Mostrando 1-12 de 73 artigos, teses e dissertações.
1. In vitro study of postbiotics from Lactobacillus plantarum RG14 on rumen fermentation and microbial population
ABSTRACT An in vitro study was carried out to identify the effects of different inclusion levels of postbiotics from Lactobacillus plantarum RG14 on rumen fermentation profiles, gas production kinetics, and microbial population in rumen fluid collected from goats. Postbiotics were added at different levels (0, 0.3, 0.6, 0.9, and 1.2%) and incubated for 72 h
R. Bras. Zootec.. Publicado em: 08/11/2018
2. Polyethylene glycol and polyvinylpirrolidone effect on bacterial rRNA extraction and hybridization from cells exposed to tannins.
In order to detect fluctuations in ruminal microbial populations due to forage tannins using 16S ribosomal RNA (rRNA) probes, recovery of intact rRNA is required. The objective of this work was to evaluate the effect of polyethylene glycol (PEG) and polyvinylpirrolidone (PVP) on extraction of bacterial rRNA, in the presence of tannins from tropical legume fo
Pesquisa Agropecuária Brasileira. Publicado em: 2011
3. Efeito de polietilenoglicol e polivinilpirrolidona na extração e hibridização de rRNA bacteriano de células expostas a taninos
A recuperação de RNA ribossômico (rRNA) intacto é necessária para a detecção de flutuações na população microbiana ruminal decorrentes dos taninos de forrageiras, utilizando-se sondas para 16S rRNA. O objetivo deste trabalho foi avaliar o efeito de polietilenoglicol (PEG) e polivinilpirrolidona (PVP) na extração de rRNA bacteriano, em presença
Pesquisa Agropecuária Brasileira. Publicado em: 2003-09
4. Albusin B, a Bacteriocin from the Ruminal Bacterium Ruminococcus albus 7 That Inhibits Growth of Ruminococcus flavefaciens
An ∼32-kDa protein (albusin B) that inhibited growth of Ruminococcus flavefaciens FD-1 was isolated from culture supernatants of Ruminococcus albus 7. Traditional cloning and gene-walking PCR techniques revealed an open reading frame (albB) encoding a protein with a predicted molecular mass of 32,168 Da. A BLAST search revealed two homologs of AlbB from th
American Society for Microbiology.
5. Purification and Properties of Cellobiosidase from Ruminococcus albus
An enzyme active against p-nitrophenyl-β-d-cellobioside was purified from Ruminococcus albus to homogeneity. The enzyme was identified as a cellobiosidase.
6. The use of 16S rRNA-targeted oligonucleotide probes to study competition between ruminal fibrolytic bacteria: development of probes for Ruminococcus species and evidence for bacteriocin production.
A total of six oligonucleotide probes, complementary to the 16S rRNA, were evaluated for quantitative and determinative studies of Ruminococcus albus and Ruminococcus flavefaciens. On the basis of specificity studies, probes for R. albus (probe RAL196) and R. flavefaciens (probe RFL196) were selected to quantitate these species in mixed culture. In combinati
7. Muralytic Activities of Ruminococcus albus 8
Ruminococcus albus 8 was cultured with isolated alfalfa cell walls as the carbon source. The culture broth was assayed for muralytic enzyme activities. The effect, with respect to the production of such muralytic enzymes, of growing the microorganism on different carbon sources was also investigated. Also, the rates of solubilization and utilization by R. al
8. Phenylpropanoic Acid: Growth Factor for Ruminococcus albus
Phenylpropanoic acid accounted for part of the stimulatory effect of rumen fluid on the rate of growth and of cellulose digestion by cultures of Ruminococcus albus strain 8 grown on a chemically defined medium. As little as 3 μM concentration gave maximum response.
9. Enumeration of transconjugated Ruminococcus albus and its survival in the goat rumen microcosm.
A transconjugant Ruminococcus albus A3 culture was released into a goat rumen, and the extent of its survival in the rumen microcosm was measured by distinguishing this bacterium from indigenous microbes by antibiotic resistance. A3 cells remained roughly constant for 14 days in this goat rumen.
10. POLYSACCHARIDE STORAGE AND GROWTH EFFICIENCY IN RUMINOCOCCUS ALBUS
Hungate, R. E. (University of California, Davis). Polysaccharide storage and growth efficiency in Ruminococcus albus. J. Bacteriol. 86:848–854. 1963.—Ruminococcus albus strain RAM requires biotin, p-aminobenzoic acid, pyridoxamine, isovalerate, isobutyrate, 2-methylbutyrate, and either cysteine or sulfide. Rumen fluid and casein hydrolysate improve growt
11. Isolation and properties of beta-glucosidase from Ruminococcus albus.
An enzyme active against p-nitrophenyl-beta-D-glucoside was purified from logarithmic-phase cells of Ruminococcus albus cultivated in a medium containing ball-milled cellulose. The purification yielded homogeneous enzyme after an approximately 520-fold increase in specific activity and a 9% yield. The enzyme was identified as a beta-glucosidase because it ca
12. Phenylacetic and Phenylpropionic Acids Do Not Affect Xylan Degradation by Ruminococcus albus
Since the addition of either ruminal fluid or a combination of phenylacetic and phenylpropionic acids (PAA/PPA) has previously been shown to dramatically improve cellulose degradation and growth of Ruminococcus albus, it was of interest to determine the effects of these additives on xylan-grown cultures. Although cell-bound xylanase activity increased when e
American Society for Microbiology.