Reverse Phase Chromatography
Mostrando 1-12 de 441 artigos, teses e dissertações.
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1. In vitro anti-aging activities of ginkgo biloba leaf extract and its chemical constituents
Abstract To determine in vitro anti-aging activity and identify the active chemical constituents in Ginkgo leaf extracts. The antioxidant properties of Ginkgo biloba leaves extracts were evaluated using 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging and 2,2’-azino-bis (3-ethylbenzothiazoline)-6 sulfonic acid radical (ABTS) scavenging assays. The i
Food Sci. Technol. Publicado em: 2020-06
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2. Simultaneous determination of anti-diabetic drugs
A novel reverse phase, isocratic HPLC method is described to separate five anti-diabetic drugs i.e., glimepiride, metformin, sitagliptin, rosiglitazone and pioglitazone. Nucleosil C18 analytical column was used as stationary phase, while mobile phase consisted of acetonitrile:phosphate buffer: methanol (40/40/20, v/v) pH 2.0. Effluent was monitored at a flow
Braz. J. Pharm. Sci.. Publicado em: 20/12/2019
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3. Comparison of two collection methods to determine protodioscin concentrations in Brachiaria spp. and to monitor Brachiaria spp. Poisoning
RESUMO: Objetivou-se avaliar dois métodos de coleta de Brachiariaspp. (Pastejo Simulado vs Coleta Total) para determinação das concentrações de protodioscina e efetuar o monitoramento da intoxicação em rebanhos sensíveis e resistentes à intoxicação por Brachiaria spp. Foram realizadas coletas de Brachiariaspp. através de duas metodologias. A prim
Pesq. Vet. Bras.. Publicado em: 04/11/2019
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4. Rapid and Effective Sample Cleanup based on Polyvinylpyrrolidone-Coated Magnetite Nanoparticles Coupled with New Pre-Column Derivatization for Determination of Aflatoxins B1, B2, G1 and G2 in Animal Feeds by High-Performance Liquid Chromatography with Fluorescence Detection
A novel method for the determination of aflatoxins B1, B2, G1, and G2 (AFB1, AFB2, AFG1, AFG2) in animal feed samples based on magnetic solid-phase cleanup and novel pre-column derivatization has been developed. In this work, synthesized polyvinylpyrrolidone-coated Fe3O4 magnetic nanoparticles were applied as adsorbents for rapid cleanup for extracts of anim
J. Braz. Chem. Soc.. Publicado em: 16/09/2019
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5. Automatized Separation of Fractions from Petroleum Based on Spectrophotometric Signal Derivative Using Open-Source Hardware for the Determination of Ni and V Linked to Porphyrins
An automated method based on open-source hardware was created for the separation of porphyrins fractions from crude oil, an essential step for the quantification of V and Ni as metalloporphyrins. Preparative liquid chromatography was implemented with a reverse phase gradient from MeOH to toluene. The system allows three solvents and fractions changes by usin
J. Braz. Chem. Soc.. Publicado em: 23/05/2019
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6. Screening for organic acidurias and aminoacidopathies in high-risk Brazilian patients: Eleven-year experience of a reference center
Abstract Organic acidurias and aminoacidopathies are groups of frequent inborn errors of metabolism (IEMs), which are caused by mutations in specific genes that lead to loss of protein/enzyme or transport function with important deleterious effects to cell metabolism. Since a considerable number of such disorders are potentially treatable when diagnosed at a
Genet. Mol. Biol.. Publicado em: 11/04/2019
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7. Deep sequencing analysis of toad Rhinella schneideri skin glands and partial biochemical characterization of its cutaneous secretion
Abstract Background: Animal poisons and venoms are sources of biomolecules naturally selected. Rhinella schneideri toads are widespread in the whole Brazilian territory and they have poison glands and mucous gland. Recently, protein from toads’ secretion has gaining attention. Frog skin is widely known to present great number of host defense peptides and
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 17/12/2018
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8. Ajmalicine Bioproduction in Catharanthus Roseus (L) G. Don Inoculated with Arbuscular Mycorrhiza and Fertilized with Nitrogen
ABSTRACT Catharanthus roseus (L) G. Don (Madagascar periwinkle) belongs to the Apocynaceace family and is widely spread throughout tropical and subtropical regions of the world. The plant produces several important alkaloids, such as ajmalicine and serpentine, which are used in the treatment of circulatory diseases. The potential of inoculation with arbuscul
Rev. Bras. Ciênc. Solo. Publicado em: 23/07/2018
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9. PHENOLIC DERIVATIVES AND ANTIOXIDANT ACTIVITY OF POLAR EXTRACTS FROM Bauhinia pulchella
A tea made with the leaves or stem bark of Bauhinia pulchella Benth (Fabaceae) is used in the treatment of diabetes. Ethanol (EEF) and aqueous (EAF) extracts were obtained from the leaves and the antioxidant and citotoxic activities were tested, as well as quantify the content of flavonoids and phenolic compounds (TPC). EEF and EAF showed similar profiles by
Quím. Nova. Publicado em: 2018-04
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10. Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis
Abstract Background: Wasp venoms constitute a molecular reservoir of new pharmacological substances such as peptides and proteins, biological property holders, many of which are yet to be identified. Exploring these sources may lead to the discovery of molecules hitherto unknown. This study describes, for the first time in hymenopteran venoms, the identific
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 26/03/2018
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11. Development and validation of stability indicating liquid chromatographic (RP-HPLC) method for estimation of ubidecarenone in bulk drug and formulations using quality by design (QBD) approach
ABSTRACT A novel, accurate, precise and economical stability indicating Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) method, was developed and validated for the quantitative determination of ubidecarenone (UDC) in bulk drug, UDC marketed formulation and UDC loaded cubosomes (CBMs) nanocarriers through Response surface methodology (RSM) desi
Braz. J. Pharm. Sci.. Publicado em: 05/03/2018
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12. FOURIER TRANSFORM INFRARED SPECTROSCOPY, THERMOGRAVIMETRIC ANALYSIS, SCANNING ELECTRON MICROSCOPY AS SUPPORTING TOOLS IN QUALITY CONTROL OF ANTIPARASITICS
This study shows that Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), and scanning electron microscopy (SEM) can be used as supporting tools for the evaluation of the quality of antiparasitics. In addition, an analytical methodology was developed and validated to quantify simultaneously thiabendazole (TB), febantel (FB), tol
Quím. Nova. Publicado em: 2018-03