Rinm5f Cells
Mostrando 1-12 de 28 artigos, teses e dissertações.
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1. Toxicidade de aminoacetona e células produtoras de insulina / Cytotoxity of aminoacetone on insulin-producing cells
Danos induzidos por hiperglicemia em tecidos no diabetes são caracterizados por quatro mecanismos conectados: aumento do fluxo metabólico através da via do poliol, ativação da proteína quinase C (PKC), aumento da atividade da via das hexosaminas e aumento da produção intracelular dos precursores dos produtos finais de glicação avançada (AGEs). Ent
Publicado em: 2010
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2. Alterations in Dexras1 expression mediated by STAT5 and GR cross-talk contribute to the modulation of insulin secretion during pregnancy and lactation. / Alterações na expressão de Dexras1 mediada pela cooperação entre STAT5 e GR contribuem para modulação da secreção de insulina na gestação e lactação.
It is not clear how glucocorticoid receptor (GR) counteracts STAT5 activity during the transition of pregnancy to lactation. Dexras1 is a small G protein activated by dexamethasone (DEX) that controls cell morphology, growth, etc. In the present study we detected Dexras1 expression in pancreatic beta cell. DEX induces Dexras1 expression in RINm5F cells, whic
Publicado em: 2010
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3. Produção e uso da proteína de fusão VP22.Pax4 na diferenciação de células-tronco em células produtoras de insulina / Production and use of the VP22.Pax4 fusion protein for stem cells differentiation into insulin-producing cells
O Diabetes Mellitus tipo I (DM1) é causado pela destruição auto-imune das células β pancreáticas, encontradas na porção endócrina do pâncreas, constituída pelas ilhotas pancreáticas. As células β são responsáveis pela produção e liberação de insulina, um hormônio que promove a internalização da glicose pelas células. Junto com
Publicado em: 2010
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4. Mecanismos moleculares do efeito citotoxico da dexametasona em linhagens de celulas beta e ilhotas pancreaticas / Molecular mechanisms of the cytotoxic effect of dexamethasone in insulin producing cells and pancreatic islets
Introdução/Objetivos. A produção de espécies reativas de oxigênio (EROs) faz parte de diversos processos fisiológicos. Nos últimos anos, o aumento de EROs têm sido associado ao desenvolvimento de diversas doenças, dentre elas o Diabetes Mellitus Tipo 2. As células beta pancreáticas são notadamente mais suscetíveis ao estresse oxidativo devido a
Publicado em: 2009
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5. Análise in vitro da atividade antioxidante do suco e extrato de maçã em células RINm5f submetidas a diferentes condições de estresse oxidativo
The phenolic compounds, found in vegetables and fruits, may have a high antioxidant activity. Apple, worlds second largest produced fruit, is classified as having one of the high antioxidant potential due to its content and profile of phenolic compounds. The aim of this research was to evaluate the antioxidant activity of apple and check its effect on cells
Publicado em: 2009
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6. Modulation of intracellular signaling pathways in insulin-producing cells lines by INGAP-PP / Analise das vias de sinalização intracelulares em linhagens de celulas produtoras de insulina expostas ao INGAP-PP
The pentadecapeptide comprising the 104-118 aminoacid sequence of the ilotropin-derived Reg3- related islet neogenesis associated protein (INGA-PP) has been implicated in pancreatic beta-cell neogenesis and enhancement of the insulin secretion in pancreatic islets. There is little information regarding the mechanism of action of the polypeptide. The aim of t
Publicado em: 2009
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7. Study of endoplasmatic reticulum stress-related proteins in the maternal pancreatic islets remodeling during the peripartum / Estudo da expressão das proteínas envolvidas no estresse de retículo endoplasmático durante o remodelamento das ilhotas pancreáticas maternas no período perinatal
During gestation occurs increase on the proliferation and apoptosis reduction of pancreatic b cells. Prolactin (PRL) promotes these changes which are reverted after delivery. Dexametasone (DEX) in vitro opposed to PRL. We evaluate whether endoplasmatic reticulum stress (ERS) was involved on post-delivery apoptosis and glycocorticoids (GC) participate on this
Publicado em: 2009
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8. Obtention of human pancreatic islets for transplantation through an increase in cell mass and an immunoisolation with biocompatible microcapsules / Ilhotas pancreáticas humanas viáveis para o transplante através do aumento da massa de células e do imunoisolamento com microcápsulas biocompatíveis
Islet transplantation has been proposed as a promising therapeutic strategy for the cure of type 1 diabetes mellitus (DM), however, its application to all diabetic patients is still not possible due to the limited source of islets or β cells and to the need of an immunosuppressive treatment of the recipient to avoid graft rejection. The use of immunosup
Publicado em: 2009
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9. Receptors couple to L-type calcium channels via distinct Go proteins in rat neuroendocrine cell lines.
1. The present study examines the hypothesis of G protein subtype selectivity in receptor-induced inhibition of calcium channel currents (ICa) in the insulin-secreting RINm5F and pituitary GH3 rat cell lines. Specificity of receptor coupling to G proteins was studied by infusion of purified G alpha isoforms into cells via a patch pipette. 2. In RINm5F cells,
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10. Galanin inhibits a dihydropyridine-sensitive Ca2+ current in the RINm5f cell line.
Mechanisms of action of the neuropeptide galanin, a putative neuromodulator in the central and peripheral nervous systems, have been evaluated extensively in insulin-secreting cells isolated from pancreas and cell lines derived from pancreatic tumors. Galanin inhibits insulin secretion from these cells through several mechanisms, including activation of ATP-
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11. Activators of protein kinase C depolarize insulin-secreting cells by closing K+ channels.
Carbohydrate stimuli of insulin secretion depolarize the pancreatic B cell and the B-cell line RINm5F by inhibiting ATP-sensitive K+ channels. We examined the possibility that this effect is mediated by activation of protein kinase C. In RINm5F cells, the triose D-glyceraldehyde evoked a rapid increase of the mass of 1,2-diacylglycerol, the endogenous activa
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12. Rapid and reversible secretion changes during uncoupling of rat insulin-producing cells.
To determine whether insulin secretion is affected by a blockage of gap junctions between B cells, we have studied the secretion of rat pancreatic islets of Langerhans, primary dispersed islet cells, and cells of the RINm5F line, during short-term exposure to heptanol. Within minutes, this alkanol blocked gap junctions between the B cells of intact islets an