Ribosomal Protein L10
Mostrando 1-12 de 169 artigos, teses e dissertações.
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1. Identification of reference genes for gene expression normalization in safflower (Carthamus tinctorius)
ABSTRACT Safflower (Carthamus tinctorius L., Asteraceae) is an important oil crop and medicinal plant. Gene expression analysis is gaining importance in the research of safflower. Quantitative PCR has become a powerful method for gene study. Reference genes are one of the major qualification requirements of qPCR because they can reduce the variability. To id
Rev. bras. farmacogn.. Publicado em: 2016-10
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2. Clonagem, expressão, purificação e caracterização estrutural da proteína ribossomal L10 humana recombinante / Cloning, periplasmic expression, purification and structural characterization of human ribosomal protein L10 recombinant
A proteína ribossomal L10 (RP L10) é uma forte candidata a ser incluída na classe de proteínas supressoras de tumor. Também denominada QM, a proteína em questão é conhecida por participar da ligação das subunidades ribossomais 60S e 40S e da tradução de mRNAs. Possui massa molecular entre 24 a 26 kDa e ponto isoelétrico (pI) 10,5. A seqüência
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 01/12/2009
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3. Modulação dos genes de relógio Per1, Cry1b, Clock e da melanopsina por endotelina-1 em células embrionárias de Danio rerio / Modulation of clock genes Per1, Cry1b, Clock and of melanopsin by endothelin-1 in Danio rerio embryonic cells
Biological clocks are endogenous timekeepers that are present both in eukaryotic as in prokaryotic organisms. Different clocks have different periods, and those that have about 24h of oscillation are called circadian clocks. In mammals, the first identified circadian clock is located in the suprachiasmatic nucleus, in the hipothalamus. It is now well known t
Publicado em: 2007
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4. Autogenous control of Escherichia coli ribosomal protein L10 synthesis in vitro.
The DNA-dependent in vitro synthesis of Escherichia coli ribosomal protein L10 was inhibited when L10 was added to the protein-synthesizing incubations. Addition of L10 had little or no effect on the synthesis of ribosomal protein L12, elongation factor Tu (tufB), or the beta and beta' subunits of RNA polymerase. In addition, ribosomal protein L12 did not in
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5. Studies on the RNA and protein binding sites of the E. coli ribosomal protein L10.
We have used modification of specific amino acid residues in the E. coli ribosomal protein L10 as a tool to study its interactions with another ribosomal protein, L7/L12, as well as with ribosomal core particles and with 23S RNA. The ribosome and RNA binding capability of L10 was found to be inhibited by modification of one more of its arginine residues. Thi
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6. Studies on the binding of the ribosomal protein complex L7/12-L10 and protein L11 to the 5'-one third of 23S RNA: a functional centre of the 50S subunit.
The RNA binding sites of the protein complex of L7/12 dimers and L10, and of protein L11, occur within the 5'-one third of 23S RNA. Binding of the L7/12-L10 protein complex to the 23S RNA is stimulated by protein L11 and vice-versa. This is the second example to be established of mutual stimulation of RNA binding by two ribosomal proteins or protein complexe
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7. Ribosomal protein L4 stimulates in vitro termination of transcription at a NusA-dependent terminator in the S10 operon leader.
The 11-gene S10 ribosomal protein operon of Escherichia coli is under the autogenous control of L4, the product of the third gene of the operon. Ribosomal protein L4 inhibits both transcription and translation of the operon. Our in vivo studies indicated that L4 regulates transcription by causing premature termination within the untranslated S10 operon leade
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8. Insertions of transposon Tn5 into ribosomal protein PNA polymerase operons.
The genetic organization and interrelationships between the two ribosomal protein transcription units (the L11 and L10 operons) from near 89 min on the Escherichia coli chromosome were studied by using insertional mutations generated by the kanamycin-resistant transposable element Tn5. The polar effects of Tn5 insertions on the expression of the L11, L1, L10
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9. Autogenous control is not sufficient to ensure steady-state growth rate-dependent regulation of the S10 ribosomal protein operon of Escherichia coli.
The regulation of the S10 ribosomal protein operon of Escherichia coli was studied by using a lambda prophage containing the beginning of the S10 operon (including the promoter, leader, and first one and one-half structural genes) fused to lacZ. The synthesis of the lacZ fusion protein encoded by the phage showed the expected inhibition during oversynthesis
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10. Translational control of ribosomal protein L10 synthesis occurs prior to formation of first peptide bond
A simplified DNA-directed in vitro system has been developed to study the regulation of the synthesis of ribosomal protein L10 by measuring the formation of the first dipeptide, fMet-Ala. The results show that the inhibition of L10 synthesis by L10 (autoregulation) occurs at or prior to the formation of the first peptide bond.
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11. Escherichia coli ribosomal protein L10 is rapidly degraded when synthesized in excess of ribosomal protein L7/L12.
In Escherichia coli the genes encoding ribosomal proteins L10 and L7/12, rplJ and rplL, respectively, are cotranscribed and subject to translational coupling. Synthesis of both proteins is coordinately regulated at the translational level by binding of L10 or a complex of L10 and L7/L12 to a single target in the mRNA leader region upstream of rplJ. Unexpecte
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12. Autogenous control of the S10 ribosomal protein operon of Escherichia coli: genetic dissection of transcriptional and posttranscriptional regulation.
The S10 ribosomal protein operon is regulated autogenously by the product of one of the genes of the operon, the gene encoding ribosomal protein L4. We have used site-directed mutagenesis to isolate leader mutations affecting L4 control. The phenotypes of these mutants demonstrate that L4 regulates both transcription and translation of the S10 operon. Severa