Recombinant Protein
Mostrando 13-24 de 8143 artigos, teses e dissertações.
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13. Improved antifungal activity of barley derived chitinase I gene that overexpress a 32 kDa recombinant chitinase in Escherichia coli host
Abstract Agricultural crops suffer many diseases, including fungal and bacterial infections, causing significant yield losses. The identification and characterisation of pathogenesis-related protein genes, such as chitinases, can lead to reduction in pathogen growth, thereby increasing tolerance against fungal pathogens. In the present study, the chitinase I
Braz. J. Microbiol.. Publicado em: 2018-06
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14. Rapid detection of food-borne Salmonella contamination using IMBs-qPCR method based on pagC gene
Abstract Detection of Salmonella is very important to minimize the food safety risk. In this study, the recombinant PagC protein and PagC antibody were prepared and coupled with immunomagnetic beads (IMBs) to capture Salmonella cells from pork and milk samples. And then the SYBR Green qualitative PCR was developed to detect the pathogenic Salmonella. The res
Braz. J. Microbiol.. Publicado em: 2018-06
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15. Epoetin alfa resistance in hemodialysis patients with chronic kidney disease: a longitudinal study
Anemia is an inevitable complication of hemodialysis, and the primary cause is erythropoietin deficiency. After diagnosis, treatment begins with an erythropoiesis-stimulating agent (ESA). However, some patients remain anemic even after receiving this medication. This study aimed to investigate the factors associated with resistance to recombinant human eryth
Braz J Med Biol Res. Publicado em: 07/05/2018
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16. Distribution of Ifitm3 in Yellow-Feathered Broilers and Inhibition of Avian Reovirus Multiplication by Ifitm3
ABSTRACT This study was carried to express the interferon-induced transmembrane protein 3 (IFITM3) in vitro and examine its function in inhibition of avian reovirus (ARV) replication. The recombinant prokaryotic vector expressing yellow-feathered broiler IFITM3 was successfully constructed, and the recombinant protein was expressed in competent Escherichia c
Braz. J. Poult. Sci.. Publicado em: 2018-04
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17. Down-regulated miR-448 relieves spinal cord ischemia/reperfusion injury by up-regulating SIRT1
MicroRNAs play a crucial role in the progression of spinal cord ischemia/reperfusion injury (SCII). The role of miR-448 and SIRT1 in SCII was investigated in this study, to provide further insights into prevention and improvement of this disorder. In this study, expressions of miR-448 and SIRT1 protein were determined by qRT-PCR and western blot, respectivel
Braz J Med Biol Res. Publicado em: 15/03/2018
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18. Potential application of rLc36 protein for diagnosis of canine visceral leishmaniasis
Visceral leishmaniasis (VL) is fatal if left untreated. Infected dogs are important reservoirs of the disease, and thus specific identification of infected animals is very important. Several diagnostic tests have been developed for canine VL (CVL); however, these tests show varied specificity and sensitivity. The present study describes the recombinant prote
Mem. Inst. Oswaldo Cruz. Publicado em: 2018-03
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19. Jaburetox: update on a urease-derived peptide
Abstract Urease from Canavalia ensiformis seeds was the first enzyme ever to be crystallized, in 1926. These proteins, found in plants, bacteria and fungi, present different biological properties including catalytic hydrolysis of urea, and also enzyme-independent activities, such as induction of exocytosis, pro-inflammatory effects, neurotoxicity, antifungal
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 08/02/2018
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20. Collagen sponge and rhBMP-2 improve socket healing in rats treated with zoledronic acid
Abstract The aim of the present study was to evaluate the possible use of a commercial absorbed collagen sponge and bone morphogenetic protein (BMP) for the prevention of bisphosphonate-related osteonecrosis of the jaw (BRONJ) in rats. Twenty rats received intraperitoneal injections of 0.1-mg/kg of zoledronic acid three times a week for eight weeks before th
Braz. oral res.. Publicado em: 07/12/2017
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21. A novel expression vector for the secretion of abaecin in Bacillus subtilis
ABSTRACT This study aimed to describe a Bacillus subtilis expression system based on genetically modified B. subtilis. Abaecin, an antimicrobial peptide obtained from Apis mellifera, can enhance the effect of pore-forming peptides from other species on the inhibition of bacterial growth. For the exogenous expression, the abaecin gene was fused with a tobacco
Braz. J. Microbiol.. Publicado em: 2017-12
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22. Isolation and characterization of a novel endo-β-1,4-glucanase from a metagenomic library of the black-goat rumen
ABSTRACT The various types of lignocellulosic biomass found in plants comprise the most abundant renewable bioresources on Earth. In this study, the ruminal microbial ecosystem of black goats was explored because of their strong ability to digest lignocellulosic forage. A metagenomic fosmid library containing 115,200 clones was prepared from the black-goat r
Braz. J. Microbiol.. Publicado em: 2017-12
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23. Parenteral adjuvant potential of recombinant B subunit of Escherichia coli heat-labile enterotoxin
BACKGROUND The B subunit of Escherichia coli heat-labile enterotoxin (LTB) is a potent mucosal immune adjuvant. However, there is little information about LTB's potential as a parenteral adjuvant. OBJECTIVES We aimed at evaluating and better understanding rLTB's potential as a parenteral adjuvant using the fused R1 repeat of Mycoplasma hyopneumoniae P97 ad
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-12
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24. Imunogenicidade da proteína M recombinante de Streptococcus equi subsp. equi coadministrada com um adjuvante molecular
ABSTRACT The strangles is an infectious disease that affects horses from all ages and causes important economic losses in the equine-related business. The aim of this work was to evaluate the immunogenicity of the recombinant M protein from Streptococcus equi (rSeM) co-administered with the recombinant heat-labile enterotoxin B subunit from Escherichia coli
Arq. Bras. Med. Vet. Zootec.. Publicado em: 2017-10