Replication Sites
Mostrando 13-24 de 2078 artigos, teses e dissertações.
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13. Lattice model of replicators: aplication on prebiotic models and herpes ulcer / Dinâmica de replicação na rede: aplicações em modelos de evolução pré-biótica e de formação de úlceras
Two fundamental questions in the study of prebiotic evolution (origin of life) are concerned to the requisites for the persistence of small colonies of self-replicating molecules (replicators) and to the possibility that complex organisms evolve from simpler organisms as a result of mutations. These issues have been studied mainly in the chemical kinetics fo
Publicado em: 2001
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14. Adenovirus replication and transcription sites are spatially separated in the nucleus of infected cells.
We have visualized the intranuclear topography of adenovirus replication and transcription in infected HeLa cells. The results show that viral DNA replication occurs in multiple foci that are highly organized in the nucleoplasm. Pulse-chase experiments indicate that newly synthesized viral double-stranded DNA molecules are displaced from the replication foci
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15. DNA replication of histone gene repeats in Drosophila melanogaster tissue culture cells: multiple initiation sites and replication pause sites.
We showed previously that DNA replication initiates at multiple sites in the 5-kb histone gene repeating unit in early embryos of Drosophila melanogaster. The present report shows evidence that replication in the same chromosomal region initiates at multiple sites in tissue culture cells as well. First, we analyzed replication intermediates by the two-dimens
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16. Nuclear sites of herpes simplex virus type 1 DNA replication and transcription colocalize at early times postinfection and are largely distinct from RNA processing factors.
We have visualized the intracellular localization of herpes simplex virus (HSV) type 1 replication and transcription sites in infected HeLa cells by using direct labelling methods. The number of viral transcription foci increases in a limited way; however, the number of replication sites increases in a near-exponential manner throughout infection, and both r
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17. DNA Replication Efficiency Depends on Transcription Factor-Binding Sites
Naturally arising variants of simian virus 40 (SV40), generated by serial passage of the virus at high multiplicities of infection, provide important insight into the role of transcription factor-binding sites in enhancing DNA replication. Although the variants that arise from numerous recombination events are the result of selective pressure to replicate mo
American Society for Microbiology.
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18. Start sites for bidirectional in vitro DNA replication inside the replication origin, oriC, of Escherichia coli.
In vitro replication of mini-chromosomes in the absence of DNA ligase activity resulted in replication products with single-strand breaks at specific sites. The occurrence of these nicks was coupled to an active replication process, therefore we expect them to represent start sites for DNA replication. Two positions within oriC for each of the two leading st
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19. Cell cycle regulator phosphorylation stimulates two distinct modes of binding at a chromosome replication origin
In Caulobacter crescentus, the global response regulator CtrA controls chromosome replication and determines the fate of two different cell progenies. Previous studies proposed that CtrA represses replication by binding to five sites, designated [a–e], in the replication origin. We show that phosphorylated CtrA binds sites [a–e] with 35- to 100–fold lo
Oxford University Press.
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20. Correlation between premeiotic DNA replication and chromatin transition at yeast recombination initiation sites
The DNA double-strand breaks (DSBs) that initiate meiotic recombination in Saccharomyces cerevisiae are preceded first by DNA replication and then by a chromatin transition at DSB sites. This chromatin transition, detected as a quantitative increase in micrococcal nuclease (MNase) sensitivity, occurs specifically at DSB sites and not at other MNase-sensitive
Oxford University Press.
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21. The replication activation potential of selected RNA polymerase II promoter elements at the simian virus 40 origin.
Binding sites for cellular transcription factors were placed near the simian virus 40 origin of replication, and their effect on replication and TATA-dependent transcription was measured in COS cells. The hierarchy of transcriptional stimulation changed when the plasmids replicated. Only one of seven inserted sequences, a moderately weak transcription elemen
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22. Topoisomerase I sites cluster asymmetrically at the ends of the simian virus 40 core origin of replication.
In vivo, topoisomerase I cleavage sites are located predominantly on the strands of simian virus 40 DNA that are the templates for discontinuous synthesis (S.E. Porter and J.J. Champoux, Mol. Cell. Biol. 9:541-550, 1989). This arrangement of sites suggests that topoisomerase I may associate with replication complexes in unique functional orientations at repl
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23. cis-Acting components of human papillomavirus (HPV) DNA replication: linker substitution analysis of the HPV type 11 origin.
Papillomavirus DNA replication requires the viral trans-acting factors E1 and E2 in addition to the host cell's general replication machinery. The origins of DNA replication in bovine and human papillomavirus genomes have been localized to a specific part of the upstream regulatory region (URR) which includes recognition sites for E1 and E2 proteins. To fine
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24. CCAAT/enhancer binding protein (C/EBP) sites are required for HIV-1 replication in primary macrophages but not CD4+ T cells
The importance of CCAAT/enhancer binding proteins (C/EBPs) and binding sites for HIV-1 replication in primary macrophages, T cell lines and primary CD4+ T cells was examined. When lines overexpressing the C/EBP dominant-negative protein LIP were infected with HIV-1, replication occurred in Jurkat T cells but not in U937 promonocytes, demonstrating a requirem
The National Academy of Sciences of the USA.