Regulatory Tendency
Mostrando 13-24 de 32 artigos, teses e dissertações.
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13. NMR structure of a biologically active peptide containing the RNA-binding domain of human immunodeficiency virus type 1 Tat.
The Tat protein of human immunodeficiency virus type 1 enhances transcription by binding to a specific RNA element on nascent viral transcripts. Binding is mediated by a 10-amino acid basic domain that is rich in arginines and lysines. Here we report the three-dimensional peptide backbone structure of a biologically active 25-mer peptide that contains the hu
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14. Self-association of the Drosophila zeste protein is responsible for transvection effects.
The zeste gene product is required for transvection effects that imply the ability of regulatory elements on one chromosome to affect the expression of the homologous gene in a somatically paired chromosome. The z1 mutation causes a pairing dependent inhibition of the expression of the white gene. Both of these phenomena can be explained by the tendency of z
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15. Ligand-promoted weakening of intersubunit bonding domains in aspartate transcarbamoylase
The cooperativity and feedback inhibition exhibited by the regulatory enzyme, aspartate transcarbamoylase (carbamoylphosphate: L-aspartate carbamoyltransferase; EC 2.1.3.2), from Escherichia coli are generally attributed to ligand-promoted conformational changes involving alterations in the subunit interactions. However, no quantitative estimates have been m
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16. The role of certain Post classes in Boolean network models of genetic networks
A topic of great interest and debate concerns the source of order and remarkable robustness observed in genetic regulatory networks. The study of the generic properties of Boolean networks has proven to be useful for gaining insight into such phenomena. The main focus, as regards ordered behavior in networks, has been on canalizing functions, internal homoge
National Academy of Sciences.
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17. Adaptive significance of differences in the tissue-specific expression of a phosphoglucomutase gene in rainbow trout.
We have investigated the phenotypic effects of a mutant allele that results in the expression of a phosphoglucomutase locus (Pgm1) in the liver of rainbow trout. Embryos with liver Pgm1 expression hatch earlier than embryos without liver Pgm1 expression. These differences apparently result from increased flux through glycolysis in embryos with liver PGM1 act
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18. Zinc transfer from transcription factor IIIA fingers to thionein clusters.
The rapid induction of thionein (apometallothionein) by many endogenous stimuli such as steroid hormones, cytokines, and second messengers suggests that this cysteine-rich, metal binding protein participates in an as yet undefined role in cellular regulatory processes. This study demonstrates with DNA and RNA binding assays and in vitro transcription measure
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19. Use of the Trypanosoma cruzi Recombinant Complement Regulatory Protein To Evaluate Therapeutic Efficacy following Treatment of Chronic Chagasic Patients
One of the greatest concerns in Chagas' disease is the absence of reliable methods for the evaluation of chemotherapy efficacy in treated patients. The tests available to evaluate cure after the specific treatment are the complement-mediated lysis (CoML) and flow cytometry tests, but they are not feasible for routine clinical use. In this study, we evaluated
American Society for Microbiology.
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20. RNA-DNA hybridization promoted by E. coli RecA protein.
RecA protein of E. coli plays a central regulatory role that is induced by damage to DNA and results in the inactivation of LexA repressor. In vitro, RecA protein binds preferentially to single-stranded DNA to form a nucleoprotein filament that can recognize homology in naked duplex DNA and promote extensive strand exchange. Although RecA protein shows littl
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21. Effects of yeast DNA topoisomerase III on telomere structure.
The yeast TOP3 gene, encoding DNA topoisomerase III, and EST1 gene, encoding a putative telomerase, are shown to be abutted head-to-head on chromosome XII, with the two initiation codons separated by 258 bp. This arrangement suggests that the two genes might share common upstream regulatory sequences and that their products might be functionally related. A c
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22. Bacterial-type Phosphoenolpyruvate Carboxylase (PEPC) Functions as a Catalytic and Regulatory Subunit of the Novel Class-2 PEPC Complex of Vascular Plants*
Phosphoenolpyruvate carboxylase (PEPC) is a tightly regulated anaplerotic enzyme situated at a major branch point of the plant C metabolism. Two distinct oligomeric classes of PEPC occur in the triglyceride-rich endosperm of developing castor oil seeds (COS). Class-1 PEPC is a typical homotetramer composed of identical 107-kDa plant-type PEPC (PTPC) subunits
American Society for Biochemistry and Molecular Biology.
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23. Structural analysis of adenylate cyclases from Trypanosoma brucei in their monomeric state
Cyclic AMP is a major trigger of the differentiation process of Trypanosoma brucei, a bloodstream parasite causing sleeping sickness. Its generation in trypanosomes is accomplished by a unique battery of membrane-bound adenylate cyclases (ACs). We have determined the high-resolution X-ray structures of the catalytic domains of two trypanosomal ACs (tACs), GR
Oxford University Press.
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24. Constant and variable parts in the Balbiani ring 2 repeat unit and the translation termination region
The large Balbiani rings of the chironomids produce giant internally repeated transcripts that are translated into silk-like proteins used for protective tubes. We have cloned fragments of the Balbiani ring 2 (BR2) gene of Chironomus pallidivittatus, normally the most prominent BR, for sequencing and restriction analysis. The results indicate a basic, tandem