Resolvable
Mostrando 13-24 de 69 artigos, teses e dissertações.
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13. Structural details of membrane-bound acetylcholine receptor from Tropedo marmorata.
A projection, at 15- to 20-A resolution, is presented of the structure of the membrane-bound acetylcholine receptor protein from Torpedo marmorata. The projection has its axis perpendicular to the membrane plane; its main contribution originates from a hydrated portion of the protein, which extends from the membrane into the aqueous medium. The structure is
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14. Resolution of the DNA strands of the specialized transducing bacteriophage lambda-h80C 1-857 dargF.
The DNA strands of lambdoid phages with deletions or substitutions of the guanine plus cytosine-rich region in the left arm are not resolvable by complexing with poly UG followed by centrifugation in CsCl. This work describes a completely general procedure for the strand resolution of these phages by hybridization with fragments of separated strands of the p
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15. Multiphasic Uptake of Potassium by Corn Roots: No Linear Component
Concentration-dependence data (LV Kochian, WJ Lucas Plant Physiol 1982 70: 1723-1731; [1983] 73: 208-215; [1985] 429-436; LV Kochian, J Xin-zhi, WJ Lucas [1985], 79: 771-776) for potassium uptake by corn roots have been claimed to be resolvable into a saturable and a linear component representing two separate uptake mechanisms. A comprehensive analysis shows
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16. Carbon-13 nuclear magnetic resonance study of chondroitin 4-sulfate in the proteoglycan of bovine nasal cartilage.
The Fourier transform 13C nuclear magnetic resonance spectra of bovine nasal cartilage proteoglycan subunit and complex and whole bovine nasal cartilage were obtained and compared with that of chondroitin 4-sulfate. The spectrum of chondroitin 4-sulfate in solution revealed multiple resolvable resonances with linewidths that are consistent with considerable
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17. Chemical evidence that chromatin DNA exists as 160 base pair beads interspersed with 40 base pair bridges.
Digestion of rat liver nuclei by an endogenous endonuclease generates double-stranded DNA fragments which are initially about 205 base pairs long, as reported previously by Hewish and Burgoyne. As digestion proceeds, the average size of these fragments is reduced to about 160 base pairs. Electrophoresis under denaturing conditions shows that these DNA fragme
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18. Imaging of single molecule diffusion.
In recent years observations at the level of individual atoms and molecules became possible by microscopy and spectroscopy. Imaging of single fluorescence molecules has been achieved but has so far been restricted to molecules in the immobile state. Here we provide methodology for visualization of the motion of individual fluorescent molecules. It is applied
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19. A physical genome map of Pseudomonas aeruginosa PAO.
A complete macrorestriction map of the 5.9 Mb genome of Pseudomonas aeruginosa PAO (DSM 1707) was constructed by the combination of various one- and two-dimensional pulsed field gel electrophoresis techniques. A total of 51 restriction sites (36 SpeI sites, 15 DpnI sites) were placed on the physical map yielding an average resolution of 110 kb. Several genes
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20. Purification and biochemical properties of a bacteriocin from Actinobacillus actinomycetemcomitans.
Extracts of certain strains of Actinobacillus actinomycetemcomitans are inhibitory to strains of Streptococcus sanguis such as S. sanguis ATCC 10556. The isolation of a protein from an A. actinomycetemcomitans sonic extract which copurified with the inhibitory activity was accomplished by preparative isoelectric focusing, Sephadex G-100 gel filtration chroma
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21. Allelic discrimination by nick-translation PCR with fluorogenic probes.
Nick-translation PCR was performed with fluorogenic probes. Two probes were used: one complementary to a sequence containing the F508 codon of the normal human cystic fibrosis (CF) gene (wt DNA) and one complementary to a sequence containing the delta F508 three base pair deletion (mut DNA). Each probe contained a unique and spectrally resolvable fluorescent
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22. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes.
Dispersed repetitive DNA sequences have been described recently in eubacteria. To assess the distribution and evolutionary conservation of two distinct prokaryotic repetitive elements, consensus oligonucleotides were used in polymerase chain reaction [PCR] amplification and slot blot hybridization experiments with genomic DNA from diverse eubacterial species
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23. Combining solid-state and solution-state 31P NMR to study in vivo phosphorus metabolism.
Otherwise unavailable information concerning the distribution of phosphorylated compounds in biological systems is obtained by a combined solid-state/solution-state NMR approach, illustrated here for oocytes from Rana pipiens. General methodology is developed, and further extensions are proposed. The following conclusions pertain to the specific system under
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24. Symmetry activates extrastriate visual cortex in human and nonhuman primates
Humans often create and appreciate visual symmetry in their environment, and the underlying brain mechanisms have been a topic of increasing interest. Here, symmetric versus random dot stimuli produced robust functional MRI (fMRI) activity in higher-order regions of human visual cortex (especially areas V3A, V4, V7, and LO) but little activity elsewhere in b
National Academy of Sciences.