Rooster
Mostrando 13-24 de 42 artigos, teses e dissertações.
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13. Image cytometric measurement of nuclear DNA content in pepper (Capsicum annuum L.) / Determinação do conteúdo de DNA em pimentão (Capsicum annuum L.) por citometria de imagem
Capsicum genus is one of the most important members of the Solanaceae family with high economic value. Out of 25 known species, five are extensively cultivated. Capsicum annuum is the most important commercially by its agronomic value as spice. In despite of this importance, the nuclear DNA content of C. annuum is still uncertain with values from the literat
Publicado em: 2006
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14. Breeding performance of Lohmann- LSL roosters fed diets supplemented with different oils and levels of vitamin E / Desempenho reprodutivo de galos Lohman-LSL alimentados com raÃÃes suplementadas com diferentes fontes de Ãleo e nÃveis de vitamina E
The aim this study was evaluate breeding and productive performance of Lohmann- LSL roosters. 112 breeding roosters from 16 to 32 weeks of age were allotted to a randomized block design in a 3x2 + 1factorial arrangement, with three sources of oil (soybean, canola and sunflower in 3%) and two levels of antioxidant (200 and 400 mg of vitamin E/kg of diet) and
Publicado em: 2004
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15. Induction of vitellogenin synthesis by estrogen in avian liver: relationship between level of vitellogenin mRNA and vitellogenin synthesis.
We have investigated the estrogen-mediated induction of vitellogenin synthesis in rooster liver. We compared the concentrations of vitellogenin messenger RNA (mRNA) in the liver with the concentrations of vitellogenin in the sera of roosters that had recieved various treatments with estrogen. We found no vitellogenin mRNA in the livers of the unstimulated ro
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16. Phosphorylation/dephosphorylation of the repressor MDBP-2-H1 selectively affects the level of transcription from a methylated promoter in vitro.
We have previously shown that in vivo estradiol-dependent dephosphorylation of MDBP-2-H1 (a member of the histone H1 family) correlates with the loss of in vitro preferential binding to methylated DNA. To study the effects of the phosphorylation/dephosphorylation of MDBP-2-H1 on the expression of the avian vitellogenin II gene, we optimised an in vitro trans
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17. DIRECT AND INDIRECT FLUORESCENT-ANTIBODY TECHNIQUES FOR THE PSITTACOSIS-LYMPHOGRANULOMA VENEREUM-TRACHOMA GROUP OF AGENTS1
Ross, Martin R. (Connecticut State Department of Health, Hartford) and Earle K. Borman. Direct and indirect fluorescent-antibody techniques for the psittacosis-lymphogranuloma venereum-trachoma group of agents. J. Bacteriol. 85:851–858. 1963.—Direct and indirect fluorescent-antibody (FA) techniques were developed for the detection of group antigen in inf
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18. Effect of estrogen on gene expression: purification of vitellogenin messenger RNA.
We report initial studies on estrogen-mediated regulation of egg yolk protein synthesis in the rooster. Egg yolk proteins are normally synthesized as a large precursor, vitellogenin, in the liver of the laying hen; roosters synthesize vitellogenin only when treated with estrogen. Polysomal RNA from the liver of estrogen-treated roosters was translated in a r
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19. Histone H4 hyperacetylation and rapid turnover of its acetyl groups in transcriptionally inactive rooster testis spermatids.
In order to study the relationship between acetylation of histones, chromatin structure and gene activity, the distribution and turnover of acetyl groups among nucleosomal core histones and the extent of histone H4 acetylation were examined in rooster testis cell nuclei at different stages of spermatogenesis. Histone H4 was the predominant acetylated histone
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20. The methylated DNA binding protein-2-H1 (MDBP-2-H1) consists of histone H1 subtypes which are truncated at the C-terminus.
The methylated DNA binding protein-2-H1 (MDBP-2-H1), present in rooster liver, is a member of the histone H1 family which inhibits transcription by binding selectively to methylated promoters. Here we have determined the primary structure of MDBP-2-H1. A comparison between histone H1 and MDBP-2-H1 was achieved by analyzing reversed phase HPLC-purified and V8
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21. Comparisons of Venezuelan encephalitis virus strains by hemagglutination-inhibition tests with chicken antibodies.
Twenty strains of Venezuelan encephalitis (VE) virus inoculated intravenously in large doses into roosters produced hemagglutination-inhibition (HI) antibodies detectable in plasmas within 7 to 10 days. No signs of illness occurred, and there was no evidence of viral growth in tissues since blood concentrations of infectious virus steadily decreased after in
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22. An avian 40 KDa nucleoprotein binds preferentially to a promoter sequence containing one single pair of methylated CpG.
In vitro transcription competition with oligonucleotides has shown that a down regulating factor can be displaced by a methylated oligonucleotide covering a specific region of the avian vitellogenin II gene promoter (Proc. Natl. Acad. Sci USA, (1990) 87, 3047-3051). Gel mobility shift and competition assays show that a protein binding preferentially to methy
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23. Serotyping of Chlamydia: antibodies to lymphogranuloma venereum strains compared by microimmunofluorescence and neutralization tests.
The microimmunofluorescence test with hyperimmune rooster sera against lymphogranuloma venereum strains gave the same serotype patterns obtained in the standard mouse serum system. Results in a plaque reduction test appeared similar when only 3 prototype strains were tested, but differed when a battery of 11 strains were tested.
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24. A Specific Hepatic Transfer RNA for Phosphoserine*
Radioactive O-phosphoryl-L-serine was detected after alkaline deacylation of rat and rooster liver [3H]seryl-tRNA acylated in vitro with homologous synthetases. Ribonuclease treatment of this tRNA yielded a compound with the properties of phosphoseryl-adenosine. Benzoylated DEAE-cellulose chromatography of seryl-tRNA yielded four distinct peaks, only one of