Rhodanese
Mostrando 1-12 de 42 artigos, teses e dissertações.
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1. Atividade e população microbiana envolvida nas transformações do enxofre em solos com diferentes vegetações.
O objetivo deste trabalho foi determinar as populações microbianas e suas atividades envolvidas no ciclo do S, de solos virgem e cultivado, e as suas relações com as diferentes frações de S no solo. Das populações de microrganismos pesquisados, apenas foram encontradas as bactérias autótrofas oxidantes de S0 em pH 5,0 e as heterótrofas oxidantes d
Pesquisa Agropecuária Brasileira. Publicado em: 2011
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2. Rhodanese isozymes in three subjects with Leber's optic neuropathy.
Previous studies have reported an association between Leber's optic neuropathy and deficiency of rhodanese activity in liver and rectal mucosa. We have studied the rhodanese isozymes in liver biopsies from three subjects with Leber's optic neuropathy. The rhodanese isozyme patterns were indistinguishable from controls both in relative intensity and position
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3. Formation of a selenium-substituted rhodanese by reaction with selenite and glutathione: Possible role of a protein perselenide in a selenium delivery system
Selenophosphate is the active selenium-donor compound required by bacteria and mammals for the specific synthesis of Secys-tRNA, the precursor of selenocysteine in selenoenzymes. Although free selenide can be used in vitro for the synthesis of selenophosphate, the actual physiological selenium substrate has not been identified. Rhodanese (EC 2.3.1.1) no
National Academy of Sciences.
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4. The rhodanese/Cdc25 phosphatase superfamily: Sequence–structure–function relations
Rhodanese domains are ubiquitous structural modules occurring in the three major evolutionary phyla. They are found as tandem repeats, with the C-terminal domain hosting the properly structured active-site Cys residue, as single domain proteins or in combination with distinct protein domains. An increasing number of reports indicate that rhodanese modules ar
Oxford University Press.
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5. Improved Assay for Rhodanese in Thiobacillus spp
Rhodanese (thiosulfate:cyanide sulfurtransferase; EC 2.8.1.1) catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. Conventional rhodanese assays colorimetrically measure the formation of one or the other of the products. These assays suffer from the fact that there is significant nonbiological formation of these products in additio
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6. Inhibition of the release factor-dependent termination reaction on ribosomes by DnaJ and the N-terminal peptide of rhodanese.
A peptide consisting of the 17 N-terminal amino acids of native bovine rhodanese in combination with the chaperone DnaJ specifically inhibits release factor- and stop codon-dependent hydrolysis of N-formylmethionine from N(formyl)-methionyl-tRNA bound with AUG to salt-washed ribosomes. Neither the peptide nor DnaJ by itself causes this inhibition. The N-term
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7. Observations on the Rhodanese Activity of Desulfotomaculum nigrificans
Rhodanese activity, partially purified from Desulfotomaculum nigrificans, was shown to reside in a protein fraction separate from thiosulfate reductase.
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8. Survey of the Photosynthetic Bacteria for Rhodanese (Thiosulfate: Cyanide Sulfur Transferase) Activity
Rhodanese activity was demonstrated in extracts from all three taxonomic families of photosynthetic bacteria, and this activity appeared to be uncorrelated with thiosulfate metabolism.
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9. Demonstration of Rhodanese Activity in Polyacrylamide Gels
Rhodanese activity from crude extracts of Thiobacillus sp. strain IV-85 was demonstrated in polyacrylamide gels after incubation in the reaction mixture by staining with dichloroindophenol in the presence of methylphenazonium methosulfate. The sensitivity of the staining system was found to be 8 × 10-7 moles of sulfite.
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10. Characterization of a 12-Kilodalton Rhodanese Encoded by glpE of Escherichia coli and Its Interaction with Thioredoxin
Rhodaneses catalyze the transfer of the sulfane sulfur from thiosulfate or thiosulfonates to thiophilic acceptors such as cyanide and dithiols. In this work, we define for the first time the gene, and hence the amino acid sequence, of a 12-kDa rhodanese from Escherichia coli. Well-characterized rhodaneses are comprised of two structurally similar ca. 15-kDa
American Society for Microbiology.
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11. Metabolism of Hydrogen Cyanide by Higher Plants 1
A survey has been made of the occurrence and distribution of three enzymes which metabolize cyanide in a variety of higher plants including both cyanogenic and non-cyanogenic species. The enzymes investigated were β-cyanoalanine synthase, rhodanese and formamide hydrolyase. β-Cyanoalanine synthase was found to be present in every higher plant tested wherea
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12. Observations on the Rhodanese Activity of Desulfotomaculum nigrificans