Rna Viral Diagnostic Use
Mostrando 1-12 de 14 artigos, teses e dissertações.
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1. Vírus Dengue Sorotipo 3 (DENV-3) no Brasil: Estudos sobre Patogenia, Sítios de Replicação, Filogenia e Evolução Molecular / Dengue virus serotype 3 (DENV-3) in Brazil: Studies on Pathogenesis, Replication Sites, Phylogeny and Molecular Evolution
Dengue é uma importante arbovirose (arthropod-borne virus) e constitui um grave problema de saúde pública não só no Brasil, mas também nos países de clima tropical. O Aedes aegypti é o principal vetor dos vírus dengue (DENV) e está presente na maioria dos países entre as latitudes 35N e 35S. Neste trabalho, apresentamos quatro estudos. No primeiro
Publicado em: 2009
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2. Determinação do RNA-VHC no sêmen de pacientes cronicamente infectados pelo vírus da Hepatite C / Determinations of the RNA-HCV in semen from chronically patients infected by the hepatitis C vírus
Introduction: Hepatitis C vírus is a huge problem for public health, and its global prevalence is estimated around 3%. Its transmission by seminal fluid is still in discussion in several fields, such as assisted reproduction and in studies about risk factors, whether the hepatitis C virus is an STD (sexually transmitted disease) or not. Twenty-three patient
Publicado em: 2009
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3. Molecular detention and characterization of astrovirus and norovirus in cases of acute gastroenteritis in children hospitalized in the city of Rio De Janeiro / Detecção e caracterização molecular de astrovírus e norovírus em casos de gastroenterite aguda em crianças hospitalizadas no município do Rio de Janeiro
Acute viral gastroenteritis is responsible for significant morbidity and mortality among children and human astrovirus (HAstV) and norovirus (NV) have been identified increasingly worldwide as agents of those infections. The goals of this study were to determine the prevalence and molecular characterization of HAstV and NV by reverse-transcription polymerase
Publicado em: 2006
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4. Infectious bronchitis virus: detection and vaccine Strain differentiation by semi-nested RT-PCR
A semi-nested reverse transcription-polymerase chain reaction (Semi-N-RT-PCR) was developed and used to detect the S glycoprotein gene of infectious bronchitis virus (IBV) strains and to discriminate H120 vaccine strain from other strains. Viral RNA was extracted from the allantoic fluid of chicken embryos and from tissues of chickens experimentally infected
Revista Brasileira de Ciência Avícola. Publicado em: 2005-03
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5. Caracterização de anticorpos monoclonais contra rotavírus bovino e suas aplicações como ferramenta de diagnóstico. / Characterization of monoclonal antibodies against bovine rotavirus and their diagnostic applications.
Rotavirus, a member of the Reoviridae family, is the most important etiologic agent of diarrheas in bovine. Complete particules measure 70 nm in diameter, are nonenveloped and have a distinctive double-layered icosahedral protein capsid that consists of an outer and an inner layer. Within the inner capsid is a third layer, the core, that contains the virus g
Publicado em: 2005
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6. Surveillance of Childhood Influenza Virus Infection: What Is the Best Diagnostic Method To Use for Archival Samples?
Despite the clinical importance of influenza virus in pediatric respiratory infections, the optimal set of diagnostic tests to use when conducting studies using archival samples is not clear. In this study, we compared diagnostic tests for influenza virus in 75 children younger than 5 years of age who presented with symptomatic respiratory infection during o
American Society for Microbiology.
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7. Expression of Sendai virus nucleocapsid protein in a baculovirus expression system and application to diagnostic assays for Sendai virus infection.
The most common diagnostic technique for the detection of Sendai virus infection in rodents is serological evaluation by enzyme-linked immunosorbent assay (ELISA) with semipurified preparations of whole virions as antigens. This assay often suffers from a lack of specificity. The goal of the present project was to develop more specific antigens for use in di
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8. Molecular Assays for Detection of Human Metapneumovirus
The recent description of the respiratory pathogen human metapneumovirus (hMPV) has highlighted a deficiency in current diagnostic techniques for viral agents associated with acute lower respiratory tract infections. We describe two novel approaches to the detection of viral RNA by use of reverse transcriptase PCR (RT-PCR). The PCR products were identified a
American Society for Microbiology.
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9. Molecular Methods for Diagnosis of Viral Encephalitis
Hundreds of viruses cause central nervous system (CNS) disease, including meningoencephalitis and postinfectious encephalomyelitis, in humans. The cerebrospinal fluid (CSF) is abnormal in >90% of cases; however, routine CSF studies only rarely lead to identification of a specific etiologic agent. Diagnosis of viral infections of the CNS has been revolutioniz
American Society for Microbiology.
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10. Viral Replication and Host Gene Expression in Alveolar Macrophages Infected with Ebola Virus (Zaire Strain)
In order to characterize the cellular response to and identify potential diagnostic markers for the early detection of Ebola virus, an in vitro culture system involving nonhuman primate alveolar macrophages was developed. Ebola virus replication in the alveolar macrophages was characterized by plaque assay, immunohistochemical analysis, and in situ hybridiza
American Society for Microbiology.
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11. Specificity of dot hybridization assay in the presence of rRNA for detection of rotaviruses in clinical specimens.
Nucleic acid hybridization is used to identify viral genomic sequences in clinical and environmental samples. However, RNA virus genomes have been reported to hybridize to mammalian rRNA from uninfected cells under stringent conditions, and caution has therefore been advised in the use of nucleic acid probes for detection of RNA viruses. To evaluate the effe
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12. Evaluation of a TaqMan PCR Assay To Detect Rabies Virus RNA: Influence of Sequence Variation and Application to Quantification of Viral Loads
Published assays that use TaqMan PCR are consistently sensitive, rapid, and readily transferable. Here we describe a TaqMan PCR-based method for the detection of rabies virus (RV) RNA in tissue samples. We show that the method has an acceptable linear range, is both sensitive and specific, and, importantly, correlates with the concentration of infectious vir
American Society for Microbiology.