Ruled Surface
Mostrando 13-24 de 31 artigos, teses e dissertações.
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13. Differentiation of Serratia marcescens 274 into swimmer and swarmer cells.
We describe a new sensory response in the enteric bacterium Serratia marcescens. When grown in liquid media, the bacteria were short rods with one to two flagella and displayed classical swimming behavior. Upon transfer to a solid surface (0.7 to 0.8T% agar medium), the bacteria underwent a dramatic change of form. They ceased septation, elongated, and expre
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14. Surface antigen detected by a Schistosoma mansoni monoclonal antibody in worm extracts and kidney deposits of infected mice and hamsters.
Four monoclonal antibodies (MAbs) derived from a Schistosoma mansoni-infected mouse reacted against the tegument or the cell layer of the digestive tract of the adult worm. They also showed similar patterns of immunofluorescence staining when schistosomula were used as antigens. Two of the MAbs (4A10 and 4D3) recognized immune complexes deposited in the kidn
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15. Effects of different transferrin forms on transferrin receptor expression, iron uptake, and cellular proliferation of human leukemic HL60 cells. Mechanisms responsible for the specific cytotoxicity of transferrin-gallium.
We have previously shown that human leukemic cells proliferate normally in serum-free media containing various transferrin forms, but the addition of transferrin-gallium leads to inhibition of cellular proliferation. Because gallium has therapeutic potential, the effects of transferrin-gallium on leukemic cell proliferation, transferrin receptor expression,
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16. Hafnia alvei, a probable cause of diarrhea in humans.
Hafnia alvei, a member of the family Enterobacteriaceae, was the only species of bacteria cultured from the stool of a 9-month-old child who was admitted with a 3-day history of watery diarrhea. The isolated strain of H. alvei failed to produce heat-labile or heat-stable enterotoxins or Shiga-like toxin I or II and did not invade HeLa cells, nor did it cause
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17. Axial rotation of sliding actin filaments revealed by single-fluorophore imaging
In the actomyosin motor, myosin slides along an actin filament that has a helical structure with a pitch of ≈72 nm. Whether myosin precisely follows this helical track is an unanswered question bearing directly on the motor mechanism. Here, axial rotation of actin filaments sliding over myosin molecules fixed on a glass surface was visualized through fluor
The National Academy of Sciences of the USA.
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18. Molecular Characterization of the Mycoplasma gallisepticum pvpA Gene Which Encodes a Putative Variable Cytadhesin Protein
A putative cytadhesin-related protein (PvpA) undergoing variation in its expression was identified in the avian pathogen Mycoplasma gallisepticum. The pvpA gene was cloned, expressed in Escherichia coli, and sequenced. It exhibits 54 and 52% homology with the P30 and P32 cytadhesin proteins of the human pathogens Mycoplasma pneumoniae and Mycoplasma genitali
American Society for Microbiology.
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19. The membrane (M1) protein of influenza virus occurs in two forms and is a phosphoprotein.
The membrane (M1) protein of influenza virus was found to be heterogenous and to occur in two forms in the virus particle. The two forms of M1 were found in virus which was produced both early and late after infection and in infected cells. The two forms could be separated on polyacrylamide gels under specific conditions. The two components of M1 contained s
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20. Modulation of the Activity of an Avian Gene Transferred into a Mammalian Cell by Cell Fusion
Mouse A9 cells, deficient in hypoxanthine phosphoribosyltransferase (EC 2.4.2.8), were fused with normal chick erythrocytes and selected in hypoxanthine-aminopterin-thymidine medium for cells with hypoxanthine phosphoribosyltransferase activity. Recovered hybrid cells produced the chick hypoxanthine phosphoribosyltransferase exclusively, as demonstrated by e
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21. Conjugal Transfer of Lactose-Fermenting Ability Among Streptococcus cremoris and Streptococcus lactis Strains †
Streptococcus cremoris C3 was found to transfer lactose-fermenting ability to LM2301, a Streptococcus lactis C2 lactose-negative streptomycin-resistant (Lac− Strr) derivative which is devoid of plasmid deoxyribonucleic acid (DNA); to LM3302, a Lac− erythromycin-resistant (Eryr) derivative of S. lactis ML3; and to BC102, an S. cremoris B1 Lac− Eryr deri
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22. Conjugal Transfer of Genetic Information in Group N Streptococci †
Streptococcus lactis strains ML3 and C2O and S. lactis subsp. diacetylactis strains DRC3, 11007, and WM4 were found to transfer lactose-fermenting ability to LM0230, an S. lactis C2 lactose-negative (Lac−) derivative which is devoid of plasmid deoxyribonucleic acid (DNA). Lactose-positive streptomycin-resistant (Lac+ Strr) recombinants were found when the
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23. Effects of divalent cations on muscarinic receptor cationic current in smooth muscle from guinea-pig small intestine.
1. Effects of Mg2+ and Ca2+ on muscarinic receptor cationic current (Icat) in guinea-pig ileal smooth muscle cells have been studied using patch-clamp techniques (whole-cell recording). Icat was activated either by externally applied carbachol or, to bypass receptors, by intracellular GTP-gamma-S. 2. Independently of the main permeant cation the current-volt
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24. Extrasynaptic Release of GABA by Retinal Dopaminergic Neurons
GABA release by dopaminergic amacrine (DA) cells of the mouse retina was detected by measuring Cl− currents generated by isolated perikarya in response to their own neurotransmitter. The possibility that the Cl− currents were caused by GABA release from synaptic endings that had survived the dissociation of the retina was ruled out by examining confocal
American Physiological Society.