergents and ribonuclease; however, it is stable in ethylenediaminetetraacetic acid (EDTA) which differentiates thi
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18. Structural and Nonstructural Proteins of Saint Louis Encephalitis Virus 1
Analysis of purified Saint Louis encephalitis (SLE) virus by acrylamide gel electrophoresis revealed that the virions contained three structural proteins designated SP-1, SP-2, and SP-3 which had molecular weights of 63,000, 18,000, and 8,500, respectively. The envelope contained proteins SP-1 and SP-3 which were removed from the nucleocapsid by nonionic det
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19. Synthesis of Saint Louis Encephalitis Virus Ribonucleic Acid in BHK-21/13 Cells
Infection of baby hamster kidney cells (BHK-21/13) with Saint Louis encephalitis (SLE) virus depressed the rate of protein and ribonucleic acid (RNA) synthesis until viral RNA synthesis began 6 hr postinfection (PI). Virus-directed RNA synthesis was subsequently inhibited until 12 hr PI when virion maturation began. The rate of protein synthesis reached a pe
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20. Identification of Saint Louis encephalitis virus mRNA.
Saint Louis encephalitis (SLE) virus-specific RNA was recovered from infected HeLa cells by sodium dodecyl sulfate (SDS)-phenol-chloroform extraction, and the molecular species were resolved by SDS-sucrose gradient centrifugation and agarose gel electrophoresis. Sucrose gradient centrifugation revealed the presence of a 45S species, minor 20 to 30S heterogen
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21. Group B Arbovirus Structural and Nonstructural Antigens III. Serological Specificity of Solubilized Intracellular Viral Proteins
Solubilized nonstructural antigen III from Saint Louis encephalitis (SLE)-Japanese B encephalitis (JBE)-West Nile (WN) and dengue-2 arbovirus-infected pig kidney cells was purified by employing Brij-58 solubilization, organic solvent extraction, and column chromatography. Diethylaminoethyl-cellulose column peak C eluate contained only intracellular viral env
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22. Solid-phase radioimmunoassay for antibodies to flavivirus structural and nonstructural proteins.
A micro-solid-phase radioimmunoassay (SPRIA) is described for quantitation of antibodies to purified flaviviruses as well as to the purified envelope glycoprotein and 80,000-molecular-weight viral nonstructural protein. Sera from mice experimentally infected with Saint Louis encephalitis (SLE) virus or from humans after a primary SLE virus infection reacted
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23. Epitope-Blocking Enzyme-Linked Immunosorbent Assays for the Detection of Serum Antibodies to West Nile Virus in Multiple Avian Species
We report the development of epitope-blocking enzyme-linked immunosorbent assays (ELISAs) for the rapid detection of serum antibodies to West Nile virus (WNV) in taxonomically diverse North American avian species. A panel of flavivirus-specific monoclonal antibodies (MAbs) was tested in blocking assays with serum samples from WNV-infected chickens and crows.
American Society for Microbiology.