Replicon
Mostrando 1-12 de 659 artigos, teses e dissertações.
-
1. Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation
Abstract INTRODUCTION: Pseudo-infectious yellow fever viral particles (YFV-PIVs) have been used to study vaccines and viral packaging. Here, we report the development of a packaging cell line, which expresses the YFV prM/E proteins. METHODS: HEK293 cells were transfected with YFV prM/E and C (84 nt) genes to generate HEK293-YFV-PrM/E-opt. The cells were
Rev. Soc. Bras. Med. Trop.. Publicado em: 2018-02
-
2. An Insight Into the Intraspecific Variation of Biosynthetic Gene Clusters Between Strains of Burkholderia thailandensis spp.
The present study aimed to investigate the intraspecific variation of biosynthetic gene clusters (BGCs) in different strains of Burkholderia thailandensis in order to guide natural products (NPs) discovery process. Species from the genus Burkholderia are emerging as promising species due to their biosynthetic potential. Through genome-mining strategies, it w
J. Braz. Chem. Soc.. Publicado em: 2016-08
-
3. Characterization of the genetic environment of the bla KPC-2 gene among Klebsiella pneumoniae isolates from a Chinese Hospital
Abstract Infection caused by carbapenem-resistant Klebsiella pneumoniae has become a major healthcare threat and KPC-2 enzyme is a dominant factor mediating carbapenems resistance in K. pneumoniae. This study was designed to determine the genetic environment of bla KPC-2, which prevailed in clinical K. pneumoniae isolates recovered in Huashan Hospital, Shang
Braz J Infect Dis. Publicado em: 2016-08
-
4. Molecular characterization and epidemiology of cefoxitin resistance among Enterobacteriaceae lacking inducible chromosomal ampC genes from hospitalized and non-hospitalized patients in Algeria: description of new sequence type in Klebsiella pneumoniae isolates
In this study, 922 consecutive non-duplicate clinical isolates of Enterobacteriaceae obtained from hospitalized and non-hospitalized patients at Bejaia, Algeria were analyzed for AmpC-type β-lactamases production. The ampC genes and their genetic environment were characterized using polymerase chain reaction (PCR) and sequencing. Plasmid incompatibility gro
Braz J Infect Dis. Publicado em: 2015-04
-
5. Construction and characterisation of a complete reverse genetics system of dengue virus type 3
Dengue virulence and fitness are important factors that determine disease outcome. However, dengue virus (DENV) molecular biology and pathogenesis are not completely elucidated. New insights on those mechanisms have been facilitated by the development of reverse genetic systems in the past decades. Unfortunately, instability of flavivirus genomes cloned in E
Mem. Inst. Oswaldo Cruz. Publicado em: 11/10/2013
-
6. Construction of yellow fever virus subgenomic replicons by yeast-based homologous recombination cloning technique
O replicon de RNA derivado do genoma de Flavivirus é uma ferramenta valiosa para o estudo de replicação viral independente da montagem e da maturação do virion, além de possuir um grande potencial para expressão de genes heterólogos. Neste estudo nós descrevemos a construção de replicons subgenômicos do vírus da febre amarela utilizando a técni
An. Acad. Bras. Ciênc.. Publicado em: 01/03/2013
-
7. ACONSELHAMENTO GENÉTICO: ANÁLISE E CONTRIBUIÇÕES A PARTIR DO MODELO DO ACONSELHAMENTO PSICOLÓGICO
O AG é definido como um processo de investigação clínica, voltado para o diagnóstico de uma condição genética e que visa à orientação sobre o prognóstico e riscos de ocorrências/recorrências das doenças genéticas para as famílias e/ou para os consulentes. O presente estudo teve por objetivo a análise do modelo de Aconselhamento Genético (A
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 10/01/2011
-
8. ANÁLISE DO POLIMORFISMO GENÉTICO DO CÓDON 72 DO GENE P53 EM PACIENTES COM CARCINOMA ESCAMOSO DE BASE DA LÍNGUA
INTRODUÇÃO: O polimorfismo no códon 72, prolina (p53P) ou arginina (p53R) está envolvido na habilidade da p53 em interagir com as proteínas celulares. Vários autores têm demonstrado que a presença do genótipo p53RR confere maior risco de desenvolvimento de tumores. OBJETIVO: Avaliar a freqüência alélica do polimorfismo genético no códon 72 do g
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 05/05/2009
-
9. Participation of the lytic replicon in bacteriophage P1 plasmid maintenance.
P1 bacteriophage carries at least two replicons: a plasmid replicon and a viral lytic replicon. Since the isolated plasmid replicon can maintain itself stably at the low copy number characteristic of intact P1 prophage, it has been assumed that this replicon is responsible for driving prophage replication. We provide evidence that when replication from the p
-
10. Replication Studies Using Genotype 1a Subgenomic Hepatitis C Virus Replicons
Recently, cell-based replicon systems for hepatitis C virus (HCV), in which the nonstructural proteins stably replicate subgenomic viral RNA in Huh7 cells, were developed. To date, one limitation of using these replicon systems to advance drug discovery is the inability of other genotypic derivatives, beyond those of two distinct strains of genotype 1b (HCV-
American Society for Microbiology.
-
11. An Alphavirus Replicon Particle Chimera Derived from Venezuelan Equine Encephalitis and Sindbis Viruses Is a Potent Gene-Based Vaccine Delivery Vector
Alphavirus replicon particle-based vaccine vectors derived from Sindbis virus (SIN), Semliki Forest virus, and Venezuelan equine encephalitis virus (VEE) have been shown to induce robust antigen-specific cellular, humoral, and mucosal immune responses in many animal models of infectious disease and cancer. However, since little is known about the relative po
American Society for Microbiology.
-
12. Regulation of Hepatitis C Virus Replication by Interferon Regulatory Factor 1
Cellular antiviral responses are mediated partly by the expression of interferon-stimulated genes, triggered by viral genomes, their transcripts and replicative intermediates. Persistent replication of a hepatitis C virus (HCV) replicon suggests that the replicon does not elicit cellular innate antiviral responses. In the present study, we investigated regul
American Society for Microbiology.